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==Principle==
 
==Principle==
*The general priniciple behind the RIA is the competitive binding of a radiolabelled antigen and unlabelled antigen to a high-affinity antibody.
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*The general priniciple behind the RIA is the competitive binding of a radiolabeled antigen and unlabeled antigen to a high-affinity antibody.
*Labelled antigen is mixed with antibody until binding sites are saturated
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*Labeled antigen is mixed with antibody until binding sites are saturated
*Samples of unlabelled antigen (unknown concentration) are added in progressively increasing amounts  
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*Samples of unlabeled antigen (unknown concentration) are added in progressively increasing amounts  
 
**The two different antigens compete for the binding sites on the antibody
 
**The two different antigens compete for the binding sites on the antibody
**As the concentration of unlabelled antibody increases, more labelled antigen will be displaced
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**As the concentration of unlabeled antibody increases, more labeled antigen will be displaced
*The decrease in bound-radiolabelled antigen is measured- an indication of the amount of antigen present in sample
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*The decrease in bound-radiolabeled antigen is measured - an indication of the amount of antigen present in sample
    
==Technique==
 
==Technique==
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