In Vitro Maturation - Anatomy & Physiology

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  • Of interest for breeding guide dogs, which are monoetrous animals that do not produce many mature oocytes. This is desirable to avoid bitches having to take leave due to pregnancy.
  • Experimental with low success rates.
  • Routine in mice.
  • Recovery of secondary or primary (ultimately primordial) follicles.
  • Oocytes are arrested in the germinal vesicle stage (prophase of the first meiotic division).
  • Follicles matured in vitro for subsequent IVF.
  • Donor should be pre-pubertal, thus a full pool of primordial follicles is available.