Male Reproductive Tract - Horse Anatomy

Testes and Epididymis

Scrotum

Outer-most layer is the scrotal skin, which contains many sweat glands, thermosensitive receptors and sympathetic nerves. The tunica dartos is a smooth muscle layer found below the skin of the scrotum. In horses, this muscle forms a septum which divides the scrotal sac into right and left compartments. Smooth muscle tone is controlled by androgens released in response to temperature changes, detected by sensory nerves within the scrotal skin. The tunica dartos is responsible for holding the testes close to the body during cold temperatures, as it can maintain sustained contraction, and letting the testes lower in hot weather, increasing the surface area for heat loss from the skin. The next layer is the scrotal fascia.

The testes are contained within the tunica albiguinea, which is a fibrous capsule composed of collagen. It also contains the testicularis arteries and veins, which are visible on the surface of the testicle. This capsule is covered by the tunica vaginalis, which is an outpocketing of the abdominal peritoneum. The tunica vaginalis can be further divided into the visceral and parietal tunics. The visceral tunic is adherent to the testicle, ducts and vessels. The parietal tunic is continuous with the parietal peritoneum. The parietal tunic adheres to the tunica dartos muscle, scrotal fascia and scrotal skin. The tunica albuginea sends projections from the capsules into the testicular parenchyma that join up with the mediastinum of the testis forming lobules. Contraction and relaxation of the smooth muscle fibres within the tunica albuginea in response to acetylcholine and norepinephrine results in a pumping action facilitating the flow of spermatozoa through the tubules of the testes.

The cremaster muscle is the caudolateral extension of the internal abdominal oblique muscle. It is continous with the parietal layer of the tunica vaginalis and inserts at the caudal pole of the testes.

The scrotum has important functions:

• Temperature sensor
• Protection
• Cooling of the testes - evaporative heat loss due to lots of sweat produced by glands in the skin.

Testes

In the horse, the left testicle is usually larger and more caudal than the right testicle. It is also usually suspended more ventrally.

The parenchyma of the testes is composed of the semniferous tubules, rete testis and efferent ducts. The semniferous tubules are the site of spermatogenesis. The walls of the semniferous tubules contain spermatogenic cells and their sustentacular cells, which are known as sertoli cells. The sertoli cells give support and also produce hormones. They regulate spermatogenesis and the release of spermatozoa into the lumen of the semniferous tubules. Each semniferous tubule opens into a network of tubules called the rete testis. Each rete testis is drained by multiple efferent ducts which perforate the capsule of the testes to enter the head of the epididymis. The interstitial tissue between semniferous tubules contains Leydig cells. These cells produce androgens, such as testosterone.

The Blood-Testes Barrier is formed by tight junctions between neighbouring Sertoli cells. This barrier prevents leukocytes from entering the adluminal compartment of the seminipherous tubules and autoreacting to the haploid spermatids within it.

Epididymis

The Epididymis provides the environment for final maturation of spermatozoa. It is here that they aquire motility and fertility. The Epididymal duct is very long, between 72-81 meters in the horse. It is divided functionally into 3 regions; the Caput (head), Corpus (body) and Cauda (tail). The caput of the epididymis is connected to the efferent ducts of the testes. The Cauda connects to the Ductus Deferens of the spermatic cord.

Caput (Head)

Attached to the testicular capsule, receives the efferent ducts. After efferent ducts enter the head of the epididymis, they join to become the duct of the epididymis. Fluid secreted in the rete tubules of the testes is reabsorbed here and epididymal fluid is secreted. This fluid secretion is important in order to facilitate movement of the sperm through the epididymis.

Corpus (Body)

Formed by the convuluted ducts. Nuclear chromatin condenses via the addition of di-sulphide bridges. This condensation stabilises the nuclear material and can be observed histologically as darker staining material. Stabilising surface proteins are added to the sperm membranes. Sperm aquire fertility by the addition of membrane receptors to female tract ligands. This enables them to bind to the zona pellucida and oocyte membranes. Sperm cells are dehydrated and sperm acquire forward motility.

Cauda (tail)

Continuation of the duct of the epididymis, which continues as the ductus deferens. It is attached to the caudal pole of the testis by the proper ligament of the testis and to the vaginal process by the ligament of the tail of the epidymis. Sperm metabolic activity is depressed in order to prolong life as they are stored here until ejaculation.

Histology

The Epididymal duct is surrounded by smooth muscle fibers. These fibers contract rhythmically facilitating sperm movement through the duct.

Caput

• Columna epithelium with basal nuclei.
• Small steriocillia present, these have an absorbtive function

Corpus

• Low columna epithelium with very prominant stereocillia.
• Small groups of sperm can be observed within the lumen of the duct.

Cauda

• Cuboidal/ Low columna epithelium. However, unlike the caput and corpus regions of the epididymis the cauda epithelium has no stereocillia.
• Large numbers of sperm observable here as this is the site of sperm storage within the testes.

Ductus Deferens

The ductus deferens is a direct continuation of the duct of the epididymis from the cauda (tail) of the epididymis. It runs cranially within the spermatic cord to enter the abdominal cavity via the inguinal canal. It loops within a fold of peritoneum and passes under the ureter to reach the dorsal surface of the bladder. It enters the prostate gland and opens at the seminal hillock in the proximal urethra. The ampulla is a thickening that arises at the termination of the ductus deferens adjacent to the ampullary gland. In the horse, the excretory duct of the vesicular gland joins the ductus deferens close to its termination. The shared duct is known as the ejaculatory duct.

Spermatic Cord

The spermatic cord comprises the ductus deferens, testicular vessels and nerves. It runs from the abdomen caudally, through the inguinal canal. The inguinal canal is formed by the deep and superficial inguinal rings.

• Deep inguinal ring: Opening between the internal abdominal oblique, rectus abdominis, prepubic tendon and inguinal ligament.
• Superficial inguinal ring: Opening in the external abdominal oblique.