Brucella species

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• B. abortus occasionally in arthritis of cattle

• Isolated from closed cases of Poll Evil and Fistulous Withers

Overview

• Important zoonoses worldwide
• Cause chronic granulomatous diseases
• 6 species
• Target reproductive organs of certain species
• Infected animals act as reservoir of infection
• Organisms can remain viable in moist environment for months
• Cause undulant fever in humans

Characteristics

• Small, non-moltile, Gram negative coccobacilli
• Facultatice intracellular pathogens
• Modified Ziehl-Neelsen positive - clusters of red coccobacilli on smears
• Aerobic and capnophilic
• Catalase positive; oxidase and urease positive except for Brucella ovis
• Some species require enriched media for growth
• Non-haemolytic
• Smooth colonies of B. abortus, B. melitensis and B. suis are small, glistening, blue and translucent after incubation for 3-5 days, and become opaque with age
• Rough colonies of B. ovis and B. canis are dull, yellow, opaque and friable
• Slide agglutination with speicific antisera detect important antigens
• B. abortus lysed by specific bacterophages
• Oxidative metaboloic rates can differentiate species

Pathogenesis and pathogenicity

• Brucellae that lack outer membrane LPS (rough colonies) are less virulent than those which possess it
• Penetrate nasal, oral or pharyngeal mucosa
• Phagocytosed and carried to regional lymph nodes
• Smooth organisms survive and multiply in cells of the reticulo-endothelial system
• Inhibit lysosome-phagosome fusion
• Superoxide dismutase and catalase production may resist oxidative killing
• Lymph nodes enlarge (lymphatic and lymphoreticular hyperplasia) and inflammation is induced
• Surviving organisms spread to other organs (liver, spleen, placenta) and cause granulomatous reactions
• Eythritol is a growth stimulant and attracts the bacteria to the placenta of cattle, sheep, goats and pigs; also found in mammary gland and epididymis, targets for brucellae
• Infection of foetus and abortion
• May localise in joints or intervertebral discs in chronic infections

Clinical disease

• Bovine brucellosis:
  • Caused by Brucella abortus
  • Eradicated in many countries including UK
  • Infection usually by ingestions but also venereal, skin abrasions, inhalation, transplacental
  • Abortion storms in susceptible herds
  • Abortion after fifth month of gestation due to placentitis
  • Brucellae excreted in foetal fluids for 2-4 weeks following abortion and at subsequent parturitions without abortion
  • Infection of mammary glands and lymph nodes persists for years
  • Excreted intermittently in milk for years
  • Seminal vesicles, ampullae, testicles and epididymus infected in bulls; necrotising orchitis
  • Decreased fertility and milk production

Diagnosis

• Serological testing of milk (Milk Ring Test) and beef cattle (Rose Bengal Plate Test)
• Serological tests detect anti-lipopolysaccharide antibodies
• LPS antigen present in virulent as well as some vaccine strains therefore vaccination may confuse serological testing
• False positives due to cross-reaction with LPS in other bacteria
• Modified Ziehl-Neelson stains reveal organisms in samples from cotyledons, uterine discharge and foetal abomasal contents
• PCR for detection in tissue
• Enriched media for isolation

Control

• Vaccination:
  • Strain S19, a live vaccine, stimulating a cell-mediated immune response; vaccination of young animals; interferes with serological testing
  • Newer RB51 vaccine has no LPS O-antigen therefore not detcted by serological tests
  • Live attenuated Brucella melitensis vaccine to protect sheep and goats against B. melitensis
• Test and slaughter program has eradicated Brucellosis in the UK