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[[Image:Secondary Follicle.jpg|thumb|right|150px|Histological Section of the Ovary showing Characteristics of the Secondary Follicle- Courtesy of J.Bredl, Copyright RVC 2008]]
 
[[Image:Secondary Follicle.jpg|thumb|right|150px|Histological Section of the Ovary showing Characteristics of the Secondary Follicle- Courtesy of J.Bredl, Copyright RVC 2008]]
*The oocyte had developed a thick translucent glycoprotein layer called the Zona Pellucida.
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*The oocyte has developed a thick translucent glycoprotein layer called the Zona Pellucida.
 
*Oocyte surrounded by two or more layers of follicular cuboidal cells. These cells differentiate into the inner Granulosa cells and outer Theca cells.
 
*Oocyte surrounded by two or more layers of follicular cuboidal cells. These cells differentiate into the inner Granulosa cells and outer Theca cells.
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* Connective tissue cells surrounding the granulosa cells differentiate to form the layers of theca cells which produce androgens.
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** Androgens act as substrates for synthesis of oestrogens by granulosa cells.
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* Granulosa cells secrete
     
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