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[[Image:Tertiary Follicle.jpg|thumb|right|150px|Histological Section of the Ovary showing Characteristics of the Tertiary/Antral/Graafian Follicle- Courtesy of J.Bredl, Copyright RVC 2008]]
 
[[Image:Tertiary Follicle.jpg|thumb|right|150px|Histological Section of the Ovary showing Characteristics of the Tertiary/Antral/Graafian Follicle- Courtesy of J.Bredl, Copyright RVC 2008]]
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*The antrum (filled with follicular fluid) is characteristic of antral/tertiary follicles.
 
*The innermost cells of the Granulosa layer become firmly attached to the Zona Pellucida and are known as the Corona Radiata. The outermost Granulosa cells become attached to the basement membrane surrounding them and become known as the Theca Folliculi.
 
*The innermost cells of the Granulosa layer become firmly attached to the Zona Pellucida and are known as the Corona Radiata. The outermost Granulosa cells become attached to the basement membrane surrounding them and become known as the Theca Folliculi.
*Granulosa cells secrete fluid which accumulates within the follicle forming an Antrum.
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*Theca cells become organised into Theca Interna and Theca Externa layers.  
*Theca cells become organised into Theca Interna and Theca Externa layers. The Theca Interna layer cells differenciate into steroidogenic cells produce the Androgen Androsteinedione in response to the Gonadotrophin Luteinising hormone (LH).
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*The Theca Interna layer cells differenciate into steroidogenic cells produce the Androgen Androsteinedione in response to the Gonadotrophin Luteinising hormone (LH).
 
*Theca Cell layer becomes vascularised.
 
*Theca Cell layer becomes vascularised.
*Granulosa cells become responsive to Follicle Stimulating Hormone (FSH) and start producing [[Endocrine_System_-_Ovaries_-_Anatomy_%26_Physiology|17β-Oestrodiol]] and small amounts of Inhibin and Progesterone.
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