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Immunological Testing Flashcards (view source)

Revision as of 17:11, 17 March 2012

2,100 bytes removed ,  17:11, 17 March 2012
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==<font color="purple">ELISA testing</font>==
 
==<font color="purple">ELISA testing</font>==
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{| border="3" cellpadding="8"
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<FlashCard questions="3">
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!width="400"|'''Question'''
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|q1=What does the acronym ELISA stand for?
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!width="400"|'''Answer'''
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|a1=Enzyme Linked ImmunoSorbent Assay
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!width="150"|'''Article'''
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|l1=ELISA testing
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|-
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|q2=What type of ELISA requires all the reagents to be added at once?
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|<big>'''What does the acronym ELISA stand for?'''
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|a2=Homogenous
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||<font color="white"> <big>
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|l2=ELISA testing
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*'''''Enzyme Linked ImmunoSorbent Assay'''''
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|q3=What are the five basic steps of an ELISA test?
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||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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|a3=
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|-
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*Adsorption of antigen/antibody onto solid phase
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|<big>'''What type of ELISA requires all the reagents to be added at once?'''
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*Addition of chosen sample and reagents
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||<font color="white"> <big>
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*Incubation and washing
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*'''''Homogenous'''''
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*Addition of enzyme-labelled antigen/antibody
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||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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*Addition of specific substrate
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|-
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|l3=ELISA testing
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|<big>'''What are the five basic steps of an ELISA test?'''
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</FlashCard>
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||<font color="white"> <big>
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*'''''Adsorption of antigen/antibody onto solid phase'''''
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*'''''Addition of chosen sample and reagents'''''
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*'''''Incubation and washing'''''
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*'''''Addition of enzyme-labelled antigen/antibody'''''
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*'''''Addition of specific substrate'''''
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||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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|}
   
==<font color="purple">Immunofluorescence</font>==
 
==<font color="purple">Immunofluorescence</font>==
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{| border="3" cellpadding="8"
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<FlashCard questions="4">
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!width="400"|'''Question'''
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|q1=The adsorption of light by a molecule is known as what?
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!width="400"|'''Answer'''
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|a1=Excitation
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!width="150"|'''Article'''
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|l1=Immunofluorescence
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|-
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|q2=What are the fluorescent dyes commonly known as?
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|<big>'''The adsorption of light by a molecule is known as what?'''
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|a2=Fluorochromes
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||<font color="white"> <big>
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|l2=Immunofluorescence
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*'''''Excitation'''''
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|q3=What are the advantages of indirect staining?
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||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
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|a3=
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|-
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*Brighter fluorescence
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|<big>'''What are the fluorescent dyes commonly known as?'''
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*One preparation can be used to test many serum samples
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||<font color="white"> <big>
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*Relative expressions of different antigens in the same cell can be tested
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*'''''Fluorochromes'''''
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*Loss of antibody during conjugation is limited
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||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
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|l3=Immunofluorescence
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|-
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|q4=What technique is used to provide a quantitative measure of immunofluorescence?
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|<big>'''What are the advantages of indirect staining?'''
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|a4=Flow cytometry
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||<font color="white"> <big>
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|l4=Immunofluorescence
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*'''''Brighter fluorescence'''''
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</FlashCard>
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*'''''One preparation can be used to test many serum samples'''''
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*'''''Relative expressions of different antigens in the same cell can be tested'''''
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*'''''Loss of antibody during conjugation is limited'''''
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||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
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|-
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|<big>'''What technique is used to provide a quantitative measure of immunofluorescence?'''
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||<font color="white"> <big>
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*'''''Flow cytometry'''''
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||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
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|}
      
==<font color="purple">Radioimmunoassay</font>==
 
==<font color="purple">Radioimmunoassay</font>==
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{| border="3" cellpadding="8"
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<FlashCard questions="4">
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!width="400"|'''Question'''
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|q1=What are radioimmunoassays commonly used to detect?
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!width="400"|'''Answer'''
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|a2=
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!width="150"|'''Article'''
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*Hormone levels in blood and tissue fluids
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|-
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*Serum proteins
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|<big>'''What are radioimmunoassays commonly used to detect?'''
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*Drugs
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||<font color="white"> <big>
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*Vitamins
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*'''''Hormone levels in blood and tissue fluids'''''
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|l1=Radioimmunoassay
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*'''''Serum proteins'''''
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|q2=What is the most common isotope used for radioimmunoassay?
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*'''''Drugs'''''
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|a2=Iodine-125
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*'''''Vitamins'''''
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|l2=Radioimmunoassay
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||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
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|q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
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|-
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|a3=Ensures the number of epitopes present exceeds number of antibody-binding sites
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|<big>'''What is the most common isotope used for radioimmunoassay?'''
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|l3=Radioimmunoassay
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||<font color="white"> <big>
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|q4=What are the disadvantages of the radioimmunoassay?
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*'''''Iodine-125'''''
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|a4=
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||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
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*Substances used are radioactive
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|-
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*Gamma radiation requires special counting equipment
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|<big>'''Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?'''
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*Iodine naturally accumulates in the thyroid gland
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||<font color="white"> <big>
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|l4=Radioimmunoassay
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*'''''Ensures the number of epitopes present exceeds number of antibody-binding sites'''''
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</FlashCard>
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||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
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|-
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|<big>'''What are the disadvantages of the radioimmunoassay?'''
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||<font color="white"> <big>
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*'''''Substances used are radioactive'''''
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*'''''Gamma radiation requires special counting equipment'''''
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*'''''Iodine naturally accumulates in the thyroid gland'''''
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||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
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|}
   
==<font color="purple">Agglutination</font>==
 
==<font color="purple">Agglutination</font>==
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{| border="3" cellpadding="8"
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<FlashCard questions="5">
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!width="400"|'''Question'''
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|q1=What are the antibodies that produce agglutination known as?
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!width="400"|'''Answer'''
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|a1=Agglutinins
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!width="150"|'''Article'''
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|l1=Agglutination
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|-
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|q2=What constitutes the end of the agglutination test?
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|<big>'''What are the antibodies that produce agglutination known as?'''
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|a2=The last tube showing distinct agglutination
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||<font color="white"> <big>
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|l2=Agglutination
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*'''''Agglutinins'''''
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|q3=What does the titre represent?
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||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
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|a3=The number of antibodies per unit volume of serum
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|-
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|l3=Agglutination
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|<big>'''What constitutes the end of the agglutination test?'''
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|q4=What is the prozone effect?
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||<font color="white"> <big>
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|a4=An inhibition of agglutination caused by an excess of antibodies
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*'''''The last tube showing distinct agglutination'''''
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|l4=Agglutination
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||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
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|q5=What agglutination test is often used to test for rhesus disease?
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|-
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|a5=Coombs test
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|<big>'''What does the titre represent?'''
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|l5=Agglutination
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||<font color="white"> <big>
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</FlashCard>
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*'''''The number of antibodies per unit volume of serum'''''
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||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
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|-
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|<big>'''What is the prozone effect?'''
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||<font color="white"> <big>
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*'''''An inhibition of agglutination caused by an excess of antibodies'''''
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||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
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|-
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|<big>'''What agglutination test is often used to test for rhesus disease?'''
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||<font color="white"> <big>
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*'''''Coombs test'''''
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||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
     −
[[Category:Immunological Testing|Z]][[Category:Flashcards]]
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<br><br>
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{{Jim Bee 2007}}
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[[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]]
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