Difference between revisions of "In Vitro Fertilization - Anatomy & Physiology"
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Revision as of 11:59, 3 September 2008
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Oocyte Collection
- Donor is superovulated to provide large numbers of mature follicles.
- Pre-ovulatory follicles are collected by a surgical procedure involving aspiration of the follicular content.
- Oocytes captured in vitro.
- Direct follicle aspiration:
- Transvaginal Aspiration in the Mare:
- Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the rectum.
- Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.
- Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized.
- Hypodermic needle is advanced through the vaginal wall into the antral follicle.
- Follicular fluid containing the oocyte is aspirated under constant vacuum.
- Aspirated oocytes are placed in a culture vessel with capacitated spermatozoa.
- Could also be cultured in a 'nurse animal' in vivo.
- Embryos are incubated for a further period to the appropriate stage for transfer.
- Embryos transferred to recipient female.
- Can be transferred at the correct stage of the recipient's oestrous cycle.
- Embryos transferred around the blastocyst stage.
- Success rates are improving.
- Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations.
- Increases incidence of large offspring syndrome.