Changes

EP is often suspected if coughing pigs are present at the appropriate age.

EP is often suspected if coughing pigs are present at the appropriate age.

'''Culture and isolation''' are the gold standard but are difficult. See ''[[Mycoplasma hyopnumoniae]]'' for more details.  

'''Culture and isolation''' are the gold standard but are difficult. See ''[[Mycoplasma hyopneumoniae]]'' for more details.  

'''PCR''' can confirm the presence of the bacterium in the nasal cavity, <ref>Kurth, K. T., Hsu, T., Snook, E. R., Thacker, E. L., Thacker, B. J., Minion, F. C (2002) '''Use of a Mycoplasma hyopneumoniae nested polymerase chain reaction test to determine the optimal sampling sites in swine.''' J Vet Diagnostic Investigation, 14(6):463-469; 25</ref> or from '''lung tissue or bronchial washings'''. The latter two are the most reliable materials. Nested PCR requires very few organisms to be present for a positive result.

'''PCR''' can confirm the presence of the bacterium in the nasal cavity, <ref>Kurth, K. T., Hsu, T., Snook, E. R., Thacker, E. L., Thacker, B. J., Minion, F. C (2002) '''Use of a Mycoplasma hyopneumoniae nested polymerase chain reaction test to determine the optimal sampling sites in swine.''' J Vet Diagnostic Investigation, 14(6):463-469; 25</ref> or from '''lung tissue or bronchial washings'''. The latter two are the most reliable materials. Nested PCR requires very few organisms to be present for a positive result.