* Used to synchronize oestrus in groups of cows and heifers. Oestrus detection is difficult in this species, so this is advantageous for optimum timing of artificial insemination.
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* Give two injections of PGF2α or an analogue such as cloprostenol at an interval of 11 days to a group of cows/heifers all at different stages of the oestrous cycle.
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* 3-5 days after the second injection, all animals treated will come into oestrus and ovulate at about the same time.
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* If only a single injection is given, conception rates are lower after subsequent artificial insemination.
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* Two injections are required in order to ensure the prostaglandins are administered to all animals in a period where the corpus luteum is sensitive.
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* To reduce cost and improve pregnancy rates, the following regime is used:
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** All animals are injected with PGF2α on the same day and observed for oestrus during the following 5 days.
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** Any identified in oestrus are inseminated
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** Any not identified as being in oestrus receive a second injection of prostaglandin followed by artificial insemination.
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** Any animals that exhibit oestrus following the first insemination are reinseminated.