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Immunological Testing Flashcards (view source)
Revision as of 17:11, 17 March 2012
, 17:11, 17 March 2012→Agglutination
==<font color="purple">ELISA testing</font>==
==<font color="purple">ELISA testing</font>==
<FlashCard questions="3">
|q1=What does the acronym ELISA stand for?
|a1=Enzyme Linked ImmunoSorbent Assay
|l1=ELISA testing
|q2=What type of ELISA requires all the reagents to be added at once?
|a2=Homogenous
||<font color="white"> <big>
|l2=ELISA testing
|q3=What are the five basic steps of an ELISA test?
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
|a3=
*Adsorption of antigen/antibody onto solid phase
*Addition of chosen sample and reagents
||<font color="white"> <big>
*Incubation and washing
*Addition of enzyme-labelled antigen/antibody
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
*Addition of specific substrate
|l3=ELISA testing
</FlashCard>
||<font color="white"> <big>
*'''''Adsorption of antigen/antibody onto solid phase'''''
==<font color="purple">Immunofluorescence</font>==
*'''''Addition of chosen sample and reagents'''''
<FlashCard questions="4">
*'''''Incubation and washing'''''
|q1=The adsorption of light by a molecule is known as what?
*'''''Addition of enzyme-labelled antigen/antibody'''''
|a1=Excitation
*'''''Addition of specific substrate'''''
|l1=Immunofluorescence
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
|q2=What are the fluorescent dyes commonly known as?
|a2=Fluorochromes
|l2=Immunofluorescence
|q3=What are the advantages of indirect staining?
|a3=
*Brighter fluorescence
*One preparation can be used to test many serum samples
*Relative expressions of different antigens in the same cell can be tested
*Loss of antibody during conjugation is limited
|l3=Immunofluorescence
|q4=What technique is used to provide a quantitative measure of immunofluorescence?
|a4=Flow cytometry
|l4=Immunofluorescence
</FlashCard>
==<font color="purple">Radioimmunoassay</font>==
<FlashCard questions="4">
|q1=What are radioimmunoassays commonly used to detect?
|a2=
*Hormone levels in blood and tissue fluids
*Serum proteins
*Drugs
*Vitamins
|l1=Radioimmunoassay
|q2=What is the most common isotope used for radioimmunoassay?
|a2=Iodine-125
|l2=Radioimmunoassay
|q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
|a3=Ensures the number of epitopes present exceeds number of antibody-binding sites
|l3=Radioimmunoassay
|q4=What are the disadvantages of the radioimmunoassay?
|a4=
*Substances used are radioactive
*Gamma radiation requires special counting equipment
*Iodine naturally accumulates in the thyroid gland
|l4=Radioimmunoassay
</FlashCard>
==<font color="purple">Agglutination</font>==
<FlashCard questions="5">
|q1=What are the antibodies that produce agglutination known as?
|a1=Agglutinins
|l1=Agglutination
|q2=What constitutes the end of the agglutination test?
|a2=The last tube showing distinct agglutination
|l2=Agglutination
|q3=What does the titre represent?
|a3=The number of antibodies per unit volume of serum
|l3=Agglutination
|q4=What is the prozone effect?
|a4=An inhibition of agglutination caused by an excess of antibodies
|l4=Agglutination
|q5=What agglutination test is often used to test for rhesus disease?
|a5=Coombs test
|l5=Agglutination
</FlashCard>
<br><br>
{{Jim Bee 2007}}
[[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]]