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Immunological Testing Flashcards (view source)
Revision as of 11:31, 15 September 2008
, 11:31, 15 September 2008no edit summary
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*'''''Excitation'''''
*'''''Excitation'''''
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
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|<big>'''What are the fluorescent dyes commonly known as?'''
|<big>'''What are the fluorescent dyes commonly known as?'''
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*'''''Fluorochromes'''''
*'''''Fluorochromes'''''
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
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|<big>'''What are the advantages of indirect staining?'''
|<big>'''What are the advantages of indirect staining?'''
*'''''Relative expressions of different antigens in the same cell can be tested'''''
*'''''Relative expressions of different antigens in the same cell can be tested'''''
*'''''Loss of antibody during conjugation is limited'''''
*'''''Loss of antibody during conjugation is limited'''''
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
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|<big>'''What technique is used to provide a quantitative measure of immunofluorescence?'''
|<big>'''What technique is used to provide a quantitative measure of immunofluorescence?'''
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||<font color="white"> <big>
*'''''Flow cytometry'''''
*'''''Flow cytometry'''''
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
|}
==<font color="purple">Radioimmunoassay</font>==
{| border="3" cellpadding="8"
!width="400"|'''Question'''
!width="400"|'''Answer'''
!width="150"|'''Article'''
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|<big>'''What are radioimmunoassays commonly used to detect?'''
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*'''''Hormone levels in blood and tissue fluids'''''
*'''''Serum proteins'''''
*'''''Drugs'''''
*'''''Vitamins'''''
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
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|<big>'''What is the most common isotope used for radioimmunoassay?'''
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*'''''Iodine-125'''''
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
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|<big>'''Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?'''
||<font color="white"> <big>
*'''''Ensures the number of epitopes present exceeds number of antibody-binding sites'''''
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
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|<big>'''What are the disadvantages of the radioimmunoassay?'''
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*'''''Substances used are radioactive'''''
*'''''Gamma radiation requires special counting equipment'''''
*'''''Iodine naturally accumulates in the thyroid gland'''''
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
|}
==<font color="purple">Agglutination</font>==
{| border="3" cellpadding="8"
!width="400"|'''Question'''
!width="400"|'''Answer'''
!width="150"|'''Article'''
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|<big>'''What are the antibodies that produce agglutination known as?'''
||<font color="white"> <big>
*'''''Agglutinins'''''
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
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|<big>'''What constitutes the end of the agglutination test?'''
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*'''''The last tube showing distinct agglutination'''''
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
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|<big>'''What does the titre represent?'''
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*'''''The number of antibodies per unit volume of serum'''''
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
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|<big>'''What is the prozone effect?'''
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*'''''An inhibition of agglutination caused by an excess of antibodies'''''
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
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|<big>'''What agglutination test is often used to test for rhesus disease?'''
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*'''''Coombs test'''''
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]