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→Fibrin Degradation Products
===Fibrin Degradation Products===
===Fibrin Degradation Products===
A latex agglutination test is available for fibrin degradation products (FDP). To perform the test, serial dilutions of test s are prepared and Anti-FDP antibodies adsorbed onto latex particles are added. At a dilution, the test is positive if agglutination is seen, and the most dilute sample that agglutinates gives the overall result of the test. Normal values are between 1/4 and 1/16.
A latex agglutination test is available for fibrin degradation products (FDP). To perform the test, anti-FDP antibodies adsorbed onto latex particles are added to serial dilutions of test serum. If agglutination is seen at a particular dilution, the test is positive. The most dilute sample that agglutinates gives the overall result of the test. Normal values are between 1/4 and 1/16.
Although the test is simple to perform, interpretation may be challenging. The action of thrombin on fibrinogen is to cleave the protein and produce smaller compounds called fibrinopeptides, plus the fibrin monomer. This monomer polymerizes to form the fibrin gel. The gel is stabilized by the action of factor XIII, activated by thrombin.
Although well standardized and easy to perform, the FSP value may be difficult to interpret. The action of thrombin on fibrinogen is to cleave the protein and produce smaller compounds called fibrinopeptides, plus the fibrin monomer. This monomer polymerizes to form the fibrin gel. The gel is stabilized by the action of factor XIII, activated by thrombin.
The action of plasmin is to cleave both fibrinogen and fibrin. Its action is localized by its activation at the site of endothelial rupture, and the tight association of plasminogen and fibrin. The activity on fibrinogen forms small fragments, D and E. The action on fibrin polymer is to form larger fragments. These fragments are anticoagulants formed at the site of coagulation and serve to inhibit the action of thrombin on fibrinogen to form fibrin. Both are also measured by the technique described above.
The action of plasmin is to cleave both fibrinogen and fibrin. Its action is localized by its activation at the site of endothelial rupture, and the tight association of plasminogen and fibrin. The activity on fibrinogen forms small fragments, D and E. The action on fibrin polymer is to form larger fragments. These fragments are anticoagulants formed at the site of coagulation and serve to inhibit the action of thrombin on fibrinogen to form fibrin. Both are also measured by the technique described above.