Difference between revisions of "Immunological Testing Flashcards"
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==<font color="purple">Radioimmunoassay</font>== | ==<font color="purple">Radioimmunoassay</font>== | ||
− | + | {| border="3" cellpadding="8" | |
− | | | + | !width="400"|'''Question''' |
− | | | + | !width="400"|'''Answer''' |
− | *Hormone levels in blood and tissue fluids | + | !width="150"|'''Article''' |
− | *Serum proteins | + | |- |
− | *Drugs | + | |<big>'''What are radioimmunoassays commonly used to detect?''' |
− | *Vitamins | + | ||<font color="white"> <big> |
− | | | + | *'''''Hormone levels in blood and tissue fluids''''' |
− | | | + | *'''''Serum proteins''''' |
− | | | + | *'''''Drugs''''' |
− | | | + | *'''''Vitamins''''' |
− | | | + | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] |
− | | | + | |- |
− | | | + | |<big>'''What is the most common isotope used for radioimmunoassay?''' |
− | | | + | ||<font color="white"> <big> |
− | | | + | *'''''Iodine-125''''' |
− | *Substances used are radioactive | + | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] |
− | *Gamma radiation requires special counting equipment | + | |- |
− | *Iodine naturally accumulates in the thyroid gland | + | |<big>'''Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?''' |
− | | | + | ||<font color="white"> <big> |
− | </ | + | *'''''Ensures the number of epitopes present exceeds number of antibody-binding sites''''' |
+ | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] | ||
+ | |- | ||
+ | |<big>'''What are the disadvantages of the radioimmunoassay?''' | ||
+ | ||<font color="white"> <big> | ||
+ | *'''''Substances used are radioactive''''' | ||
+ | *'''''Gamma radiation requires special counting equipment''''' | ||
+ | *'''''Iodine naturally accumulates in the thyroid gland''''' | ||
+ | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] | ||
+ | |} | ||
==<font color="purple">Agglutination</font>== | ==<font color="purple">Agglutination</font>== | ||
− | + | {| border="3" cellpadding="8" | |
− | | | + | !width="400"|'''Question''' |
− | | | + | !width="400"|'''Answer''' |
− | | | + | !width="150"|'''Article''' |
− | | | + | |- |
− | | | + | |<big>'''What are the antibodies that produce agglutination known as?''' |
− | | | + | ||<font color="white"> <big> |
− | | | + | *'''''Agglutinins''''' |
− | | | + | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] |
− | | | + | |- |
− | | | + | |<big>'''What constitutes the end of the agglutination test?''' |
− | | | + | ||<font color="white"> <big> |
− | | | + | *'''''The last tube showing distinct agglutination''''' |
− | | | + | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] |
− | | | + | |- |
− | | | + | |<big>'''What does the titre represent?''' |
− | </ | + | ||<font color="white"> <big> |
+ | *'''''The number of antibodies per unit volume of serum''''' | ||
+ | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] | ||
+ | |- | ||
+ | |<big>'''What is the prozone effect?''' | ||
+ | ||<font color="white"> <big> | ||
+ | *'''''An inhibition of agglutination caused by an excess of antibodies''''' | ||
+ | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] | ||
+ | |- | ||
+ | |<big>'''What agglutination test is often used to test for rhesus disease?''' | ||
+ | ||<font color="white"> <big> | ||
+ | *'''''Coombs test''''' | ||
+ | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] | ||
Revision as of 17:04, 17 March 2012
ELISA testing
Question | Answer | Article | |
What does the acronym ELISA stand for? | Enzyme Linked ImmunoSorbent Assay
|
Link to Article | |
What type of ELISA requires all the reagents to be added at once? | Homogenous
|
Link to Article | |
What are the five basic steps of an ELISA test? |
|
Link to Article |
Immunofluorescence
Question | Answer | Article | |
The adsorption of light by a molecule is known as what? | Excitation
|
Link to Article | |
What are the fluorescent dyes commonly known as? | Fluorochromes
|
Link to Article | |
What are the advantages of indirect staining? |
|
Link to Article | |
What technique is used to provide a quantitative measure of immunofluorescence? | Flow cytometry
|
Link to Article |
Radioimmunoassay
Question | Answer | Article |
---|---|---|
What are radioimmunoassays commonly used to detect? |
|
Link to Answer Article |
What is the most common isotope used for radioimmunoassay? |
|
Link to Answer Article |
Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? |
|
Link to Answer Article |
What are the disadvantages of the radioimmunoassay? |
|
Link to Answer Article |
Agglutination
Question | Answer | Article | ||
---|---|---|---|---|
What are the antibodies that produce agglutination known as? |
|
Link to Answer Article | ||
What constitutes the end of the agglutination test? |
|
Link to Answer Article | ||
What does the titre represent? |
|
Link to Answer Article | ||
What is the prozone effect? |
|
Link to Answer Article | ||
What agglutination test is often used to test for rhesus disease? |
|
Link to Answer Article
|