Difference between revisions of "Immunological Testing Flashcards"

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==<font color="purple">ELISA testing</font>==
 
==<font color="purple">ELISA testing</font>==
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{| border="3" cellpadding="8"
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<FlashCard questions="3">
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!width="400"|'''Question'''
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|q1=What does the acronym ELISA stand for?
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!width="400"|'''Answer'''
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|a1=Enzyme Linked ImmunoSorbent Assay
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!width="150"|'''Article'''
+
|l1=ELISA testing
−
|-
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|q2=What type of ELISA requires all the reagents to be added at once?
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|<big>'''What does the acronym ELISA stand for?'''
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|a2=Homogenous
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||<font color="white"> <big>
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|l2=ELISA testing
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*'''''Enzyme Linked ImmunoSorbent Assay'''''
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|q3=What are the five basic steps of an ELISA test?
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||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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|a3=
−
|-
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*Adsorption of antigen/antibody onto solid phase
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|<big>'''What type of ELISA requires all the reagents to be added at once?'''
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*Addition of chosen sample and reagents
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||<font color="white"> <big>
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*Incubation and washing
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*'''''Homogenous'''''
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*Addition of enzyme-labelled antigen/antibody
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||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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*Addition of specific substrate
−
|-
+
|l3=ELISA testing
−
|<big>'''What are the five basic steps of an ELISA test?'''
+
</FlashCard>
−
||<font color="white"> <big>
 
−
*'''''Adsorption of antigen/antibody onto solid phase'''''
 
−
*'''''Addition of chosen sample and reagents'''''
 
−
*'''''Incubation and washing'''''
 
−
*'''''Addition of enzyme-labelled antigen/antibody'''''
 
−
*'''''Addition of specific substrate'''''
 
−
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
 
  
−
|}
 
 
==<font color="purple">Immunofluorescence</font>==
 
==<font color="purple">Immunofluorescence</font>==
−
{| border="3" cellpadding="8"
+
<FlashCard questions="4">
−
!width="400"|'''Question'''
+
|q1=The adsorption of light by a molecule is known as what?
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!width="400"|'''Answer'''
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|a1=Excitation
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!width="150"|'''Article'''
+
|l1=Immunofluorescence
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|-
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|q2=What are the fluorescent dyes commonly known as?
−
|<big>'''The adsorption of light by a molecule is known as what?'''
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|a2=Fluorochromes
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||<font color="white"> <big>
+
|l2=Immunofluorescence
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*'''''Excitation'''''
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|q3=What are the advantages of indirect staining?
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||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
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|a3=
−
|-
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*Brighter fluorescence
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|<big>'''What are the fluorescent dyes commonly known as?'''
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*One preparation can be used to test many serum samples
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||<font color="white"> <big>
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*Relative expressions of different antigens in the same cell can be tested
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*'''''Fluorochromes'''''
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*Loss of antibody during conjugation is limited
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||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
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|l3=Immunofluorescence
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|-
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|q4=What technique is used to provide a quantitative measure of immunofluorescence?
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|<big>'''What are the advantages of indirect staining?'''
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|a4=Flow cytometry
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||<font color="white"> <big>
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|l4=Immunofluorescence
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*'''''Brighter fluorescence'''''
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</FlashCard>
−
*'''''One preparation can be used to test many serum samples'''''
 
−
*'''''Relative expressions of different antigens in the same cell can be tested'''''
 
−
*'''''Loss of antibody during conjugation is limited'''''
 
−
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
 
−
|-
 
−
|<big>'''What technique is used to provide a quantitative measure of immunofluorescence?'''
 
−
||<font color="white"> <big>
 
−
*'''''Flow cytometry'''''
 
−
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
 
−
|}
 
  
 
==<font color="purple">Radioimmunoassay</font>==
 
==<font color="purple">Radioimmunoassay</font>==
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{| border="3" cellpadding="8"
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<FlashCard questions="4">
−
!width="400"|'''Question'''
+
|q1=What are radioimmunoassays commonly used to detect?
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!width="400"|'''Answer'''
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|a2=
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!width="150"|'''Article'''
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*Hormone levels in blood and tissue fluids
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|-
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*Serum proteins
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|<big>'''What are radioimmunoassays commonly used to detect?'''
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*Drugs
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||<font color="white"> <big>
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*Vitamins
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*'''''Hormone levels in blood and tissue fluids'''''
+
|l1=Radioimmunoassay
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*'''''Serum proteins'''''
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|q2=What is the most common isotope used for radioimmunoassay?
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*'''''Drugs'''''
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|a2=Iodine-125
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*'''''Vitamins'''''
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|l2=Radioimmunoassay
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||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
+
|q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
−
|-
+
|a3=Ensures the number of epitopes present exceeds number of antibody-binding sites
−
|<big>'''What is the most common isotope used for radioimmunoassay?'''
+
|l3=Radioimmunoassay
−
||<font color="white"> <big>
+
|q4=What are the disadvantages of the radioimmunoassay?
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*'''''Iodine-125'''''
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|a4=
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||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
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*Substances used are radioactive
−
|-
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*Gamma radiation requires special counting equipment
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|<big>'''Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?'''
+
*Iodine naturally accumulates in the thyroid gland
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||<font color="white"> <big>
+
|l4=Radioimmunoassay
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*'''''Ensures the number of epitopes present exceeds number of antibody-binding sites'''''
+
</FlashCard>
−
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
 
−
|-
 
−
|<big>'''What are the disadvantages of the radioimmunoassay?'''
 
−
||<font color="white"> <big>
 
−
*'''''Substances used are radioactive'''''
 
−
*'''''Gamma radiation requires special counting equipment'''''
 
−
*'''''Iodine naturally accumulates in the thyroid gland'''''
 
−
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
 
  
−
|}
 
 
==<font color="purple">Agglutination</font>==
 
==<font color="purple">Agglutination</font>==
−
{| border="3" cellpadding="8"
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<FlashCard questions="5">
−
!width="400"|'''Question'''
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|q1=What are the antibodies that produce agglutination known as?
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!width="400"|'''Answer'''
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|a1=Agglutinins
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!width="150"|'''Article'''
+
|l1=Agglutination
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|-
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|q2=What constitutes the end of the agglutination test?
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|<big>'''What are the antibodies that produce agglutination known as?'''
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|a2=The last tube showing distinct agglutination
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||<font color="white"> <big>
+
|l2=Agglutination
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*'''''Agglutinins'''''
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|q3=What does the titre represent?
−
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
+
|a3=The number of antibodies per unit volume of serum
−
|-
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|l3=Agglutination
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|<big>'''What constitutes the end of the agglutination test?'''
+
|q4=What is the prozone effect?
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||<font color="white"> <big>
+
|a4=An inhibition of agglutination caused by an excess of antibodies
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*'''''The last tube showing distinct agglutination'''''
+
|l4=Agglutination
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||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
+
|q5=What agglutination test is often used to test for rhesus disease?
−
|-
+
|a5=Coombs test
−
|<big>'''What does the titre represent?'''
+
|l5=Agglutination
−
||<font color="white"> <big>
+
</FlashCard>
−
*'''''The number of antibodies per unit volume of serum'''''
 
−
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 
−
|-
 
−
|<big>'''What is the prozone effect?'''
 
−
||<font color="white"> <big>
 
−
*'''''An inhibition of agglutination caused by an excess of antibodies'''''
 
−
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 
−
|-
 
−
|<big>'''What agglutination test is often used to test for rhesus disease?'''
 
−
||<font color="white"> <big>
 
−
*'''''Coombs test'''''
 
−
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 
  
 +
 +
<br><br>
 +
{{Jim Bee 2007}}
 
[[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]]
 
[[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]]

Latest revision as of 17:11, 17 March 2012

ELISA testing

Question Answer Article
What does the acronym ELISA stand for? Link to Article
What type of ELISA requires all the reagents to be added at once? Link to Article
What are the five basic steps of an ELISA test? Link to Article


Immunofluorescence

Question Answer Article
The adsorption of light by a molecule is known as what? Link to Article
What are the fluorescent dyes commonly known as? Link to Article
What are the advantages of indirect staining? Link to Article
What technique is used to provide a quantitative measure of immunofluorescence? Link to Article


Radioimmunoassay

Question Answer Article
What are radioimmunoassays commonly used to detect? Link to Article
What is the most common isotope used for radioimmunoassay? Link to Article
Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? Link to Article
What are the disadvantages of the radioimmunoassay? Link to Article


Agglutination

Question Answer Article
What are the antibodies that produce agglutination known as? Link to Article
What constitutes the end of the agglutination test? Link to Article
What does the titre represent? Link to Article
What is the prozone effect? Link to Article
What agglutination test is often used to test for rhesus disease? Link to Article





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