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Semen Sexing - Anatomy & Physiology (view source)
Revision as of 10:19, 17 July 2008
, 10:19, 17 July 2008→Flow Cytometry
== Flow Cytometry ==
== Flow Cytometry ==
* Ejaculated spermatozoa are treated with a DNA dye (fluorochrome).
* X-bearing sperm absorb more dye than Y-bearing sperm.
* X-bearing sperm thus emit more intense light when excited by a laser.
* Sperm are also treated with a dye that greatly suppresses the signal from dead sperm.
** Dead sperm are therefore identified and rejected.
* Once spermatozoa enter the flow cytometer chamber