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| | + | {{toplink |
| | + | |backcolour = f5fffa |
| | + | |linkpage =Immunological testing - WikiBlood |
| | + | |linktext =IMMUNOLOGICAL TESTING |
| | + | |sublink1 =Flash Cards - WikiBlood |
| | + | |subtext1 =WIKIBLOOD FLASHCARDS |
| | + | |pagetype =Blood |
| | + | }} |
| | + | <br> |
| | ==<font color="purple">ELISA testing</font>== | | ==<font color="purple">ELISA testing</font>== |
| − | <FlashCard questions="3">
| + | {| border="3" cellpadding="8" |
| − | |q1=What does the acronym ELISA stand for? | + | !width="400"|'''Question''' |
| − | |a1=Enzyme Linked ImmunoSorbent Assay
| + | !width="400"|'''Answer''' |
| − | |l1=ELISA testing | + | !width="150"|'''Article''' |
| − | |q2=What type of ELISA requires all the reagents to be added at once?
| + | |- |
| − | |a2=Homogenous | + | |<big>'''What does the acronym ELISA stand for?''' |
| − | |l2=ELISA testing | + | ||<font color="white"> <big> |
| − | |q3=What are the five basic steps of an ELISA test? | + | *'''''Enzyme Linked ImmunoSorbent Assay''''' |
| − | |a3= | + | ||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]] |
| − | *Adsorption of antigen/antibody onto solid phase
| + | |- |
| − | *Addition of chosen sample and reagents
| + | |<big>'''What type of ELISA requires all the reagents to be added at once?''' |
| − | *Incubation and washing
| + | ||<font color="white"> <big> |
| − | *Addition of enzyme-labelled antigen/antibody
| + | *'''''Homogenous''''' |
| − | *Addition of specific substrate
| + | ||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]] |
| − | |l3=ELISA testing | |
| − | </FlashCard>
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| − | | |
| − | ==<font color="purple">Immunofluorescence</font>==
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| − | <FlashCard questions="4">
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| − | |q1=The adsorption of light by a molecule is known as what?
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| − | |a1=Excitation
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| − | |l1=Immunofluorescence
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| − | |q2=What are the fluorescent dyes commonly known as?
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| − | |a2=Fluorochromes
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| − | |l2=Immunofluorescence
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| − | |q3=What are the advantages of indirect staining?
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| − | |a3=
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| − | *Brighter fluorescence | |
| − | *One preparation can be used to test many serum samples
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| − | *Relative expressions of different antigens in the same cell can be tested
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| − | *Loss of antibody during conjugation is limited
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| − | |l3=Immunofluorescence | |
| − | |q4=What technique is used to provide a quantitative measure of immunofluorescence? | |
| − | |a4=Flow cytometry
| |
| − | |l4=Immunofluorescence | |
| − | </FlashCard> | |
| − | | |
| − | ==<font color="purple">Radioimmunoassay</font>==
| |
| − | <FlashCard questions="4">
| |
| − | |q1=What are radioimmunoassays commonly used to detect?
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| − | |a2=
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| − | *Hormone levels in blood and tissue fluids
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| − | *Serum proteins
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| − | *Drugs
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| − | *Vitamins
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| − | |l1=Radioimmunoassay
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| − | |q2=What is the most common isotope used for radioimmunoassay? | |
| − | |a2=Iodine-125
| |
| − | |l2=Radioimmunoassay | |
| − | |q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
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| − | |a3=Ensures the number of epitopes present exceeds number of antibody-binding sites
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| − | |l3=Radioimmunoassay
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| − | |q4=What are the disadvantages of the radioimmunoassay?
| |
| − | |a4= | |
| − | *Substances used are radioactive
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| − | *Gamma radiation requires special counting equipment
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| − | *Iodine naturally accumulates in the thyroid gland
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| − | |l4=Radioimmunoassay | |
| − | </FlashCard>
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| − | | |
| − | ==<font color="purple">Agglutination</font>==
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| − | <FlashCard questions="5"> | |
| − | |q1=What are the antibodies that produce agglutination known as?
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| − | |a1=Agglutinins
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| − | |l1=Agglutination
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| − | |q2=What constitutes the end of the agglutination test?
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| − | |a2=The last tube showing distinct agglutination
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| − | |l2=Agglutination
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| − | |q3=What does the titre represent?
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| − | |a3=The number of antibodies per unit volume of serum
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| − | |l3=Agglutination
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| − | |q4=What is the prozone effect?
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| − | |a4=An inhibition of agglutination caused by an excess of antibodies
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| − | |l4=Agglutination
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| − | |q5=What agglutination test is often used to test for rhesus disease?
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| − | |a5=Coombs test
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| − | |l5=Agglutination
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| − | </FlashCard> | |
| − | | |
| − | | |
| − | <br><br>
| |
| − | {{Jim Bee 2007}}
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| − | [[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]]
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