Difference between revisions of "Immunological Testing Flashcards"

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|linkpage =Immunological testing - WikiBlood
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|linktext =IMMUNOLOGICAL TESTING
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|sublink1 =Flash Cards - WikiBlood
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|subtext1 =WIKIBLOOD FLASHCARDS
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|pagetype =Blood
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<br>
 
==<font color="purple">ELISA testing</font>==
 
==<font color="purple">ELISA testing</font>==
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<FlashCard questions="3">
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{| border="3" cellpadding="8"
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|q1=What does the acronym ELISA stand for?
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!width="400"|'''Question'''
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|a1=Enzyme Linked ImmunoSorbent Assay
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!width="400"|'''Answer'''
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|l1=ELISA testing
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!width="150"|'''Article'''
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|q2=What type of ELISA requires all the reagents to be added at once?
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|-
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|a2=Homogenous
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|<big>'''What does the acronym ELISA stand for?'''
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|l2=ELISA testing
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||<font color="white"> <big>
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|q3=What are the five basic steps of an ELISA test?
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*'''''Enzyme Linked ImmunoSorbent Assay'''''
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|a3=
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||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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*Adsorption of antigen/antibody onto solid phase
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|-
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*Addition of chosen sample and reagents
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|<big>'''What type of ELISA requires all the reagents to be added at once?'''
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*Incubation and washing
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||<font color="white"> <big>
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*Addition of enzyme-labelled antigen/antibody
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*'''''Homogenous'''''
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*Addition of specific substrate
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||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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|l3=ELISA testing
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|-
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</FlashCard>
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|<big>'''What are the five basic steps of an ELISA test?'''
 +
||<font color="white"> <big>
 +
*'''''Adsorption of antigen/antibody onto solid phase'''''
 +
*'''''Addition of chosen sample and reagents'''''
 +
*'''''Incubation and washing'''''
 +
*'''''Addition of enzyme-labelled antigen/antibody'''''
 +
*'''''Addition of specific substrate'''''
 +
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
  
 +
|}
 
==<font color="purple">Immunofluorescence</font>==
 
==<font color="purple">Immunofluorescence</font>==
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<FlashCard questions="4">
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{| border="3" cellpadding="8"
−
|q1=The adsorption of light by a molecule is known as what?
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!width="400"|'''Question'''
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|a1=Excitation
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!width="400"|'''Answer'''
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|l1=Immunofluorescence
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!width="150"|'''Article'''
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|q2=What are the fluorescent dyes commonly known as?
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|-
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|a2=Fluorochromes
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|<big>'''The adsorption of light by a molecule is known as what?'''
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|l2=Immunofluorescence
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||<font color="white"> <big>
−
|q3=What are the advantages of indirect staining?
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*'''''Excitation'''''
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|a3=
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||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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*Brighter fluorescence
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|-
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*One preparation can be used to test many serum samples
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|<big>'''What are the fluorescent dyes commonly known as?'''
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*Relative expressions of different antigens in the same cell can be tested
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||<font color="white"> <big>
−
*Loss of antibody during conjugation is limited
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*'''''Fluorochromes'''''
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|l3=Immunofluorescence
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||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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|q4=What technique is used to provide a quantitative measure of immunofluorescence?
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|-
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|a4=Flow cytometry
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|<big>'''What are the advantages of indirect staining?'''
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|l4=Immunofluorescence
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||<font color="white"> <big>
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</FlashCard>
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*'''''Brighter fluorescence'''''
−
 
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*'''''One preparation can be used to test many serum samples'''''
−
==<font color="purple">Radioimmunoassay</font>==
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*'''''Relative expressions of different antigens in the same cell can be tested'''''
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<FlashCard questions="4">
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*'''''Loss of antibody during conjugation is limited'''''
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|q1=What are radioimmunoassays commonly used to detect?
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||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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|a2=
 
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*Hormone levels in blood and tissue fluids
 
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*Serum proteins
 
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*Drugs
 
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*Vitamins
 
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|l1=Radioimmunoassay
 
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|q2=What is the most common isotope used for radioimmunoassay?
 
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|a2=Iodine-125
 
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|l2=Radioimmunoassay
 
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|q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
 
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|a3=Ensures the number of epitopes present exceeds number of antibody-binding sites
 
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|l3=Radioimmunoassay
 
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|q4=What are the disadvantages of the radioimmunoassay?
 
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|a4=
 
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*Substances used are radioactive
 
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*Gamma radiation requires special counting equipment
 
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*Iodine naturally accumulates in the thyroid gland
 
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|l4=Radioimmunoassay
 
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</FlashCard>
 
−
 
 
−
==<font color="purple">Agglutination</font>==
 
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<FlashCard questions="5">
 
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|q1=What are the antibodies that produce agglutination known as?
 
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|a1=Agglutinins
 
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|l1=Agglutination
 
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|q2=What constitutes the end of the agglutination test?
 
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|a2=The last tube showing distinct agglutination
 
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|l2=Agglutination
 
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|q3=What does the titre represent?
 
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|a3=The number of antibodies per unit volume of serum
 
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|l3=Agglutination
 
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|q4=What is the prozone effect?
 
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|a4=An inhibition of agglutination caused by an excess of antibodies
 
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|l4=Agglutination
 
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|q5=What agglutination test is often used to test for rhesus disease?
 
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|a5=Coombs test
 
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|l5=Agglutination
 
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</FlashCard>
 
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<br><br>
 
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{{Jim Bee 2007}}
 
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[[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]]
 

Revision as of 10:59, 15 September 2008

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IMMUNOLOGICAL TESTING
WIKIBLOOD FLASHCARDS



ELISA testing

Question Answer Article
What does the acronym ELISA stand for?
  • Enzyme Linked ImmunoSorbent Assay
 Link to Answer Article
What type of ELISA requires all the reagents to be added at once?
  • Homogenous
 Link to Answer Article
What are the five basic steps of an ELISA test?
  • Adsorption of antigen/antibody onto solid phase
  • Addition of chosen sample and reagents
  • Incubation and washing
  • Addition of enzyme-labelled antigen/antibody
  • Addition of specific substrate
 Link to Answer Article

Immunofluorescence

Question Answer Article
The adsorption of light by a molecule is known as what?
  • Excitation
 Link to Answer Article
What are the fluorescent dyes commonly known as?
  • Fluorochromes
 Link to Answer Article
What are the advantages of indirect staining?
  • Brighter fluorescence
  • One preparation can be used to test many serum samples
  • Relative expressions of different antigens in the same cell can be tested
  • Loss of antibody during conjugation is limited
 Link to Answer Article
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