Difference between revisions of "In Vitro Fertilization - Anatomy & Physiology"
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m (Text replace - "[[Female_Reproductive_Tract_-Oestrous_Cycle_- Anatomy & Physiology" to "[[Oestrous Cycle - Anatomy & Physiology") |
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+ | {{toplink | ||
+ | |backcolour =EED2EE | ||
+ | |linkpage =Reproductive System - Anatomy & Physiology | ||
+ | |linktext =Reproductive System | ||
+ | |maplink = Reproductive System (Content Map) - Anatomy & Physiology | ||
+ | |sublink1=Reproductive System - Anatomy & Physiology#Reproductive Technologies | ||
+ | |subtext1=REPRODUCTIVE TECHNOLOGIES | ||
+ | }} | ||
+ | <br> | ||
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== Oocyte Collection== | == Oocyte Collection== | ||
− | * Donor is superovulated to provide large numbers of mature [[ | + | * Donor is superovulated to provide large numbers of mature [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]]. |
− | * Pre-ovulatory [[ | + | * Pre-ovulatory [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]] are collected by a surgical procedure involving aspiration of the follicular content. |
− | * [[ | + | * [[The_Ovary_-_Oocytes_- Anatomy & Physiology|Oocytes]] captured in vitro. |
− | * Direct [[ | + | * Direct [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]] aspiration: |
− | ** A hyperdermic needle is inserted into the [[ | + | ** A hyperdermic needle is inserted into the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]]. |
** Follicular fluid is aspirated. | ** Follicular fluid is aspirated. | ||
− | ** Follicular fluid forcefully returned to the [[ | + | ** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]]. |
− | ** Repeated 2-3 times to dislodge [[ | + | ** Repeated 2-3 times to dislodge [[The_Ovary_-_Oocytes_- Anatomy & Physiology|oocytes]]. |
− | + | ||
* Transvaginal Aspiration in the Mare: | * Transvaginal Aspiration in the Mare: | ||
** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_- Anatomy & Physiology|rectum]]. | ** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_- Anatomy & Physiology|rectum]]. | ||
** Lubricated ultrasound transducer is inserted into the [[Vagina and Vestibule - Anatomy & Physiology|vagina]] and held in the fornix vagina. | ** Lubricated ultrasound transducer is inserted into the [[Vagina and Vestibule - Anatomy & Physiology|vagina]] and held in the fornix vagina. | ||
− | ** [[ | + | ** [[Female_Reproductive_Tract_-The_Ovary_- Anatomy & Physiology|Ovary]] transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]] can be visualized. |
** Hypodermic needle is advanced through the [[Vagina and Vestibule - Anatomy & Physiology|vaginal wall]] into the antral follicle. | ** Hypodermic needle is advanced through the [[Vagina and Vestibule - Anatomy & Physiology|vaginal wall]] into the antral follicle. | ||
− | ** Follicular fluid containing the [[ | + | ** Follicular fluid containing the [[The_Ovary_-_Oocytes_- Anatomy & Physiology|oocyte]] is aspirated under constant vacuum. |
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− | + | * Aspirated [[The_Ovary_-_Oocytes_- Anatomy & Physiology|oocytes]] are placed in a culture vessel with [[Copulation_-Sperm_in_the_Female_Tract_- Anatomy & Physiology#Capacitation|capacitated]] spermatozoa. | |
+ | ** Could also be cultured in a 'nurse animal' in vivo. | ||
* Embryos are incubated for a further period to the appropriate stage for transfer. | * Embryos are incubated for a further period to the appropriate stage for transfer. | ||
* Embryos transferred to recipient female. | * Embryos transferred to recipient female. | ||
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* Embryos transferred around the [[Blastocyst Embryonic Development - Anatomy & Physiology|blastocyst]] stage. | * Embryos transferred around the [[Blastocyst Embryonic Development - Anatomy & Physiology|blastocyst]] stage. | ||
* Success rates are improving. | * Success rates are improving. | ||
− | * Embryos can be frozen, eliminating the need for tight | + | * Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations. |
− | * Increases incidence of [[Large_Offspring|large offspring syndrome]]. | + | * Increases incidence of [[Parturition_-_Risks_in_Parturition_- Anatomy & Physiology#Large_Offspring|large offspring syndrome]]. |
− | [[Category:Reproductive | + | [[Category:Reproductive System]] |
− |
Revision as of 22:39, 30 November 2010
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Oocyte Collection
- Donor is superovulated to provide large numbers of mature follicles.
- Pre-ovulatory follicles are collected by a surgical procedure involving aspiration of the follicular content.
- Oocytes captured in vitro.
- Direct follicle aspiration:
- Transvaginal Aspiration in the Mare:
- Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the rectum.
- Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.
- Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized.
- Hypodermic needle is advanced through the vaginal wall into the antral follicle.
- Follicular fluid containing the oocyte is aspirated under constant vacuum.
- Aspirated oocytes are placed in a culture vessel with capacitated spermatozoa.
- Could also be cultured in a 'nurse animal' in vivo.
- Embryos are incubated for a further period to the appropriate stage for transfer.
- Embryos transferred to recipient female.
- Can be transferred at the correct stage of the recipient's oestrous cycle.
- Embryos transferred around the blastocyst stage.
- Success rates are improving.
- Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations.
- Increases incidence of large offspring syndrome.