Difference between revisions of "Immunodiffusion"

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==Introduction==
 
Immunodiffusion reactions are diagnostic tests that use the diffusion of particles through a medium (such as agar) to measure:
 
Immunodiffusion reactions are diagnostic tests that use the diffusion of particles through a medium (such as agar) to measure:
*relative concentrations of antibodies/antigens
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*Relative concentrations of antibodies/antigens
*relative purity of an antigen preparation
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*Relative purity of an antigen preparation
  
 
There are two types of immunodiffusion reaction, both being carried out on a semisolid medium:
 
There are two types of immunodiffusion reaction, both being carried out on a semisolid medium:
*'''radial immunodiffusion''': also known as the Mancini method. The medium is prepared with a suitable dilution of antiserum and the antigen sample placed on top and allowed to diffuse. As diffusion takes place,  large insoluble complexes form creating a ring of precipitation (precipitin). By comparing the area of the ring with a standard curve, the concentration of the antigen sample can be determined.  
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*'''Radial immunodiffusion''': also known as the Mancini method. The medium is prepared with a suitable dilution of antiserum and the antigen sample placed on top and allowed to diffuse. As diffusion takes place,  large insoluble complexes form creating a ring of precipitation (precipitin). By comparing the area of the ring with a standard curve, the concentration of the antigen sample can be determined.  
*'''double immunodiffusion''': also known as the Ouchterlony method. Antigen and antibody preparations are placed on the medium and both allowed to diffuse radially from the wells towards each other. This establishes a concentration gradient, forming an area of equivalence and line of precipitin.
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*'''Double immunodiffusion''': also known as the Ouchterlony method. Antigen and antibody preparations are placed on the medium and both allowed to diffuse radially from the wells towards each other. This establishes a concentration gradient, forming an area of equivalence and line of precipitin.
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{{review}}
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{{Jim Bee 2007}}
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[[Category:Immunological Testing]]

Latest revision as of 16:51, 17 March 2012

Introduction

Immunodiffusion reactions are diagnostic tests that use the diffusion of particles through a medium (such as agar) to measure:

  • Relative concentrations of antibodies/antigens
  • Relative purity of an antigen preparation

There are two types of immunodiffusion reaction, both being carried out on a semisolid medium:

  • Radial immunodiffusion: also known as the Mancini method. The medium is prepared with a suitable dilution of antiserum and the antigen sample placed on top and allowed to diffuse. As diffusion takes place, large insoluble complexes form creating a ring of precipitation (precipitin). By comparing the area of the ring with a standard curve, the concentration of the antigen sample can be determined.
  • Double immunodiffusion: also known as the Ouchterlony method. Antigen and antibody preparations are placed on the medium and both allowed to diffuse radially from the wells towards each other. This establishes a concentration gradient, forming an area of equivalence and line of precipitin.





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