Difference between revisions of "Immunological Testing Flashcards"
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==<font color="purple">ELISA testing</font>== | ==<font color="purple">ELISA testing</font>== | ||
| − | + | <FlashCard questions="3"> | |
| − | + | |q1=What does the acronym ELISA stand for? | |
| − | + | |a1=Enzyme Linked ImmunoSorbent Assay | |
| − | + | |l1=ELISA testing | |
| − | | | + | |q2=What type of ELISA requires all the reagents to be added at once? |
| − | | | + | |a2=Homogenous |
| − | ||<font color=" | + | |l2=ELISA testing |
| − | * | + | |q3=What are the five basic steps of an ELISA test? |
| − | || | + | |a3= |
| − | |- | + | *Adsorption of antigen/antibody onto solid phase |
| − | | | + | *Addition of chosen sample and reagents |
| − | ||<font color=" | + | *Incubation and washing |
| − | + | *Addition of enzyme-labelled antigen/antibody | |
| − | || | + | *Addition of specific substrate |
| + | |l3=ELISA testing | ||
| + | </FlashCard> | ||
| + | |||
| + | ==<font color="purple">Immunofluorescence</font>== | ||
| + | <FlashCard questions="4"> | ||
| + | |q1=The adsorption of light by a molecule is known as what? | ||
| + | |a1=Excitation | ||
| + | |l1=Immunofluorescence | ||
| + | |q2=What are the fluorescent dyes commonly known as? | ||
| + | |a2=Fluorochromes | ||
| + | |l2=Immunofluorescence | ||
| + | |q3=What are the advantages of indirect staining? | ||
| + | |a3= | ||
| + | *Brighter fluorescence | ||
| + | *One preparation can be used to test many serum samples | ||
| + | *Relative expressions of different antigens in the same cell can be tested | ||
| + | *Loss of antibody during conjugation is limited | ||
| + | |l3=Immunofluorescence | ||
| + | |q4=What technique is used to provide a quantitative measure of immunofluorescence? | ||
| + | |a4=Flow cytometry | ||
| + | |l4=Immunofluorescence | ||
| + | </FlashCard> | ||
| + | |||
| + | ==<font color="purple">Radioimmunoassay</font>== | ||
| + | <FlashCard questions="4"> | ||
| + | |q1=What are radioimmunoassays commonly used to detect? | ||
| + | |a2= | ||
| + | *Hormone levels in blood and tissue fluids | ||
| + | *Serum proteins | ||
| + | *Drugs | ||
| + | *Vitamins | ||
| + | |l1=Radioimmunoassay | ||
| + | |q2=What is the most common isotope used for radioimmunoassay? | ||
| + | |a2=Iodine-125 | ||
| + | |l2=Radioimmunoassay | ||
| + | |q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? | ||
| + | |a3=Ensures the number of epitopes present exceeds number of antibody-binding sites | ||
| + | |l3=Radioimmunoassay | ||
| + | |q4=What are the disadvantages of the radioimmunoassay? | ||
| + | |a4= | ||
| + | *Substances used are radioactive | ||
| + | *Gamma radiation requires special counting equipment | ||
| + | *Iodine naturally accumulates in the thyroid gland | ||
| + | |l4=Radioimmunoassay | ||
| + | </FlashCard> | ||
| + | |||
| + | ==<font color="purple">Agglutination</font>== | ||
| + | <FlashCard questions="5"> | ||
| + | |q1=What are the antibodies that produce agglutination known as? | ||
| + | |a1=Agglutinins | ||
| + | |l1=Agglutination | ||
| + | |q2=What constitutes the end of the agglutination test? | ||
| + | |a2=The last tube showing distinct agglutination | ||
| + | |l2=Agglutination | ||
| + | |q3=What does the titre represent? | ||
| + | |a3=The number of antibodies per unit volume of serum | ||
| + | |l3=Agglutination | ||
| + | |q4=What is the prozone effect? | ||
| + | |a4=An inhibition of agglutination caused by an excess of antibodies | ||
| + | |l4=Agglutination | ||
| + | |q5=What agglutination test is often used to test for rhesus disease? | ||
| + | |a5=Coombs test | ||
| + | |l5=Agglutination | ||
| + | </FlashCard> | ||
| + | |||
| + | |||
| + | <br><br> | ||
| + | {{Jim Bee 2007}} | ||
| + | [[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]] | ||
Latest revision as of 17:11, 17 March 2012
ELISA testing
| Question | Answer | Article | |
| What does the acronym ELISA stand for? | Enzyme Linked ImmunoSorbent Assay
|
Link to Article | |
| What type of ELISA requires all the reagents to be added at once? | Homogenous
|
Link to Article | |
| What are the five basic steps of an ELISA test? |
|
Link to Article | |
Immunofluorescence
| Question | Answer | Article | |
| The adsorption of light by a molecule is known as what? | Excitation
|
Link to Article | |
| What are the fluorescent dyes commonly known as? | Fluorochromes
|
Link to Article | |
| What are the advantages of indirect staining? |
|
Link to Article | |
| What technique is used to provide a quantitative measure of immunofluorescence? | Flow cytometry
|
Link to Article | |
Radioimmunoassay
| Question | Answer | Article | |
| What are radioimmunoassays commonly used to detect? | Link to Article | ||
| What is the most common isotope used for radioimmunoassay? | Iodine-125
|
Link to Article | |
| Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? | Ensures the number of epitopes present exceeds number of antibody-binding sites
|
Link to Article | |
| What are the disadvantages of the radioimmunoassay? |
|
Link to Article | |
Agglutination
| Question | Answer | Article | |
| What are the antibodies that produce agglutination known as? | Agglutinins
|
Link to Article | |
| What constitutes the end of the agglutination test? | The last tube showing distinct agglutination
|
Link to Article | |
| What does the titre represent? | The number of antibodies per unit volume of serum
|
Link to Article | |
| What is the prozone effect? | An inhibition of agglutination caused by an excess of antibodies
|
Link to Article | |
| What agglutination test is often used to test for rhesus disease? | Coombs test
|
Link to Article | |
|
Originally funded by the RVC Jim Bee Award 2007 |