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| | <categorytree mode=pages>Chlamydophila species</categorytree> | | <categorytree mode=pages>Chlamydophila species</categorytree> |
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| − | ===Overview===
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| − | *Host adapted and non-host adapted species varying in virulence for different hosts
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| − | *Species cause specific diseases in particular hosts
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| − | *Respiratory, enteric, pleural and reproductive diseases in animals and humans
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| − | *Conjunctivitis, arthritis, abortion, urethritis, enteritis, pneumonia, encephalomyelitis
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| − | *Manifestation varies from subclinical to severe systemic infections
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| − | *Intestinal infections often subclinical and persistent
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| − | *Human infections usualy acquired from infected birds, causing psittacosis or ornithosis, causing respiratory infections
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| − | ===Characteristics===
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| − | *Obligate intracellular bacteria
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| − | *Gram negative bacteria with outer membrane, LPS, ribosomes, DNA and RNA
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| − | *Peptidoglycan cell wall resistant to lysozyme
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| − | *Only grow in presence of living eukaryotic cells
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| − | *Unable to synthesis ATP therefore require intermediates from host cells
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| − | *Not stained by Gram stain
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| − | *Two morphological forms
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| − | **Elementary body, the infective extracellular form, which is small, metabolically inert and osmotically stable; surrounded by cytoplasmic membrane, outer membrane with LPS, but no peptidoglycan
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| − | **Retiuculate body: larger, metabolically active, osmotically fragile
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| − | *Elementary body survives in the environment for several days
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| − | ===Pathogenesis and pathogenicity===
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| − | *Elementary body enters host epithelial cells by receptor-mediated endocytosis
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| − | *Transformation into larger reticulate body within endosome; known as an inclusion
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| − | *Reticulate body divides by binary fission to form many new chlamydia cells
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| − | *Reticulate bodies mature and condense to form elementary bodies
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| − | *Elementary bodies released from dying host cells after about 72 hours to infect other cells
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| − | *Persistent infections can occur if replication delayed by environmental conditions such as presence of interferon gamma
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| − | *Many infections subclinical due to intracellular existence of ''chlamydia'' preventing inflammatory reactions
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| − | *Chronic infections may fail to induce an immune response, or may repeatedly stimulate the immune system, causing a delayed hypersensitivity reaction and tissue damage
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| − | *Prolonged faecal shedding of organisms
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| − | *Clinical infections occur in non-natural host species
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| − | *CLinical signs depend on route of infection and degree of exposure
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| − | ===Diagnosis===
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| − | *Direct microscopy of smears and tissues e.g. organs from aborted foetuses, liver/spleen from avian cases
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| − | *Kosters (modified Ziehl-Neelsen) stain of placental smears shows small red rods
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| − | *Blue inclusions in cytoplasm of Giemsa-stained cells
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| − | *Methylene blue stain with darkfield microscopy
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| − | *Fluorescent antibody stain
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| − | *Antigen detection kits for diagnosis from swabs
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| − | *ELISA to detect ''Chlamydophila'' LPS
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| − | *Isolation in embryonated eggs and McCoy cells as well as animal tissues
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| − | *PCR to detect chlamydial DNA
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| − | *Serological tests: complement fixation, ELISA, indirect immunofluorescence
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| − | *May cause [[Joints Inflammatory - Pathology#In Sheep|arthritis in sheep]] and [[Joints Inflammatory - Pathology#In Cattle|arthritis in cattle]]
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| − | '''sheep'''
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| − | *[[:Category:Chlamydophila species|'''''Chlamydia sp.''''']]
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| − | **Sporadic or outbreaks of lamb polyarthritis
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| − | **High morbidity, low mortality
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| − | **Commonly together with conjunctivitis
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| − | **Most recover but may remain lame
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| − | '''cattle'''
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| − | **[[:Category:Chlamydophila species|'''''Chlamydia sp.''''']]
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| − | ***Severe disease in young calves
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| − | ***High mortality
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| − | ***Can be seen in smears of synovial fluid from swollen joints
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| − | ***Oedematous and hyperaemic surrounding tissue
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| − | ***Possibly due to intrauterine infection
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| | [[Category:Bacterial Organisms]] | | [[Category:Bacterial Organisms]] |
| | [[Category:Gram_negative_bacteria]] | | [[Category:Gram_negative_bacteria]] |
| − | [[Category:To_Do_-_Bacteria]]
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