Difference between revisions of "Immunological Testing Flashcards"
Jump to navigation
Jump to search
| Line 1: | Line 1: | ||
==<font color="purple">ELISA testing</font>== | ==<font color="purple">ELISA testing</font>== | ||
| − | + | <FlashCard questions="3"> | |
| − | + | |q1=What does the acronym ELISA stand for? | |
| − | + | |a1=Enzyme Linked ImmunoSorbent Assay | |
| − | + | |l1=ELISA testing | |
| − | + | |q2=What type of ELISA requires all the reagents to be added at once? | |
| − | + | |a2=Homogenous | |
| − | | | + | |l2=ELISA testing |
| − | + | |q3=What are the five basic steps of an ELISA test? | |
| − | | | + | |a3= |
| − | + | *Adsorption of antigen/antibody onto solid phase | |
| − | + | *Addition of chosen sample and reagents | |
| − | | | + | *Incubation and washing |
| − | + | *Addition of enzyme-labelled antigen/antibody | |
| − | | | + | *Addition of specific substrate |
| − | + | |l3=ELISA testing | |
| − | + | </FlashCard> | |
| − | | | ||
| − | * | ||
| − | * | ||
| − | * | ||
| − | * | ||
| − | * | ||
| − | | | ||
| − | |||
==<font color="purple">Immunofluorescence</font>== | ==<font color="purple">Immunofluorescence</font>== | ||
{| border="3" cellpadding="8" | {| border="3" cellpadding="8" | ||
| Line 118: | Line 110: | ||
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] | ||
| + | |||
| + | <br><br> | ||
| + | {{Jim Bee 2007}} | ||
[[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]] | [[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]] | ||
Revision as of 17:00, 17 March 2012
ELISA testing
| Question | Answer | Article | |
| What does the acronym ELISA stand for? | Enzyme Linked ImmunoSorbent Assay
|
Link to Article | |
| What type of ELISA requires all the reagents to be added at once? | Homogenous
|
Link to Article | |
| What are the five basic steps of an ELISA test? |
|
Link to Article | |
Immunofluorescence
| Question | Answer | Article |
|---|---|---|
| The adsorption of light by a molecule is known as what? |
|
Link to Answer Article |
| What are the fluorescent dyes commonly known as? |
|
Link to Answer Article |
| What are the advantages of indirect staining? |
|
Link to Answer Article |
| What technique is used to provide a quantitative measure of immunofluorescence? |
|
Link to Answer Article |
Radioimmunoassay
| Question | Answer | Article |
|---|---|---|
| What are radioimmunoassays commonly used to detect? |
|
Link to Answer Article |
| What is the most common isotope used for radioimmunoassay? |
|
Link to Answer Article |
| Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? |
|
Link to Answer Article |
| What are the disadvantages of the radioimmunoassay? |
|
Link to Answer Article |
Agglutination
| Question | Answer | Article | ||
|---|---|---|---|---|
| What are the antibodies that produce agglutination known as? |
|
Link to Answer Article | ||
| What constitutes the end of the agglutination test? |
|
Link to Answer Article | ||
| What does the titre represent? |
|
Link to Answer Article | ||
| What is the prozone effect? |
|
Link to Answer Article | ||
| What agglutination test is often used to test for rhesus disease? |
|
Link to Answer Article
|
