Difference between revisions of "Burkholderia pseudomallei"

From WikiVet English
Jump to navigation Jump to search
Line 1: Line 1:
 
{{unfinished}}
 
{{unfinished}}
  
''Burkholderia pseudomallei'' is a species of the ''[[Burkholderia species - Overview|Burkholderia]]'' genus. ''B.pseudomallei'' causes [[Melioidosis]], which is endemic in Asia and Australia. It primarily infects rodents.
+
[[File:Burkholderia pseudo.jpg|right|thumb|250px|<small><center> Burkholderia pseudomallei colonies on a Blood agar plate.( Marco Tolo 2007, Wikimedia commons)</center></small>]]
*Pathogenesis:
+
 
**Infection via ingestion, inhalation or skin wounds from environment
+
==Overview==
**Exotoxin, dermonecrotic protease and lecithinase implicated in pathogenicity
+
''Burkholderia pseudomallei'' is a species of the ''[[Burkholderia species - Overview|Burkholderia]]'' genus. ''B.pseudomallei'' causes [[Melioidosis]], an opportunistic infection which is endemic in Asia and Australia. It primarily infects rodents.
**Strain virulence and host immunosuppression important
+
 
*Clinical infections:
+
==Pathogenesis==
**Opportunistic infection with stress and immunosuppression predisposing to disease
+
Infection occurs via ingestion, inhalation or via skin wounds from the environment. The bacteria contains an exotoxin, dermonecrotic protease and lecithinase which is implicated in pathogenicity. Strain virulence and host immunosuppression is important. ''Burkholderia pseudomallei'' is also motile, by polar flagellae.
**Abscesses develop in many organs including lungs, spleen, liver, joints, CNS, upper respiratory tract
+
 
**Chronic, debilitating, progressive disease with long incubation period
+
==Diagnosis==
**Many animal species susceptible
+
Diagnosis of the ''B.pseudomallei'' bacteria can be made via specimens, including;  pus from abscesses, affected tissues and blood.
**Referred to as pseudoglanders in horses
+
The fluorescent antibody technique can be done on tissue smears. ''B.pseudomallei'' grows on blood agar and MacConkey agar plates, incubated aerobically. The colonies have a musty smell and you see lactose fermentation on MacConkey. Slide agglutination, ELISA, complement fixation and indirect haemagglutination tests for serum antibodies are also all diagnostic of ''B.pseudomallei.''
*Diagnosis:
+
 
**Specimens: pus from abscesses, affected tissues, blood
+
[[Category:Pseudomonas_and_Burkholderia_species]]
**Fluorescent antibody technique on tissue smears
+
 
**Blood agar and MacConkey agar plates, incubated aerobically
+
[[Category:To_Do_-_AimeeHicks]]
**Colonies have musty smell
 
**Lactose fermentation on MacConkey
 
**Slide agglutination
 
**ELISA, complement fixation and indirect haemagglutination tests for serum antibodies
 
*Treatment/control: slaughter of infected animals where exotic[[Category:Pseudomonas_and_Burkholderia_species]][[Category:Rodents]]
 
[[Category:To_Do_-_Bacteria]]
 

Revision as of 09:56, 26 July 2010



Burkholderia pseudomallei colonies on a Blood agar plate.( Marco Tolo 2007, Wikimedia commons)

Overview

Burkholderia pseudomallei is a species of the Burkholderia genus. B.pseudomallei causes Melioidosis, an opportunistic infection which is endemic in Asia and Australia. It primarily infects rodents.

Pathogenesis

Infection occurs via ingestion, inhalation or via skin wounds from the environment. The bacteria contains an exotoxin, dermonecrotic protease and lecithinase which is implicated in pathogenicity. Strain virulence and host immunosuppression is important. Burkholderia pseudomallei is also motile, by polar flagellae.

Diagnosis

Diagnosis of the B.pseudomallei bacteria can be made via specimens, including; pus from abscesses, affected tissues and blood. The fluorescent antibody technique can be done on tissue smears. B.pseudomallei grows on blood agar and MacConkey agar plates, incubated aerobically. The colonies have a musty smell and you see lactose fermentation on MacConkey. Slide agglutination, ELISA, complement fixation and indirect haemagglutination tests for serum antibodies are also all diagnostic of B.pseudomallei.