Difference between revisions of "Immunological Testing Flashcards"

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==<font color="purple">ELISA testing</font>==
 
==<font color="purple">ELISA testing</font>==
{| border="3" cellpadding="8"
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<FlashCard questions="3">
!width="400"|'''Question'''
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|q1=What does the acronym ELISA stand for?
!width="400"|'''Answer'''
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|a1=Enzyme Linked ImmunoSorbent Assay
!width="150"|'''Article'''
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|l1=ELISA testing
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|q2=What type of ELISA requires all the reagents to be added at once?
|<big>'''What does the acronym ELISA stand for?'''
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|a2=Homogenous
||<font color="white"> <big>
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|l2=ELISA testing
*'''''Enzyme Linked ImmunoSorbent Assay'''''
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|q3=What are the five basic steps of an ELISA test?
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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|a3=
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*Adsorption of antigen/antibody onto solid phase
|<big>'''What type of ELISA requires all the reagents to be added at once?'''
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*Addition of chosen sample and reagents
||<font color="white"> <big>
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*Incubation and washing
*'''''Homogenous'''''
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*Addition of enzyme-labelled antigen/antibody
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
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*Addition of specific substrate
|-
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|l3=ELISA testing
|<big>'''What are the five basic steps of an ELISA test?'''
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</FlashCard>
||<font color="white"> <big>
 
*'''''Adsorption of antigen/antibody onto solid phase'''''
 
*'''''Addition of chosen sample and reagents'''''
 
*'''''Incubation and washing'''''
 
*'''''Addition of enzyme-labelled antigen/antibody'''''
 
*'''''Addition of specific substrate'''''
 
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
 
  
|}
 
 
==<font color="purple">Immunofluorescence</font>==
 
==<font color="purple">Immunofluorescence</font>==
 
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||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
  
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{{Jim Bee 2007}}
 
[[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]]
 
[[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]]

Revision as of 17:00, 17 March 2012

ELISA testing

Question Answer Article
What does the acronym ELISA stand for? Link to Article
What type of ELISA requires all the reagents to be added at once? Link to Article
What are the five basic steps of an ELISA test? Link to Article


Immunofluorescence

Question Answer Article
The adsorption of light by a molecule is known as what?
  • Excitation
Link to Answer Article
What are the fluorescent dyes commonly known as?
  • Fluorochromes
Link to Answer Article
What are the advantages of indirect staining?
  • Brighter fluorescence
  • One preparation can be used to test many serum samples
  • Relative expressions of different antigens in the same cell can be tested
  • Loss of antibody during conjugation is limited
Link to Answer Article
What technique is used to provide a quantitative measure of immunofluorescence?
  • Flow cytometry
Link to Answer Article

Radioimmunoassay

Question Answer Article
What are radioimmunoassays commonly used to detect?
  • Hormone levels in blood and tissue fluids
  • Serum proteins
  • Drugs
  • Vitamins
Link to Answer Article
What is the most common isotope used for radioimmunoassay?
  • Iodine-125
Link to Answer Article
Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
  • Ensures the number of epitopes present exceeds number of antibody-binding sites
Link to Answer Article
What are the disadvantages of the radioimmunoassay?
  • Substances used are radioactive
  • Gamma radiation requires special counting equipment
  • Iodine naturally accumulates in the thyroid gland
Link to Answer Article

Agglutination

Question Answer Article
What are the antibodies that produce agglutination known as?
  • Agglutinins
Link to Answer Article
What constitutes the end of the agglutination test?
  • The last tube showing distinct agglutination
Link to Answer Article
What does the titre represent?
  • The number of antibodies per unit volume of serum
Link to Answer Article
What is the prozone effect?
  • An inhibition of agglutination caused by an excess of antibodies
Link to Answer Article
What agglutination test is often used to test for rhesus disease?
  • Coombs test
Link to Answer Article




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