Difference between revisions of "Immunological Testing Flashcards"
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==<font color="purple">Immunofluorescence</font>== | ==<font color="purple">Immunofluorescence</font>== | ||
| − | + | <FlashCard questions="4"> | |
| − | + | |q1=The adsorption of light by a molecule is known as what? | |
| − | + | |a1=Excitation | |
| − | + | |l1=Immunofluorescence | |
| − | + | |q2=What are the fluorescent dyes commonly known as? | |
| − | + | |a2=Fluorochromes | |
| − | | | + | |l2=Immunofluorescence |
| − | + | |q3=What are the advantages of indirect staining? | |
| − | | | + | |a3= |
| − | + | *Brighter fluorescence | |
| − | + | *One preparation can be used to test many serum samples | |
| − | | | + | *Relative expressions of different antigens in the same cell can be tested |
| − | + | *Loss of antibody during conjugation is limited | |
| − | | | + | |l3=Immunofluorescence |
| − | + | |q4=What technique is used to provide a quantitative measure of immunofluorescence? | |
| − | + | |a4=Flow cytometry | |
| − | | | + | |l4=Immunofluorescence |
| − | * | + | </FlashCard> |
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==<font color="purple">Radioimmunoassay</font>== | ==<font color="purple">Radioimmunoassay</font>== | ||
Revision as of 17:04, 17 March 2012
ELISA testing
| Question | Answer | Article | |
| What does the acronym ELISA stand for? | Enzyme Linked ImmunoSorbent Assay
|
Link to Article | |
| What type of ELISA requires all the reagents to be added at once? | Homogenous
|
Link to Article | |
| What are the five basic steps of an ELISA test? |
|
Link to Article | |
Immunofluorescence
| Question | Answer | Article | |
| The adsorption of light by a molecule is known as what? | Excitation
|
Link to Article | |
| What are the fluorescent dyes commonly known as? | Fluorochromes
|
Link to Article | |
| What are the advantages of indirect staining? |
|
Link to Article | |
| What technique is used to provide a quantitative measure of immunofluorescence? | Flow cytometry
|
Link to Article | |
Radioimmunoassay
| Question | Answer | Article |
|---|---|---|
| What are radioimmunoassays commonly used to detect? |
|
Link to Answer Article |
| What is the most common isotope used for radioimmunoassay? |
|
Link to Answer Article |
| Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? |
|
Link to Answer Article |
| What are the disadvantages of the radioimmunoassay? |
|
Link to Answer Article |
Agglutination
| Question | Answer | Article | ||
|---|---|---|---|---|
| What are the antibodies that produce agglutination known as? |
|
Link to Answer Article | ||
| What constitutes the end of the agglutination test? |
|
Link to Answer Article | ||
| What does the titre represent? |
|
Link to Answer Article | ||
| What is the prozone effect? |
|
Link to Answer Article | ||
| What agglutination test is often used to test for rhesus disease? |
|
Link to Answer Article
|
