Difference between revisions of "Immunological Testing Flashcards"
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| Line 73: | Line 73: | ||
|q4=What is the prozone effect? | |q4=What is the prozone effect? | ||
|a4=An inhibition of agglutination caused by an excess of antibodies | |a4=An inhibition of agglutination caused by an excess of antibodies | ||
| − | |l4=Agglutination | + | |l4=Agglutination |
|q5=What agglutination test is often used to test for rhesus disease? | |q5=What agglutination test is often used to test for rhesus disease? | ||
|a5=Coombs test | |a5=Coombs test | ||
Latest revision as of 17:11, 17 March 2012
ELISA testing
| Question | Answer | Article | |
| What does the acronym ELISA stand for? | Enzyme Linked ImmunoSorbent Assay
|
Link to Article | |
| What type of ELISA requires all the reagents to be added at once? | Homogenous
|
Link to Article | |
| What are the five basic steps of an ELISA test? |
|
Link to Article | |
Immunofluorescence
| Question | Answer | Article | |
| The adsorption of light by a molecule is known as what? | Excitation
|
Link to Article | |
| What are the fluorescent dyes commonly known as? | Fluorochromes
|
Link to Article | |
| What are the advantages of indirect staining? |
|
Link to Article | |
| What technique is used to provide a quantitative measure of immunofluorescence? | Flow cytometry
|
Link to Article | |
Radioimmunoassay
| Question | Answer | Article | |
| What are radioimmunoassays commonly used to detect? | Link to Article | ||
| What is the most common isotope used for radioimmunoassay? | Iodine-125
|
Link to Article | |
| Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? | Ensures the number of epitopes present exceeds number of antibody-binding sites
|
Link to Article | |
| What are the disadvantages of the radioimmunoassay? |
|
Link to Article | |
Agglutination
| Question | Answer | Article | |
| What are the antibodies that produce agglutination known as? | Agglutinins
|
Link to Article | |
| What constitutes the end of the agglutination test? | The last tube showing distinct agglutination
|
Link to Article | |
| What does the titre represent? | The number of antibodies per unit volume of serum
|
Link to Article | |
| What is the prozone effect? | An inhibition of agglutination caused by an excess of antibodies
|
Link to Article | |
| What agglutination test is often used to test for rhesus disease? | Coombs test
|
Link to Article | |
|
Originally funded by the RVC Jim Bee Award 2007 |