Difference between revisions of "Chlamydia and Chlamydophila species"
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*Only grow in presence of living eukaryotic cells | *Only grow in presence of living eukaryotic cells | ||
*Unable to synthesis ATP therefore require intermediates from host cells | *Unable to synthesis ATP therefore require intermediates from host cells | ||
− | * | + | *Grow in embyonated eggs and McCoy cells as well as animal tissues |
+ | *Not stained by Gram stain | ||
+ | *Kosters (modified Ziehl-Neelson, small red rods) or fluorescent antibody stain required for detection | ||
+ | *Blue inclusions in cytoplasm of Giemsa-stained cels | ||
+ | *Antigen detection kits for diagnosis from swabs | ||
Line 30: | Line 34: | ||
*Reticulate body has un-crosslinked peptidoglycan, which is cross-linked to form elementary bodies | *Reticulate body has un-crosslinked peptidoglycan, which is cross-linked to form elementary bodies | ||
*Elementary bodies released from dying host cells to infect other cells | *Elementary bodies released from dying host cells to infect other cells | ||
+ | *Persistent infections | ||
+ | *Many infections subclinical due to intracellular existence of ''chlamydia'' preventing inflammatory reactions | ||
+ | *Clinical infections occur in non-natural host species | ||
+ | |||
+ | |||
+ | ===''Chlamydophila psittaci''=== | ||
+ | |||
+ | *Causes notifiable psittacosis in birds | ||
+ | |||
+ | |||
+ | ===''Chlamydophila abortus''=== | ||
+ | |||
+ | *Ovine enzootic abortion | ||
+ | *Contagious ovine abortion in late pregnancy | ||
+ | *Infection via ingestion or inhalation causes a bacteraemia | ||
+ | *Bacteria localise in placenta and cause placentitis, leading to abortion | ||
+ | *Abortion rates may reach 30% | ||
+ | *Fertility not impaired | ||
+ | *Survival of elementary bodies in faeces and wild birds are a source of infection from one lambing season to the next | ||
+ | *Ewes may be carriers for several years | ||
+ | *Venereal transmission from infected rams | ||
+ | *Some immunity develops after infection, protecting ewes from subsequent disease | ||
+ | *Vaccines prevent infection but will not clear infection from persistently-infected animals | ||
+ | *Vaccination of ewe lambs | ||
+ | |||
+ | |||
+ | ===''Chlamydophila felis''=== | ||
+ | |||
+ | *Host adapted species in cats | ||
+ | *Feline interstitial pneumonia and conjunctivitis | ||
+ | *Diagnosis by antigen detection in ocular/nasal secretions - ELISA, PCR, Kosters, fluorescent antibody test | ||
+ | *Vaccine | ||
− | |||
Revision as of 09:36, 18 May 2008
Chlamydophila psittaci involved in feline rhinotracheitis together with herpes virus 1 C.psittaci more frequently infects the conjunctival epithelium -> chronic conjunctivitis, see feline chlamydiosis
- May cause arthritis in sheep and arthritis in cattle
Overview
Characteristics
- Obligate intracellular bacteria
- Gram negative bacteria with outer membrane, LPS, ribosomes, DNA and RNA
- Peptidoglycan cell wall resistant to lysozyme
- Only grow in presence of living eukaryotic cells
- Unable to synthesis ATP therefore require intermediates from host cells
- Grow in embyonated eggs and McCoy cells as well as animal tissues
- Not stained by Gram stain
- Kosters (modified Ziehl-Neelson, small red rods) or fluorescent antibody stain required for detection
- Blue inclusions in cytoplasm of Giemsa-stained cels
- Antigen detection kits for diagnosis from swabs
Pathogenesis and pathogenicity
- Elementary body, the infective form, phagocytosed and internalised into host epithelial cells
- Transformation into larger reticulate body
- Reticulate body divides by binary fission to form many new chlamydia cells
- Reticulate body has un-crosslinked peptidoglycan, which is cross-linked to form elementary bodies
- Elementary bodies released from dying host cells to infect other cells
- Persistent infections
- Many infections subclinical due to intracellular existence of chlamydia preventing inflammatory reactions
- Clinical infections occur in non-natural host species
Chlamydophila psittaci
- Causes notifiable psittacosis in birds
Chlamydophila abortus
- Ovine enzootic abortion
- Contagious ovine abortion in late pregnancy
- Infection via ingestion or inhalation causes a bacteraemia
- Bacteria localise in placenta and cause placentitis, leading to abortion
- Abortion rates may reach 30%
- Fertility not impaired
- Survival of elementary bodies in faeces and wild birds are a source of infection from one lambing season to the next
- Ewes may be carriers for several years
- Venereal transmission from infected rams
- Some immunity develops after infection, protecting ewes from subsequent disease
- Vaccines prevent infection but will not clear infection from persistently-infected animals
- Vaccination of ewe lambs
Chlamydophila felis
- Host adapted species in cats
- Feline interstitial pneumonia and conjunctivitis
- Diagnosis by antigen detection in ocular/nasal secretions - ELISA, PCR, Kosters, fluorescent antibody test
- Vaccine