Difference between revisions of "Leishmania"
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==''Leishmania''== | ==''Leishmania''== | ||
[[Image:Leishmania donovani.jpg|thumb|right|150px|''Leishmania donovani'' in bone marrow cell - Dr. L.L. Moore, Jr.]] | [[Image:Leishmania donovani.jpg|thumb|right|150px|''Leishmania donovani'' in bone marrow cell - Dr. L.L. Moore, Jr.]] | ||
+ | [[Image:Leishmania tropica.jpg|right|thumb|150px|''L. tropica'' - Yutaka Tsutsumi, M.D., Professor, Department of Pathology, Fujita Health University School of Medicine]] | ||
*''Leishmania'' spp. are intracellular parasites of [[Macrophage|macrophages]] | *''Leishmania'' spp. are intracellular parasites of [[Macrophage|macrophages]] | ||
Revision as of 17:27, 23 November 2008
This article is still under construction. |
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Trypanosoma
- Protozoal parasites found in the blood and tissues of vertebrates
- Worldwide distribution
- Causes sleeping sickness in humans
- Particularly affect sub-Saharan Africa
- Affect cattle production
- Cause Nagana (depression)
- Divided into two groups depending on the mode of development of the insect vector
- Salivarian
- Multiply in the foregut
- Transmitted via innoculation via feeding
- Stercorarian
- Multiply in the hindgut
- Transmitted via contamination of wounds with insect faeces
- Salivarian
Life Cycle
- Undergo morphological transformations in intermediate host before becoming infective for the next host
- Blood-sucking flies ingest trypanosomes whilst taking a blood meal from an infected animal
- Trypanosomes multiply first in the gut of the fly
- Salivarian trypanosomes are transmitted by Tsetse flies
- Trypanosomes pass foward to the salivary glands where they transform into the infective stage
- Innoculated with saliva when Tsetse fly next feeds on a host
- Stercorarian trypanosomes are transmitted by triatomid bugs, tabanids and keds
- Trypanosomes pass back to the rectum
- Next host is infected when skin wounds are contaminated with infected insect faeces
Pathogenesis
- Salivarian
- Causes wasting disease in cattle (nagana)
- Sleeping sickness in humans
- Stercorarian
- T. cruzi most important in veterinary medicine
- Occurs in South America
- Infects armadillos, possums and humans
- Causes Chagas Disease
- Transmitted by a triatomid (kissing) bug
- Chronic infections are often fatal causing heart failure
- Non-pathogenic species are transmitted by tabanids and keds
- T. theileria and T. melophagium
- T. cruzi most important in veterinary medicine
- Enlarged lymph nodes and spleen
- Causes lymphoid exhaustion
- Anaemia
- Red blood cells removed from circulation
- Degeneration and inflammation of multiple organs
- E.g. Skeletal muscle, myocardium and CNS
Epidemiology
- Vector distribution
- Tsetse flies found in riverine, savannah and forest habitats
- Up to 20% flies infected
- Flies infected for life
- Parasite virulence
- Some parasitaemic animals survive for long periods of time
- E.g. T. brucei and T. congolense
- Increases the opportunity for infection of flies
- Some trypanosomes kill their host in 1-2 weeks
- E.g. T. vivax
- Decreases the chances of fly infection
- Trypanosomes avoid host immune defences by altering glycoprotein coat (surface antigen) before host antibody response
- Antigenic variation can occur many times over several months causes relapsing parasitaemia
- Some parasitaemic animals survive for long periods of time
- Host response
- Trypanotolerant wild animals remain parasitaemic for prolpnged periods without showing clincial signs of disease
- Cause lasting reservoirs of infection
- Most domestic livestock are susceptible to trypanosomosis
- Some local breeds of sheep, goats and cattle are trypanotolerant
- E.g. Bos indicus
- Trypanotolerant wild animals remain parasitaemic for prolpnged periods without showing clincial signs of disease
Diagnosis
- Demonstrate trypanosomes in blood
- Giemsa stained smears
- Fresh blood films
- Motile trypanosomes
- Haematocrit tube
- Motile trypanosomes at the plasma/buffy coat interface
Control
- Tsetse fly control
- Spraying and trapping
- Prophylactic drug treatment
- Change drug group periodically to decrease the chances of resistance occuring
- May lead to protective immunity but livestock will still be susceptible to heterologous challenges
- Barrier fences and buffer zones
- Separate livestock and wild animals
- Trypanotolerant livestock
Other trypanosomes
- Mechanically transmitted by biting flies
- E.g. Surra affecting horses and camels in North Africa, Asia and South America
- T. equinum in South America
- T. evansi in Asia
- Venerally transmitted
- E.g. Dourine
- Transmitted by T. equiperdum
- Causes genital and abdominal oedema, emaciataion and CNS signs
- Affects horses and donkeys in Africa, Asia, Central and South America
- E.g. Dourine
- Non-pathogenic species occur in the UK
- In sheep caused by T. melophagium
- In cattle caused by T. theileri
Leishmania
- Leishmania spp. are intracellular parasites of macrophages
- Are closely related to Trypanosoma spp.
- Cause diseases in humans, dogs and wild animals
- Present in southern Europe, Africa, Asia and South America
Life Cycle
- Transmitted by blood sucking sand flies
- Phlebotomus spp. in the Old World
- Lutzomyia spp. in the New World
- The amastigote (morphological form) in found in vertebrate macrophages
- Multiplies and migrates to insect proboscis
- Innoculated during feeding
- Can be transmitted percutaneously if sand fly crushed on skin
- Invades macrophages and reverts to amastigote
Pathogenesis
- Infection of vertebrate host
- Produces foci of proliferating Leishmania-infected macrophages in skin (cutaneous) or internal organs (visceral)
- Very long incubation periods
- Months to years
- Many infected dogs are asymptomatic
- Cutaneous form
- Produces areas of ulceration on pinnae of ears
- Visceral form causes chronic wasting condition
- Generalised eczema
- Loss of hair around eyes producing 'spectacle' effect
- Intermittent fever
- Generalised lymphadenopathy
- Generalised eczema
- Involved in skin infections
Epidemiology
- Disease dependent on sand fly vectors
- E.g. Common in dogs around the Mediterranean coast, foci around southern Europe and around Madrid
- Reservoirs of infection
- E.g. Wild animals such as rodents and stray dogs
- Mechanisms of transmission
- Direct contact
- sand fly bite
- Leishmaniasis in British dogs
- Susceptible to infection if exposed whilst abroad in endemic areas as have no immunity
- No sand flies in Britain but dogs have become infected whilst in contact with infected imported animals
Diagnosis
- Demonstrate Leishmania organisms
- In skin scraping or smears
- In lymph node or bone marrow biopsies
Treatment and Control
- Chemotherapy
- Prolonged treatment, expensive, suppresses infection
- Does not cure infection
- Prevent sand flies biting
- Collars, sprays containing insecticide with repellent effect
- Destruction of infected and stray dogs
- Sand flies biting infected dogs may spread the disease to to other dogs, humans and wildlife
- There is a slight possibility of transmission to humans by direct contact