Difference between revisions of "Category:Mycoplasmas"

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Overview

• Pathogens belong to the Mycoplasma and Ureaplasma genera
• Cause many diseases especially respiratory diseases of farm animals including contagious bovine pleuropneumonia
• Can be involved in mastitis and conjunctivitis in cattle
• Implicated in respiratory and urinary tract diseases in dogs and cats
• Non-pathogenic mycoplasmas present in the rumen
• Live on mucous membranes of oronasal cavity, conjunctiva and intestines
• Stress factors and concurrent disease may predispose to tissue invasion
• Usually host-specific
• Limited survival in the environment

Characteristics

• Smallest free-living prokaryotic organism
• Pleomorphic organisms
• Have no peptidoglycan cell wall
• Susceptible to dessication, heat and disinfectants
• Require enriched media containing animal protein, sterol and a source of DNA for growth
• Colonies have a fried egg appearance
• Most are facultative anaerobes

Pathogenesis and pathogenicity

• Mycoplasmas adhere to host cells and produce toxins
• Some adhere to neutrophils and macrophages and prevent phagocytosis
• Mycoplasmas induce proliferation of macrophages and [[monocytes]], and release of cytokines such as TNF and interleukins
• Cause damage to cilia in the respiratory tract leading to pneumonia
• Molecular mimicry allows some mycoplasmas to avoid the host immune response and may initiate immune-mediated disease

Diagnosis

• Samples can be tested for the presence of mycoplasmas by fluorescent antibody techniques, peroxidase techniques and PCR
• Biochemical profiles such as urease production can be used for identification
• Ureaplasmas produce urease, whereas Mycoplasmas do not metabolise urea
• Serolgy is required for specific identification including complement fixation tests, ELISA, agglutination tests and haemagglutination-inhibition tests
• Growth inhibition tests using specific antisera can be used as well as fluorescent antibody tests

Contagious bovine pleuropneumonia

• Contagious bovine pleuropneumonia is caused by M. mycoides subsp. mycoides small colony type
• A severe contagious disease of cattle
• Endemic in Africa, the Middle East and Asia
• Aerosol transmission by close contact with clinically or subclinically affected animals
• Severity depends on strain and host susceptibility
• Slow spread of infection
• 50% morbidity; mortality rate high in severe outbreaks
• Large colony type causes pleuropneumonia, mastitis, septicaemia and polyarthritis

• Clinical signs
  • Acute onset fever, anorexia, depression, lowered milk yield, hyperpnoea, coughing and a mucopurulent nasal discharge
  • Dyspnoea occurs with abducted elbows and extended necks and an expiratory grunt
  • Can be fatal within 1-3 weeks
  • Calves may suffer from arthritis, synovitis and endocarditis

• Gross pathology
  • Marbled appearance to lungs with consolidated grey and red lobules separated by emphysematous areas
  • Serofibrinous pleural fluid
  • Necrotic foci surrounded by fibrous capsules in chronic cases act as source of infection

• Diagnosis
  • Clinical signs and post-mortem appearance
  • PCR on pleural fluid, lung tissue, regional lymph nodes or bronchoalveolar lavage fluid
  • Fluorescent antibody test
  • Serological tests such as serum agglutination, haemagglutination, complement fixation, ELISA

• Treatment and control
  • Slaughter of affected cattle in counries where the disease is exotic
  • Movement restrictions, quaranteen and slaughter of carrier animals in endemic countries
  • Vaccination in endemic regions

Mycoplasma bovis

• Causes severe pneumonia in calves as a primary pathogen
• Can occur secondarily to Pasteurella and Mannheimia and IBR
• Associated with arthritis and mastitis
• Mastitis associated with a severe drop in milk yield and a purulent discharge

Contagious agalactia of sheep and goats

• Severe febrile disease of sheep and goats in parts of Europe, Africa, Asia
• Caused by M. agalactiae
• Mastitis, arthritis and conjunctivitis following parturition
• Causes abortion
• Fatal pneumonia in young animals
• Shed in milk; localised in supramammary lymph nodes
• Inactivated, attenuated vaccines available

Contagious caprine pleuropneumonia

• Caused by M. capricolum subsp. capricolum'; occasionally M. mycoides subsp. capri or M. mycoides subsp. mycoides
• Occurs in Africa and Turkey
• Pneumonia, fibrinous pleurisy, pleural exudate, consolidated and emphysematous lungs
• Aerosol transmission; highly contagious
• Identified by growth inhibition disc tests
• Inactivated vaccines available
• M. mycoides subsp. 'mycoides causes septicaemia, pleuropneumonia, arthritis and mastitis in goats

Enzootic pneumonia of pigs

• Enzootic pneumonia of pigs caused by M. hyopneumoniae
• Occur in unfavourable conditions with poor ventilation, overcrowding and temperature fluctuations
• Stunted growth rates, coughing and occasionally respiratory distress
• Consolidation in apical and cardiac lung lobes
• Immunofluorescence of lung tissue and complement fixation test or ELISA used for identification
• Treatment includes in-feed antibiotics such as tylosin, lincomycin and tiamulin
• Absent in specific-pathogen-free herds

M. hyorhinis and M. hyosynoviae of pigs

• M. hyosynovia and M. hyorhinis produce syndromes similar to Glasser's disease with milder expression, rarely menigitis and arthritis
• M. hyorhinis causes a chronic progressive polyserositis in pigs under 10 weeks old
• A fever, laboured breathing, lameness and swollen joints occur
• Serofibrinous pleurisy, pericarditis and peritonitis occur
• Treatment includes tylosin and lincomycin
• M. hyorhinis causes a polyarthritis in pigs of 10-30 weeks old, leading to transient lameness

Mycoplasmas of poultry

• M. gallisepticum
  • Causes chronic repsiratory disease in chickens and infectious sinusitis in turkeys
  • Transmission via the egg and aerosol
  • Reduced egg production
  • Identification by serum plate agglutination test, haemagglutination inhibition and ELISA
  • Controlled in specific-pathogen-free flocks
  • Modified live vaccines and bacterins available
• M. meleagridis
  • Hatching problems; airsacculitis in young poults; joint and bone deformities in growers; poor growth rates
  • Transmitted mainly via eggs and semen
  • Serum plate agglutination test for identification
  • In-water tylosin for first 10 days of life
  • Eggs can be dipped in tylosin
• M synoviae
  • Causes infectious synovitis in chickens and turkeys
  • Transmitted by aerosol
  • Synovitis, arthritis, respiratory disease
  • Confirmation by isolation or serology
  • In-feed tetracycline
  • Eradication through specific-pathogen-free flocks

Other mycoplasmas

• M. bovis, M. dispar and Ureaplasma can be involved in Enzootic pneumonia of calves
• M. ovipneumoniae is implicated in enzootic pneumonia of lambs
• M. felis may be involved in mild respiratory infection
• Mycoplasmas may cause lymphocytic chronic inflammation (peribronchiolar and perialveolar cuffing)
• Cause peritonitis in sheep, peritonitis in goats and peritonitis in pigs