If a molecule has the property of fluorescence, it can absorb light of one wavelength (excititation) and emit light of another (emission). Antibodies tagged with these dyes (known as '''fluorochromes''') form immune complexes with specific antigens that can be detected when excited by light of a certain wavelength.
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If a molecule has the property of fluorescence, it can absorb light of one wavelength (excititation) and emit light of another (emission). Antibodies tagged with these dyes (known as '''fluorochromes''') form immune complexes with specific antigens which can then be indirectly visualised when excited by light of the appropriate wavelength.