Difference between revisions of "In Vitro Fertilization - Anatomy & Physiology"

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m (Text replace - "-_Anatomy_%26_Physiology" to "- Anatomy & Physiology")
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== Oocyte Collection==
 
== Oocyte Collection==
  
−
* Donor is superovulated to provide large numbers of mature [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicles]].
+
* Donor is superovulated to provide large numbers of mature [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]].
−
* Pre-ovulatory [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicles]] are collected by a surgical procedure involving aspiration of the follicular content.
+
* Pre-ovulatory [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]] are collected by a surgical procedure involving aspiration of the follicular content.
−
* [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|Oocytes]] captured in vitro.
+
* [[The_Ovary_-_Oocytes_- Anatomy & Physiology|Oocytes]] captured in vitro.
  
  
−
* Direct [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] aspiration:  
+
* Direct [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]] aspiration:  
−
** A hyperdermic needle is inserted into the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]].
+
** A hyperdermic needle is inserted into the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]].
 
** Follicular fluid is aspirated.
 
** Follicular fluid is aspirated.
−
** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]].
+
** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]].
−
** Repeated 2-3 times to dislodge [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocytes]].
+
** Repeated 2-3 times to dislodge [[The_Ovary_-_Oocytes_- Anatomy & Physiology|oocytes]].
  
  
 
* Transvaginal Aspiration in the Mare:
 
* Transvaginal Aspiration in the Mare:
−
** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_-_Anatomy_%26_Physiology|rectum]].
+
** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_- Anatomy & Physiology|rectum]].
−
** Lubricated ultrasound transducer is inserted into the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vagina]] and held in the fornix vagina.
+
** Lubricated ultrasound transducer is inserted into the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_- Anatomy & Physiology|vagina]] and held in the fornix vagina.
−
** [[Female_Reproductive_Tract_-The_Ovary_-_Anatomy_%26_Physiology|Ovary]] transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] can be visualized.
+
** [[Female_Reproductive_Tract_-The_Ovary_- Anatomy & Physiology|Ovary]] transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]] can be visualized.
−
** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vaginal wall]] into the antral follicle.
+
** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_- Anatomy & Physiology|vaginal wall]] into the antral follicle.
−
** Follicular fluid containing the [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocyte]] is aspirated under constant vacuum.
+
** Follicular fluid containing the [[The_Ovary_-_Oocytes_- Anatomy & Physiology|oocyte]] is aspirated under constant vacuum.
  
  
−
* Aspirated [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocytes]] are placed in a culture vessel with [[Copulation_-Sperm_in_the_Female_Tract_-_Anatomy_%26_Physiology#Capacitation|capacitated]] spermatozoa.
+
* Aspirated [[The_Ovary_-_Oocytes_- Anatomy & Physiology|oocytes]] are placed in a culture vessel with [[Copulation_-Sperm_in_the_Female_Tract_- Anatomy & Physiology#Capacitation|capacitated]] spermatozoa.
 
** Could also be cultured in a 'nurse animal' in vivo.
 
** Could also be cultured in a 'nurse animal' in vivo.
 
* Embryos are incubated for a further period to the appropriate stage for transfer.
 
* Embryos are incubated for a further period to the appropriate stage for transfer.
 
* Embryos transferred to recipient female.
 
* Embryos transferred to recipient female.
−
* Can be transferred at the correct stage of the recipient's [[Female_Reproductive_Tract_-Oestrous_Cycle_-_Anatomy_%26_Physiology|oestrous cycle]].
+
* Can be transferred at the correct stage of the recipient's [[Female_Reproductive_Tract_-Oestrous_Cycle_- Anatomy & Physiology|oestrous cycle]].
 
* Embryos transferred around the [[Blastocyst Embryonic Development - Anatomy & Physiology|blastocyst]] stage.
 
* Embryos transferred around the [[Blastocyst Embryonic Development - Anatomy & Physiology|blastocyst]] stage.
 
* Success rates are improving.
 
* Success rates are improving.
 
* Embryos can be frozen, eliminating the need for tight synchronisation.  This allows them to be shipped to other locations.
 
* Embryos can be frozen, eliminating the need for tight synchronisation.  This allows them to be shipped to other locations.
−
* Increases incidence of [[Parturition_-_Risks_in_Parturition_-_Anatomy_%26_Physiology#Large_Offspring|large offspring syndrome]].
+
* Increases incidence of [[Parturition_-_Risks_in_Parturition_- Anatomy & Physiology#Large_Offspring|large offspring syndrome]].
  
  
 
[[Category:Reproductive System]]
 
[[Category:Reproductive System]]

Revision as of 15:23, 29 November 2010

()Map REPRODUCTIVE SYSTEM (Map)
REPRODUCTIVE TECHNOLOGIES




Oocyte Collection

  • Donor is superovulated to provide large numbers of mature follicles.
  • Pre-ovulatory follicles are collected by a surgical procedure involving aspiration of the follicular content.
  • Oocytes captured in vitro.


  • Direct follicle aspiration:
    • A hyperdermic needle is inserted into the follicle.
    • Follicular fluid is aspirated.
    • Follicular fluid forcefully returned to the follicle.
    • Repeated 2-3 times to dislodge oocytes.


  • Transvaginal Aspiration in the Mare:
    • Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the rectum.
    • Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.
    • Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized.
    • Hypodermic needle is advanced through the vaginal wall into the antral follicle.
    • Follicular fluid containing the oocyte is aspirated under constant vacuum.


  • Aspirated oocytes are placed in a culture vessel with capacitated spermatozoa.
    • Could also be cultured in a 'nurse animal' in vivo.
  • Embryos are incubated for a further period to the appropriate stage for transfer.
  • Embryos transferred to recipient female.
  • Can be transferred at the correct stage of the recipient's oestrous cycle.
  • Embryos transferred around the blastocyst stage.
  • Success rates are improving.
  • Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations.
  • Increases incidence of large offspring syndrome.
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