Difference between revisions of "Immunological Testing Flashcards"

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==<font color="purple">ELISA testing</font>==
 
==<font color="purple">ELISA testing</font>==
−
<FlashCard questions="3">
+
{| border="3" cellpadding="8"
−
|q1=What does the acronym ELISA stand for?
+
!width="400"|'''Question'''
−
|a1=Enzyme Linked ImmunoSorbent Assay
+
!width="400"|'''Answer'''
−
|l1=ELISA testing
+
!width="150"|'''Article'''
−
|q2=What type of ELISA requires all the reagents to be added at once?
+
|-
−
|a2=Homogenous
+
|<big>'''What does the acronym ELISA stand for?'''
−
|l2=ELISA testing
+
||<font color="white"> <big>
−
|q3=What are the five basic steps of an ELISA test?
+
*'''''Enzyme Linked ImmunoSorbent Assay'''''
−
|a3=
+
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
−
*Adsorption of antigen/antibody onto solid phase
+
|-
−
*Addition of chosen sample and reagents
+
|<big>'''What type of ELISA requires all the reagents to be added at once?'''
−
*Incubation and washing
+
||<font color="white"> <big>
−
*Addition of enzyme-labelled antigen/antibody
+
*'''''Homogenous'''''
−
*Addition of specific substrate
+
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
−
|l3=ELISA testing
+
|-
−
</FlashCard>
+
|<big>'''What are the five basic steps of an ELISA test?'''
 +
||<font color="white"> <big>
 +
*'''''Adsorption of antigen/antibody onto solid phase'''''
 +
*'''''Addition of chosen sample and reagents'''''
 +
*'''''Incubation and washing'''''
 +
*'''''Addition of enzyme-labelled antigen/antibody'''''
 +
*'''''Addition of specific substrate'''''
 +
||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
  
 +
|}
 
==<font color="purple">Immunofluorescence</font>==
 
==<font color="purple">Immunofluorescence</font>==
−
<FlashCard questions="4">
+
{| border="3" cellpadding="8"
−
|q1=The adsorption of light by a molecule is known as what?
+
!width="400"|'''Question'''
−
|a1=Excitation
+
!width="400"|'''Answer'''
−
|l1=Immunofluorescence
+
!width="150"|'''Article'''
−
|q2=What are the fluorescent dyes commonly known as?
+
|-
−
|a2=Fluorochromes
+
|<big>'''The adsorption of light by a molecule is known as what?'''
−
|l2=Immunofluorescence
+
||<font color="white"> <big>
−
|q3=What are the advantages of indirect staining?
+
*'''''Excitation'''''
−
|a3=
+
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
−
*Brighter fluorescence
+
|-
−
*One preparation can be used to test many serum samples
+
|<big>'''What are the fluorescent dyes commonly known as?'''
−
*Relative expressions of different antigens in the same cell can be tested
+
||<font color="white"> <big>
−
*Loss of antibody during conjugation is limited
+
*'''''Fluorochromes'''''
−
|l3=Immunofluorescence
+
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
−
|q4=What technique is used to provide a quantitative measure of immunofluorescence?
+
|-
−
|a4=Flow cytometry
+
|<big>'''What are the advantages of indirect staining?'''
−
|l4=Immunofluorescence
+
||<font color="white"> <big>
−
</FlashCard>
+
*'''''Brighter fluorescence'''''
 +
*'''''One preparation can be used to test many serum samples'''''
 +
*'''''Relative expressions of different antigens in the same cell can be tested'''''
 +
*'''''Loss of antibody during conjugation is limited'''''
 +
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
 +
|-
 +
|<big>'''What technique is used to provide a quantitative measure of immunofluorescence?'''
 +
||<font color="white"> <big>
 +
*'''''Flow cytometry'''''
 +
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
 +
|}
  
 
==<font color="purple">Radioimmunoassay</font>==
 
==<font color="purple">Radioimmunoassay</font>==
−
<FlashCard questions="4">
+
{| border="3" cellpadding="8"
−
|q1=What are radioimmunoassays commonly used to detect?
+
!width="400"|'''Question'''
−
|a2=
+
!width="400"|'''Answer'''
−
*Hormone levels in blood and tissue fluids
+
!width="150"|'''Article'''
−
*Serum proteins
+
|-
−
*Drugs
+
|<big>'''What are radioimmunoassays commonly used to detect?'''
−
*Vitamins
+
||<font color="white"> <big>
−
|l1=Radioimmunoassay
+
*'''''Hormone levels in blood and tissue fluids'''''
−
|q2=What is the most common isotope used for radioimmunoassay?
+
*'''''Serum proteins'''''
−
|a2=Iodine-125
+
*'''''Drugs'''''
−
|l2=Radioimmunoassay
+
*'''''Vitamins'''''
−
|q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
+
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
−
|a3=Ensures the number of epitopes present exceeds number of antibody-binding sites
+
|-
−
|l3=Radioimmunoassay
+
|<big>'''What is the most common isotope used for radioimmunoassay?'''
−
|q4=What are the disadvantages of the radioimmunoassay?
+
||<font color="white"> <big>
−
|a4=
+
*'''''Iodine-125'''''
−
*Substances used are radioactive
+
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
−
*Gamma radiation requires special counting equipment
+
|-
−
*Iodine naturally accumulates in the thyroid gland
+
|<big>'''Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?'''
−
|l4=Radioimmunoassay
+
||<font color="white"> <big>
−
</FlashCard>
+
*'''''Ensures the number of epitopes present exceeds number of antibody-binding sites'''''
 +
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
 +
|-
 +
|<big>'''What are the disadvantages of the radioimmunoassay?'''
 +
||<font color="white"> <big>
 +
*'''''Substances used are radioactive'''''
 +
*'''''Gamma radiation requires special counting equipment'''''
 +
*'''''Iodine naturally accumulates in the thyroid gland'''''
 +
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
  
 +
|}
 
==<font color="purple">Agglutination</font>==
 
==<font color="purple">Agglutination</font>==
−
<FlashCard questions="5">
+
{| border="3" cellpadding="8"
−
|q1=What are the antibodies that produce agglutination known as?
+
!width="400"|'''Question'''
−
|a1=Agglutinins
+
!width="400"|'''Answer'''
−
|l1=Agglutination
+
!width="150"|'''Article'''
−
|q2=What constitutes the end of the agglutination test?
+
|-
−
|a2=The last tube showing distinct agglutination
+
|<big>'''What are the antibodies that produce agglutination known as?'''
−
|l2=Agglutination
+
||<font color="white"> <big>
−
|q3=What does the titre represent?
+
*'''''Agglutinins'''''
−
|a3=The number of antibodies per unit volume of serum
+
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
−
|l3=Agglutination
+
|-
−
|q4=What is the prozone effect?
+
|<big>'''What constitutes the end of the agglutination test?'''
−
|a4=An inhibition of agglutination caused by an excess of antibodies
+
||<font color="white"> <big>
−
|l4=Agglutination
+
*'''''The last tube showing distinct agglutination'''''
−
|q5=What agglutination test is often used to test for rhesus disease?
+
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
−
|a5=Coombs test
+
|-
−
|l5=Agglutination
+
|<big>'''What does the titre represent?'''
−
</FlashCard>
+
||<font color="white"> <big>
 +
*'''''The number of antibodies per unit volume of serum'''''
 +
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 +
|-
 +
|<big>'''What is the prozone effect?'''
 +
||<font color="white"> <big>
 +
*'''''An inhibition of agglutination caused by an excess of antibodies'''''
 +
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 +
|-
 +
|<big>'''What agglutination test is often used to test for rhesus disease?'''
 +
||<font color="white"> <big>
 +
*'''''Coombs test'''''
 +
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
  
−
 
−
<br><br>
 
−
{{Jim Bee 2007}}
 
 
[[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]]
 
[[Category:Immunological Testing|Z]][[Category:Immunology Flashcards]]

Revision as of 14:53, 7 February 2011

ELISA testing

Question Answer Article
What does the acronym ELISA stand for?
  • Enzyme Linked ImmunoSorbent Assay
 Link to Answer Article
What type of ELISA requires all the reagents to be added at once?
  • Homogenous
 Link to Answer Article
What are the five basic steps of an ELISA test?
  • Adsorption of antigen/antibody onto solid phase
  • Addition of chosen sample and reagents
  • Incubation and washing
  • Addition of enzyme-labelled antigen/antibody
  • Addition of specific substrate
 Link to Answer Article

Immunofluorescence

Question Answer Article
The adsorption of light by a molecule is known as what?
  • Excitation
 Link to Answer Article
What are the fluorescent dyes commonly known as?
  • Fluorochromes
 Link to Answer Article
What are the advantages of indirect staining?
  • Brighter fluorescence
  • One preparation can be used to test many serum samples
  • Relative expressions of different antigens in the same cell can be tested
  • Loss of antibody during conjugation is limited
 Link to Answer Article
What technique is used to provide a quantitative measure of immunofluorescence?
  • Flow cytometry
 Link to Answer Article

Radioimmunoassay

Question Answer Article
What are radioimmunoassays commonly used to detect?
  • Hormone levels in blood and tissue fluids
  • Serum proteins
  • Drugs
  • Vitamins
 Link to Answer Article
What is the most common isotope used for radioimmunoassay?
  • Iodine-125
 Link to Answer Article
Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
  • Ensures the number of epitopes present exceeds number of antibody-binding sites
 Link to Answer Article
What are the disadvantages of the radioimmunoassay?
  • Substances used are radioactive
  • Gamma radiation requires special counting equipment
  • Iodine naturally accumulates in the thyroid gland
 Link to Answer Article

Agglutination

Question Answer Article
What are the antibodies that produce agglutination known as?
  • Agglutinins
 Link to Answer Article
What constitutes the end of the agglutination test?
  • The last tube showing distinct agglutination
 Link to Answer Article
What does the titre represent?
  • The number of antibodies per unit volume of serum
 Link to Answer Article
What is the prozone effect?
  • An inhibition of agglutination caused by an excess of antibodies
 Link to Answer Article
What agglutination test is often used to test for rhesus disease?
  • Coombs test
 Link to Answer Article
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