Difference between revisions of "Immunological Testing Flashcards"

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==<font color="purple">Immunofluorescence</font>==
 
==<font color="purple">Immunofluorescence</font>==
−
<FlashCard questions="4">
+
{| border="3" cellpadding="8"
−
|q1=The adsorption of light by a molecule is known as what?
+
!width="400"|'''Question'''
−
|a1=Excitation
+
!width="400"|'''Answer'''
−
|l1=Immunofluorescence
+
!width="150"|'''Article'''
−
|q2=What are the fluorescent dyes commonly known as?
+
|-
−
|a2=Fluorochromes
+
|<big>'''The adsorption of light by a molecule is known as what?'''
−
|l2=Immunofluorescence
+
||<font color="white"> <big>
−
|q3=What are the advantages of indirect staining?
+
*'''''Excitation'''''
−
|a3=
+
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
−
*Brighter fluorescence
+
|-
−
*One preparation can be used to test many serum samples
+
|<big>'''What are the fluorescent dyes commonly known as?'''
−
*Relative expressions of different antigens in the same cell can be tested
+
||<font color="white"> <big>
−
*Loss of antibody during conjugation is limited
+
*'''''Fluorochromes'''''
−
|l3=Immunofluorescence
+
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
−
|q4=What technique is used to provide a quantitative measure of immunofluorescence?
+
|-
−
|a4=Flow cytometry
+
|<big>'''What are the advantages of indirect staining?'''
−
|l4=Immunofluorescence
+
||<font color="white"> <big>
−
</FlashCard>
+
*'''''Brighter fluorescence'''''
 +
*'''''One preparation can be used to test many serum samples'''''
 +
*'''''Relative expressions of different antigens in the same cell can be tested'''''
 +
*'''''Loss of antibody during conjugation is limited'''''
 +
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
 +
|-
 +
|<big>'''What technique is used to provide a quantitative measure of immunofluorescence?'''
 +
||<font color="white"> <big>
 +
*'''''Flow cytometry'''''
 +
||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]]
 +
|}
  
 
==<font color="purple">Radioimmunoassay</font>==
 
==<font color="purple">Radioimmunoassay</font>==
−
<FlashCard questions="4">
+
{| border="3" cellpadding="8"
−
|q1=What are radioimmunoassays commonly used to detect?
+
!width="400"|'''Question'''
−
|a2=
+
!width="400"|'''Answer'''
−
*Hormone levels in blood and tissue fluids
+
!width="150"|'''Article'''
−
*Serum proteins
+
|-
−
*Drugs
+
|<big>'''What are radioimmunoassays commonly used to detect?'''
−
*Vitamins
+
||<font color="white"> <big>
−
|l1=Radioimmunoassay
+
*'''''Hormone levels in blood and tissue fluids'''''
−
|q2=What is the most common isotope used for radioimmunoassay?
+
*'''''Serum proteins'''''
−
|a2=Iodine-125
+
*'''''Drugs'''''
−
|l2=Radioimmunoassay
+
*'''''Vitamins'''''
−
|q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
+
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
−
|a3=Ensures the number of epitopes present exceeds number of antibody-binding sites
+
|-
−
|l3=Radioimmunoassay
+
|<big>'''What is the most common isotope used for radioimmunoassay?'''
−
|q4=What are the disadvantages of the radioimmunoassay?
+
||<font color="white"> <big>
−
|a4=
+
*'''''Iodine-125'''''
−
*Substances used are radioactive
+
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
−
*Gamma radiation requires special counting equipment
+
|-
−
*Iodine naturally accumulates in the thyroid gland
+
|<big>'''Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?'''
−
|l4=Radioimmunoassay
+
||<font color="white"> <big>
−
</FlashCard>
+
*'''''Ensures the number of epitopes present exceeds number of antibody-binding sites'''''
 +
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
 +
|-
 +
|<big>'''What are the disadvantages of the radioimmunoassay?'''
 +
||<font color="white"> <big>
 +
*'''''Substances used are radioactive'''''
 +
*'''''Gamma radiation requires special counting equipment'''''
 +
*'''''Iodine naturally accumulates in the thyroid gland'''''
 +
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
  
 +
|}
 
==<font color="purple">Agglutination</font>==
 
==<font color="purple">Agglutination</font>==
−
<FlashCard questions="5">
+
{| border="3" cellpadding="8"
−
|q1=What are the antibodies that produce agglutination known as?
+
!width="400"|'''Question'''
−
|a1=Agglutinins
+
!width="400"|'''Answer'''
−
|l1=Agglutination
+
!width="150"|'''Article'''
−
|q2=What constitutes the end of the agglutination test?
+
|-
−
|a2=The last tube showing distinct agglutination
+
|<big>'''What are the antibodies that produce agglutination known as?'''
−
|l2=Agglutination
+
||<font color="white"> <big>
−
|q3=What does the titre represent?
+
*'''''Agglutinins'''''
−
|a3=The number of antibodies per unit volume of serum
+
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
−
|l3=Agglutination
+
|-
−
|q4=What is the prozone effect?
+
|<big>'''What constitutes the end of the agglutination test?'''
−
|a4=An inhibition of agglutination caused by an excess of antibodies
+
||<font color="white"> <big>
−
|l4=Agglutination
+
*'''''The last tube showing distinct agglutination'''''
−
|q5=What agglutination test is often used to test for rhesus disease?
+
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
−
|a5=Coombs test
+
|-
−
|l5=Agglutination
+
|<big>'''What does the titre represent?'''
−
</FlashCard>
+
||<font color="white"> <big>
 +
*'''''The number of antibodies per unit volume of serum'''''
 +
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 +
|-
 +
|<big>'''What is the prozone effect?'''
 +
||<font color="white"> <big>
 +
*'''''An inhibition of agglutination caused by an excess of antibodies'''''
 +
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 +
|-
 +
|<big>'''What agglutination test is often used to test for rhesus disease?'''
 +
||<font color="white"> <big>
 +
*'''''Coombs test'''''
 +
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
  
  

Revision as of 17:00, 17 March 2012

ELISA testing

Question Answer Article
What does the acronym ELISA stand for? Link to Article
What type of ELISA requires all the reagents to be added at once? Link to Article
What are the five basic steps of an ELISA test? Link to Article


Immunofluorescence

Question Answer Article
The adsorption of light by a molecule is known as what?
  • Excitation
 Link to Answer Article
What are the fluorescent dyes commonly known as?
  • Fluorochromes
 Link to Answer Article
What are the advantages of indirect staining?
  • Brighter fluorescence
  • One preparation can be used to test many serum samples
  • Relative expressions of different antigens in the same cell can be tested
  • Loss of antibody during conjugation is limited
 Link to Answer Article
What technique is used to provide a quantitative measure of immunofluorescence?
  • Flow cytometry
 Link to Answer Article

Radioimmunoassay

Question Answer Article
What are radioimmunoassays commonly used to detect?
  • Hormone levels in blood and tissue fluids
  • Serum proteins
  • Drugs
  • Vitamins
 Link to Answer Article
What is the most common isotope used for radioimmunoassay?
  • Iodine-125
 Link to Answer Article
Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
  • Ensures the number of epitopes present exceeds number of antibody-binding sites
 Link to Answer Article
What are the disadvantages of the radioimmunoassay?
  • Substances used are radioactive
  • Gamma radiation requires special counting equipment
  • Iodine naturally accumulates in the thyroid gland
 Link to Answer Article

Agglutination

Question Answer Article
What are the antibodies that produce agglutination known as?
  • Agglutinins
 Link to Answer Article
What constitutes the end of the agglutination test?
  • The last tube showing distinct agglutination
 Link to Answer Article
What does the titre represent?
  • The number of antibodies per unit volume of serum
 Link to Answer Article
What is the prozone effect?
  • An inhibition of agglutination caused by an excess of antibodies
 Link to Answer Article
What agglutination test is often used to test for rhesus disease?
  • Coombs test
 Link to Answer Article




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