Difference between revisions of "Immunity to Parasites"

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==Innate Immunity==
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The first line of defence against parasitic infection are the effector mechanisms of the innate immune system:
[[File:Helminth Killing edit.png|300px|right|thumb|Helminth Killing - R.J.Francis, RVC 2012]]
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*'''Macrophages'''- important in the defence against extracellular parasites, macrophages are able to secrete cytokines as well as perform phagocytosis
[[File:Helmith Killing by Granulocytes.png|250px|right|thumb|Electron Micrograph of Helminth Killing by Granulocytes (G). Adapted from [[http://eprints.adm.unipi.it/527/]] - R.J.Francis, RVC 2012]]
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**Can act as 'killer cells' through antibody-dependent cell-mediated cytotoxicity, e.g. specific [[IgG]]/[[IgE]] enhances the ability of macrophages to kill schistosomules
The first line of defence against parasitic infection are the effector mechanisms of the innate immune system. The '''[[Macrophages|macrophages]]''' are important players in the defence against extracellular parasites. This is because macrophages are able to secrete [[Cytokines|cytokines]] as well as perform [[Phagocytosis|phagocytosis]]. In this they can act as 'killer cells' through antibody-dependent cell-mediated cytotoxicity, for example specific [[Immunoglobulins|IgG]]/[[Immunoglobulins|IgE]] enhances the ability of macrophages to kill schistosomules through the interaction of Fc receptors on the surface of the macrophage. Of the secreted cytokines, the secretion of TNFα is of particular importance. This is because TNFα activates other macrophages and can have toxic effects in high amounts. TNFα also renders hepatocytes resistant to malarial infection when in conjunction with IL-1. Cytokine secretion (in particular IFNγ) can also enhance killing by mechanisms using free radicals and O<sub>2</sub>-independent toxins (e.g. nitric oxide).
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**The secretion of TNF-alpha:
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***Activates other macrophages
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***Renders hepatocytes resistant to malarial infection when in conjunction with IL-1
As well as the macrophages, the granulocytes form the main effector response to parasitic infection. '''[[Eosinophils|Eosinophils]]''' are very important in the  destruction of larger parasites even though they are less phagocytic than [[Neutrophils|neutrophils]]. Most activity from eosinophils is controlled by antigen-specific mechanisms, for example binding to worms coated with [[IgG]]/[[IgE]] increases '''degranulation'''. Degranulation, through the process known as '''exocytosis''', releases enzymes that degrade the parasite into smaller chunks so it can be cleared by phagocytosis. The eosinophils also form the end point of the adaptive immune response to larger parasites with the killing of some larvae being enhanced by the activity of mast cells, for example antigens released by ''S. mansoni'' cause IgE-dependent degranulation of mast cells, the products of which selectively attract eosinophils. Mast cells and eosinophils degranulation enhances the T<sub>H</sub>2 response through the release of [[Cytokines|cytokines]], for example IL-4 and IL-13, that is needed to clear a stubborn infection.
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***Can have toxic effects in high amounts
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**When activated by cytokines, killing mechanisms using free radicals and O2-independent toxins (e.g. nitric oxide) are enhanced
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*'''[[Neutrophils|Neutrophils]]'''- similar properties to macrophages
The other granulocytes present in parasite-infected inflammatory lesions and involved in the anti-parasitic response are the '''[[Neutrophils|neutrophils]]'''. In the parasitic response they have similar properties to macrophages with activation caused by cytokines such as TNFα, IFNγ and GM-CSF. Their mechanism for killing is by an intense respiratory burst, with extracellular killing being mediated by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). In addition, they also express Fc and [[Complement|complement]] receptors so can participate in antibody-dependent cell-mediated cytotoxicity and [[Phagocytosis|phagocytosis]]. Due to innate inflammatory mechanisms neutrophils are early responders to parasite infection.
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**Activated by cytokines such as TNF-alpha, IFN-γ and GM-CSF, [[[[Neutrophils|Neutrophils]] - WikiBlood|neutrophils]] produce a more intense respiratory burst and extracellular killing is mediated by H2O2
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**Present in parasite-infected inflammatory lesions
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**Express Fc and complement receptors- can participate in antibody-dependent cell-mediated cytotoxicity
'''[[Platelets|Platelets]]''' also form part of the innate immune response to parasites. Their main mode of action is the binding and release of granular contents, in particular inflammatory peptides. The cytotoxic activity of plateltes is increased by cytokines such as TNFα and IFNγ. The potential targets include the larval stage of flukes, e.g. ''T. gondii'', ''T. cruzi'' and ''S. Mansoni''. Like other effector cells, platelets express Fc receptors, making them able to perform antibody-dependent cytotoxicity.
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*'''Eosinophils'''- less phagocytic than [[[[Neutrophils|Neutrophils]] - WikiBlood|neutrophils]], but important in the destruction of larger parasites
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**Most activity is controlled by antigen-specific mechanisms, e.g. binding to worms coated with [[IgG]]/[[IgE]] increases degranulation
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**The killing of some larvae is enhanced by the activity of mast cells, e.g. antigens released by S. mansoni cause [[IgE]]-dependent degranulation of mast cells, the products of which selectively attract eosinophils
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*'''Platelets'''- cytotoxic activity is increased by cytokines such as TNF-alpha and IFN-γ
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**Potential targets include the larval stage of flukes, e.g. ''T. gondii'' and ''T. cruzi''
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**Like other effector cells, platelets express Fc receptors, making them able to perform antibody-dependent cytotoxicity
  
==Adaptive Immunity==
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Although the innate immune system provides an effective first line of defence, T cells are fundamental in the development of immunity, demonstrated using T-cell deprived mice that fail to resolve otherwise non-lethal infections of, for example, ''T. cruzi''.  
Although the innate immune system provides an effective first line of defence, '''[[T cells|T cells]]''' are fundamental in the development of immunity, demonstrated using T-cell deprived mice that fail to resolve otherwise non-lethal infections of, for example, ''T. cruzi''.  
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*Both CD4+ and CD8+ cells are required for protection, e.g CD4+ cells protect against the blood stage of a Plasmodium infection (erythrocytes do not express MHC class I), while CD8+ cells are required to mediate immunity against the liver stage (hepatocytes do not express MHC class II).
*Both CD4<sup>+</sup> and CD8<sup>+</sup> cells are required for protection, e.g CD4<sup>+</sup> cells protect against the blood stage of a Plasmodium infection (erythrocytes do not express MHC class I), while CD8<sup>+</sup> cells are required to mediate immunity against the liver stage (hepatocytes do not express MHC class II).
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*Th1 cells are required to fight intracellular protozoa- the release of IFN-γ activates macrophages to kill the protozoa residing within them
*T<sub>H</sub>1 cells are required to fight intracellular protozoa - the release of IFNγ activates macrophages to kill the protozoa residing within them
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*Helminth infections require both Th1 and Th2 responses, e.g. during ''S. mansoni'' the secretion of IFN-γ by Th1 cells activates mechanisms that destroy larvae in the lungs, although the Th2 subset, secreting IL-5, predominate
*Helminth infections require both T<sub>H</sub>1 and T<sub>H</sub>2 responses, e.g. during ''S. mansoni'' the secretion of IFNγ by T<sub>H</sub>1 cells activates mechanisms that destroy larvae in the lungs, although the T<sub>H</sub>2 subset, secreting IL-5, predominate. IL-5 is responsible for the eosinophilia associated with parasite infections.
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*Th2 cells are required for the destruction of intestinal worms, where they induce mucosal mast cells and interact with eosinophils
*T<sub>H</sub>2 cells are required for the destruction of intestinal worms, where they induce mucosal mast cells and interact with [[Eosinophils|eosinophils]]
 
 
While cell-mediated immunity is important in tissue infections, such as Leishmania, specific antibodies are important in controlling parasites that live in the bloodstream, e.g. malaria. Mechanisms of antibody-mediated immunity include:
 
While cell-mediated immunity is important in tissue infections, such as Leishmania, specific antibodies are important in controlling parasites that live in the bloodstream, e.g. malaria. Mechanisms of antibody-mediated immunity include:
 
*Directly damaging protozoa
 
*Directly damaging protozoa
*Activating [[Complement|complement]], and the subsequent Membrane Attack Complex
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*Activating complement
 
*Blocking attachment to host cells
 
*Blocking attachment to host cells
*Enhancing macrophage [[Phagocytosis|phagocytosis]]
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*Enhancing macrophage phagocytosis
 
*Involvement in antibody-dependent cell-mediated cytotoxicity
 
*Involvement in antibody-dependent cell-mediated cytotoxicity
  
=='''Immunopathology'''==
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'''Immunopathology'''
The immunopathology is the damage resulting from an immune response to the parasites. Examples include:
 
 
*The increase in macrophages and lymphocytes in the liver and spleen can lead to swelling of these organs, e.g. visceral leishmaniasis
 
*The increase in macrophages and lymphocytes in the liver and spleen can lead to swelling of these organs, e.g. visceral leishmaniasis
*T-cell dependent (T<sub>H</sub>1 and T<sub>H</sub>17) granulomas forming in organs, e.g. schistosomiasis in the liver
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*T-cell dependent granulomas forming in organs, e.g. schistosomiasis in the liver
 
*The pathology of elephantiasis is thought to be due to changes in the adult filariae in the lymphatic system
 
*The pathology of elephantiasis is thought to be due to changes in the adult filariae in the lymphatic system
 
*Formation of immune complexes, e.g. deposition in the kidney during malarial infection
 
*Formation of immune complexes, e.g. deposition in the kidney during malarial infection
 
*Anaphylactic shock caused by [[IgE]] production, e.g. after the rupture of hydatid cysts
 
*Anaphylactic shock caused by [[IgE]] production, e.g. after the rupture of hydatid cysts
 
*Cross-reaction of antibodies with host tissue, e.g. ''O. volvulus'', the cause of river blindness, expresses an antigen similar to a protein in the retina
 
*Cross-reaction of antibodies with host tissue, e.g. ''O. volvulus'', the cause of river blindness, expresses an antigen similar to a protein in the retina
*Excessive production of cytokines, such as TNFα, may contribute to pathology of diseases such as malaria
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*Excessive production of cytokines, such as TNF-alpha, may contribute to pathology of diseases such as malaria
  
=='''Evading immune defences'''==
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'''Evading immune defences''':
  
 
Parasites can evade an immune response from the host by changing the antigens presented to the host, produce antigens that mimic the host's antigens and can produce down-regulating factors which suppress or modify the host's immune responses. Having a rapid turnover of their surface coat when host cells bind and by being able to live in sites which are protected from the host's immune response allow parasites to establish themselves in a particular species.
 
Parasites can evade an immune response from the host by changing the antigens presented to the host, produce antigens that mimic the host's antigens and can produce down-regulating factors which suppress or modify the host's immune responses. Having a rapid turnover of their surface coat when host cells bind and by being able to live in sites which are protected from the host's immune response allow parasites to establish themselves in a particular species.
*Selection of innapropriate defences - by exploiting the 'adjuvant' mechanism, some parasites are able to activate the inappropriate helper T cell subset, e.g Leishmania
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*Selection of innapropriate defences- by exploiting the 'adjuvant' mechanism, some parasites are able to activate the inappropriate helper T cell subset, e.g Leishmania
 
*Antigenic variation avoids recognition by antibody and complement, e.g. ''T. brucei''
 
*Antigenic variation avoids recognition by antibody and complement, e.g. ''T. brucei''
 
*Inhibiting fusion of lysosomes
 
*Inhibiting fusion of lysosomes
 
*Escaping into the cytoplasm, e.g. ''T. cruzi''
 
*Escaping into the cytoplasm, e.g. ''T. cruzi''
 
*Inhibiting respiratory burst, e.g. Leishmania
 
*Inhibiting respiratory burst, e.g. Leishmania
*Forming cysts in muscle tissue, e.g. ''T. spiralis'' - also develops decay accelerating factor (DAF)
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*Forming cysts in muscle tissue, e.g. ''T. spiralis''- also develops decay accelerating factor (DAF)
 
*Production of antioxidants, e.g. ''W. bancrofti''
 
*Production of antioxidants, e.g. ''W. bancrofti''
 
<big>'''Also see [[Adaptive Immunity to Parasites]]'''</big>
 
  
 
[[Category:Parasites]]
 
[[Category:Parasites]]
 
[[Category:To_Do_-_Blood]]
 

Revision as of 16:31, 12 June 2010

The first line of defence against parasitic infection are the effector mechanisms of the innate immune system:

  • Macrophages- important in the defence against extracellular parasites, macrophages are able to secrete cytokines as well as perform phagocytosis
    • Can act as 'killer cells' through antibody-dependent cell-mediated cytotoxicity, e.g. specific IgG/IgE enhances the ability of macrophages to kill schistosomules
    • The secretion of TNF-alpha:
      • Activates other macrophages
      • Renders hepatocytes resistant to malarial infection when in conjunction with IL-1
      • Can have toxic effects in high amounts
    • When activated by cytokines, killing mechanisms using free radicals and O2-independent toxins (e.g. nitric oxide) are enhanced
  • Neutrophils- similar properties to macrophages
    • Activated by cytokines such as TNF-alpha, IFN-γ and GM-CSF, [[Neutrophils - WikiBlood|neutrophils]] produce a more intense respiratory burst and extracellular killing is mediated by H2O2
    • Present in parasite-infected inflammatory lesions
    • Express Fc and complement receptors- can participate in antibody-dependent cell-mediated cytotoxicity
  • Eosinophils- less phagocytic than [[Neutrophils - WikiBlood|neutrophils]], but important in the destruction of larger parasites
    • Most activity is controlled by antigen-specific mechanisms, e.g. binding to worms coated with IgG/IgE increases degranulation
    • The killing of some larvae is enhanced by the activity of mast cells, e.g. antigens released by S. mansoni cause IgE-dependent degranulation of mast cells, the products of which selectively attract eosinophils
  • Platelets- cytotoxic activity is increased by cytokines such as TNF-alpha and IFN-γ
    • Potential targets include the larval stage of flukes, e.g. T. gondii and T. cruzi
    • Like other effector cells, platelets express Fc receptors, making them able to perform antibody-dependent cytotoxicity

Although the innate immune system provides an effective first line of defence, T cells are fundamental in the development of immunity, demonstrated using T-cell deprived mice that fail to resolve otherwise non-lethal infections of, for example, T. cruzi.

  • Both CD4+ and CD8+ cells are required for protection, e.g CD4+ cells protect against the blood stage of a Plasmodium infection (erythrocytes do not express MHC class I), while CD8+ cells are required to mediate immunity against the liver stage (hepatocytes do not express MHC class II).
  • Th1 cells are required to fight intracellular protozoa- the release of IFN-γ activates macrophages to kill the protozoa residing within them
  • Helminth infections require both Th1 and Th2 responses, e.g. during S. mansoni the secretion of IFN-γ by Th1 cells activates mechanisms that destroy larvae in the lungs, although the Th2 subset, secreting IL-5, predominate
  • Th2 cells are required for the destruction of intestinal worms, where they induce mucosal mast cells and interact with eosinophils

While cell-mediated immunity is important in tissue infections, such as Leishmania, specific antibodies are important in controlling parasites that live in the bloodstream, e.g. malaria. Mechanisms of antibody-mediated immunity include:

  • Directly damaging protozoa
  • Activating complement
  • Blocking attachment to host cells
  • Enhancing macrophage phagocytosis
  • Involvement in antibody-dependent cell-mediated cytotoxicity

Immunopathology

  • The increase in macrophages and lymphocytes in the liver and spleen can lead to swelling of these organs, e.g. visceral leishmaniasis
  • T-cell dependent granulomas forming in organs, e.g. schistosomiasis in the liver
  • The pathology of elephantiasis is thought to be due to changes in the adult filariae in the lymphatic system
  • Formation of immune complexes, e.g. deposition in the kidney during malarial infection
  • Anaphylactic shock caused by IgE production, e.g. after the rupture of hydatid cysts
  • Cross-reaction of antibodies with host tissue, e.g. O. volvulus, the cause of river blindness, expresses an antigen similar to a protein in the retina
  • Excessive production of cytokines, such as TNF-alpha, may contribute to pathology of diseases such as malaria

Evading immune defences:

Parasites can evade an immune response from the host by changing the antigens presented to the host, produce antigens that mimic the host's antigens and can produce down-regulating factors which suppress or modify the host's immune responses. Having a rapid turnover of their surface coat when host cells bind and by being able to live in sites which are protected from the host's immune response allow parasites to establish themselves in a particular species.

  • Selection of innapropriate defences- by exploiting the 'adjuvant' mechanism, some parasites are able to activate the inappropriate helper T cell subset, e.g Leishmania
  • Antigenic variation avoids recognition by antibody and complement, e.g. T. brucei
  • Inhibiting fusion of lysosomes
  • Escaping into the cytoplasm, e.g. T. cruzi
  • Inhibiting respiratory burst, e.g. Leishmania
  • Forming cysts in muscle tissue, e.g. T. spiralis- also develops decay accelerating factor (DAF)
  • Production of antioxidants, e.g. W. bancrofti