Difference between revisions of "Equine Togaviral Encephalitis"
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+ | Also known as: '''''Alphaviral encephalitis — Alphaviral encephalitides — Eastern equine encephalitis — Eastern equine encephalomyelitis — EEE — Western equine encephalitis — Western equine encephalomyelitis — WEE — Venezuelan equine encephalitis — Venezuelan equine encephalomyelitis — VEE | ||
− | + | ==Introduction== | |
− | + | '''NOTIFIABLE''' and '''ZOONOTIC''' OIE List B, infectious, mosquito-borne diseases of equidae affecting the central nervous system (CNS). They include: | |
− | + | *Eastern Equine Encephalitis (EEE) | |
− | ''' | + | *Western Equine Encephalitis (WEE) |
− | ''' | + | *Venezuelan Equine Encephalitis (VEE) - '''reportable''' in the USA<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> |
− | ''' | ||
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+ | ==Aetiology== | ||
+ | See this page for details of the [[Equine Encephalitis Virus|equine encephalitis causal pathogens]]. Some of the virus strains can infect swine<ref>Karsted, L, Hansen, R.P (1959) Natural and experimental infections in swine with the virus of eastern equine encephalomyelitis, ''J Infect Dis'' 105:293-296. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>, poultry and other farmed birds including quail and ratites.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Isolated cases have also been noted in cattle<ref>Pursell, A.R, Mitchell, F.E, Seibold, H.R (1976) Naturally occurring and experimentally induced eastern encephalomyelitis in calves, ''J Am Vet Med Assoc'', 169:1101-1103. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>, sheep and non-domestic ungulates.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Some strains are potential agents of biowarfare or bioterrorism<ref>Steele, K.E, Twenhafel, N.A (2010) Review Paper: Pathology of Animal Models of Alphavirus Encephalitis. ''Vet Pathol.'' Jun 15. [Epub ahead of print].</ref>. | ||
− | ==== | + | ==Epidemiology== |
− | ''' | + | ===Distribution=== |
+ | Togaviral encephalitis in equids is largely confined to the Western Hemisphere.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Venezuelan EEV can cause large outbreaks of disease over extensive geographical areas in both humans and horses. Spread of this virus into Central America has had disasterous consequences with epidemics as far north as Texas.<ref name="duplicate">http://www.defra.gov.uk/foodfarm/farmanimal/diseases/atoz/viralenceph/index.htm, accessed July 2010</ref> Climatic conditions and interventions that support vector populations, such as irrigation, greatly influence the geographical spread of the disease.<ref name="duplicate">http://www.defra.gov.uk/foodfarm/farmanimal/diseases/atoz/viralenceph/index.htm, accessed July 2010</ref> EEE has been recorded across the United States, but mostly in the Southeastern States.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> As its names suggests, WEE has a predilection for the Western states which have been subject to significant outbreaks in the past. A regional alteration in virulence has been proposed for the steep decline in clinical case numbers observed in this area.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> A lag phase of 2-5weeks is commonly observed between horse and human cases of WEE in a given locus.<ref>McLintock, J (1980) The arbovirus problem in Canada, ''Can J Public Health'', 67(Suppl 1):8-12. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Both are dead-end hosts for the virus. A subtype of Western EEV, Highlands J virus, was isolated from the brain of a horse with encephalitis in Florida.<ref>Karabatsos, N, Lewis, A.L, Calisher, C.H, Hunt, A.R, and Roehrig, J.T (1988). Identification of Highland J virus from a Florida horse. ''Am J Trop Med Hyg'', 39, 603-606. In: ''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> | ||
− | === | + | ===Transmission=== |
− | [[ | + | Transfer is '''vector'''-mediated, primarily via '''[[Culicidae|mosquito]]''' salivary transfer. WEE and VEE may also be transmitted horse to horse through nasal secretions. This mode of transmission is less likely, despite the fact that high concentrations of VEE virus are found in ocular and nasal discharges from infected horses.<ref>Kissling, R.E, Chamberlain, R.W (1967) Venezuelan equine encephalitis, ''Adv Vet Sci Comp Med'', 11:65-84. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> The viraemic phase ends when nervous signs develop<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> and is important for disease amplification. Amplification from horses is likely only with VEE virus, in association with a relatively high and potentially persistent viraemia.<ref name="again">Merck & Co (2008) The Merck Veterinary Manual (Eighth Edition), Merial found at http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/100900.htm&word=Equine%2cencephalitis, accessed July 2010</ref> Similarly, zoonotic spread is unlikely for Eastern and Western equine encephalitis, but has been noted with VEE. |
− | === | + | ===Seasonal Incidence=== |
− | + | The disease is not directly contagious between horses and humans but occurs sporadically in both species from mid-summer to late autumn - during the height of the vector season.<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> Case numbers peak in June to November in temperate climates.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> The vector season is longer in warmer climates, where the disease period is prolonged. Global warming may promote more outbreaks in historically colder climates.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> | |
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− | ===== | + | ===Epidemics=== |
− | + | Outbeak prediction to date has been inaccurate, implying that other, unidentified factors may be in operation.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> However, some epidemic requirements are beyond question. Adequate amounts of infective virus, sufficent vectors, infected sylvatic hosts and susceptible terminal hosts, and finally, appropriate reservoirs, are all crucial.<ref>Sellers, R.F (1980) Weather, host and vector: their interplay in the spread of insect-borne animal virus diseases, ''J Hyg (Lond)'', 85:65-102. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> | |
− | === | + | ==Pathogenesis<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>== |
− | + | Upon entry to the host, viruses multiply in the muscle, enter the lymphatic circulation and localize in lymph nodes. In macrophages and neutrophils viral replication leads to shedding and significant clearance of viral particles. No further clinical signs develop if this clearance is successful. Erythrocyte and leukocyte absorption are used to circumvent the immune defences of the host. After incomplete elimination, residual virus infects endothelial cells and accumulates in highly vascular organs such as the liver and spleen. In these organs, viral replication produces circulating virus and a second viraemic period, typically associated with early clinical signs. Neuroinvasion and replication occurs within a week.<ref name="again">Merck & Co (2008) The Merck Veterinary Manual (Eighth Edition), Merial found at http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/100900.htm&word=Equine%2cencephalitis, accessed July 2010</ref> An incubation period of 7-21days has been demonstrated after experimental infection with Eastern or Western EEV, but the incubation is often shorter for EEE compared with that of WEE. | |
− | == | + | ==Signalment== |
− | + | Unvaccinated adult horses and other equids ([[Venezuelan Equine Encephalomyelitis (VEE) - Donkey|'''VEE in donkeys''']]) are at risk in areas with suitable vectors. Vaccinated horses can still develop the disease, particularly if they are young or old. | |
− | ==== | + | ==Clinical Signs== |
− | |||
− | + | Worse in unvaccinated horses.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Neurological signs may be assymmetrical.<ref name="again">Merck & Co (2008) The Merck Veterinary Manual (Eighth Edition), Merial found at http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/100900.htm&word=Equine%2cencephalitis, accessed July 2010</ref> | |
− | + | ===EEE and WEE=== | |
− | + | Following an incubation period of up to 21days<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>, an initial pyrexia and mild depression are short-lived and often missed. The acute phase of the disease presents with mild to severe pyrexia, anorexia and stiffness, lasting up to 5 days. During this time, the horse is viraemic and capable of amplifying the disease. The fever may then fluctuate with neurological derangements appearing a few days post-infection.<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> These changes indicate disease progression, which occurs more frequently with EEE (the most virulent of the three serotypes). Any of the following may be observed<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>: | |
− | + | *conscious proprioceptive deficits | |
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*propulsive walking | *propulsive walking | ||
*depression | *depression | ||
*somnolence | *somnolence | ||
− | * | + | *aggression |
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*excitability | *excitability | ||
− | * | + | *restlessness |
− | + | *hypersensitivity to sound and touch | |
− | + | Worsening neurological deficits may result in<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>: | |
*head pressing | *head pressing | ||
− | * | + | *head tilt |
− | * | + | *ataxia |
*circling | *circling | ||
− | * | + | *apparent blindness |
*facial and appendicular muscle fasciculations | *facial and appendicular muscle fasciculations | ||
− | * | + | *pendulous lower lip<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> |
− | *recumbency for 1-7 days followed by death | + | *pharynx, larynx and tongue paralysis |
− | VEE may | + | *seizures<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> |
− | *pyrexia peaks early and | + | *recumbency for 1-7 days followed by death<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> |
− | *mild | + | |
− | *severe pyrexia and leukopenia | + | ===VEE=== |
− | + | Clinical signs may resemble those of WEE and EEE or may vary<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>: | |
+ | *pyrexia peaks early and persists throughout the disease course | ||
+ | *mild pyrexia and leukopenia with endemic strains | ||
+ | *severe pyrexia and leukopenia with epidemic strains | ||
*neurological signs around 4 days post-infection | *neurological signs around 4 days post-infection | ||
− | *other | + | *diarrhoea, severe depression, recumbency and death may precede neurological signs |
− | + | *other signs: abortion, oral ulceration, pulmonary haemorrhage, epistaxis | |
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− | == | + | ==Diagnosis== |
− | + | Presumptive based on history, epidemiology and clinical signs.<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> Definitive diagnosis requires virus identification, serological tests and/or post-mortem examination. | |
− | + | ===Laboratory Tests=== | |
+ | ====[[Equine Encephalitis Virus#Virus Identification|Virus Identification]]==== | ||
− | + | ====Serology==== | |
− | + | Ab titre increases sharply within 24 hours of the initial viraemia, before clinical signs are apparent. It then deteriorates over 6 months. Samples taken when clinical signs appear are likely to miss the Ab peak and will demonstrate a decreasing titre.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Thus, serological confirmation of Eastern or Western EEV infection requires a four-fold or greater increase<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> OR decrease in Ab titre in paired serum samples taken 10-14 days apart.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> A presumptive diagnosis can be made on a single sample if an unvaccinated horse with suggestive clinical signs has Ab against only Eastern or Western EEV.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> Colostral-derived Ab has a serum half-life of around 20days and may interfere with diagnosis in foals.<ref name="repeat"> Ferguson, J.A, Reeves, W.C, Hardy, J.L (1979) Studies on immunity to alphaviruses in foals, ''Am J Vet Res'', 40:5-10. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> | |
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− | + | *Complement fixation (CF): to avoid anti-complementary effects, serum should be separated from blood as soon as possible.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> CF Ab against both Eastern and Western EEV is less useful for serological diagnosis because it appears relatively late and does not persist.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> | |
− | + | *Haemagglutination inhibition (HAI): titres of 1/10 and 1/20 are indicative, titres of 1/40 and above are positive.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> | |
− | + | *ELISA may be used to detect viral-specific IgM to the surface glycoprotein of Venezuelan EEV, from 3 days post-onset of clinical signs up to 21 days post-infection. This is useful in acute infections where convalescent serum samples are unobtainable.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> | |
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− | + | *The plaque reduction neutralization (PRN) test is very specific and can differentiate EEE and WEE infections. It is performed in duck embryo fibroblast, Vero, or BHK-21 cell cultures. Serum is tested against 100 plaque-forming units of virus. Endpoints are based on a 90% reduction in the number of plaques compared with the virus control.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> | |
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− | = | + | Most commonly, the PRN test or a combination of PRN and HAI tests is used to detect Ab against Eastern and Western EEV. Cross-reactivity occurs between Ab against these viruses in the CF and HAI tests.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> |
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− | ==== | + | ====Clinical Pathology==== |
− | + | CSF samples demonstrate increased cellularity (50-400 mononuclear cells/µl) and protein concentration (100-200mg/dl).<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> | |
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− | + | ===Post-mortem findings=== | |
− | + | '''''PRECAUTION'': infective viral particles may be present in CNS and other tissues'''.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> | |
− | Gross pathological lesions are rarely | + | Gross pathological lesions of the brain and spinal cord are rarely seen in horses, although traumatic ecchymotic haemorrhages and vascular congestion of the CNS may be evident.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> The extent of microscopic lesions is dictated by the severity of infection and duration of neurological involvement.<ref>Walton T.E (1981) Venezuelan, eastern, and western encephalomyelitis. In: ''Virus Diseases of Food Animals. A World Geography of Epidemiology and Control''. Disease Monographs, Vol. 2, Gibbs E.P.J, ed. Academic Press, New York, USA, 587-625.</ref> Such lesions with or without immunohistochemistry may be diagnostic.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> The cerebral cortex, thalamus and hypothalamus are often severely affected. Mononuclear meningitis, neuronal degeneration, diffuse and focal gliosis and perivascular cuffing are also seen.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> Histological lesions of EEE are usually present throughout the CNS, with widespread and severe neutrophilic inflammation of the grey matter. Lesions caused by Western EEV infection are more focal and lymphocytic in nature.<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> VEE cases often exhibit haemorrhage and liquefactive necrosis of the cerebral cortex, but lesions are not restricted to the CNS. In the pancreas, acinar cells atrophy and duct cells undergo hyperplasia.<ref>Monlux, W.S, Luedke, A.J (1973) Brain and spinal cord lesions in horses inoculated with Venezuelan equine encephalomyelitis virus (epidemic American and Trinidad strains), ''Am J Vet Res'', 34:465-473. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> There may also be damage to the liver and heart.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> |
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[[Image:Venezuelan_equine_encephalitis_virus.jpg|300px|thumb|right|''' (Photomicrograph of mouse brain tissue after dying of Venezuelan Encephalitis. Reveals neural necrosis and edema. ''Sourced from Wikimedia Commons, Copyright of the Centers for Disease Control and Prevention (CDC) Public Health Image Library (PHIL) Image #2809, 2006)'' ''']] | [[Image:Venezuelan_equine_encephalitis_virus.jpg|300px|thumb|right|''' (Photomicrograph of mouse brain tissue after dying of Venezuelan Encephalitis. Reveals neural necrosis and edema. ''Sourced from Wikimedia Commons, Copyright of the Centers for Disease Control and Prevention (CDC) Public Health Image Library (PHIL) Image #2809, 2006)'' ''']] | ||
− | + | ==Differential diagnoses<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>== | |
*Other togaviral encephalitides | *Other togaviral encephalitides | ||
+ | *Other [[Equine Viral Encephalitis|viral encephalitides]] | ||
*Trauma | *Trauma | ||
− | *Hepatic encephalopathy | + | *[[Equine Herpesvirus 1|Equine herpesvirus 1]] infection |
− | *Rabies | + | *[[Hepatic Encephalopathy|Hepatic encephalopathy]] |
+ | *[[Rabies]] | ||
+ | *Botulism | ||
*Leukoencephalomalacia | *Leukoencephalomalacia | ||
*Bacterial meningoencephalitis | *Bacterial meningoencephalitis | ||
− | *Equine protozoal myeloencephalitis (EPM) | + | *[[Equine Protozoal Myeloencephalitis|Equine protozoal myeloencephalitis]] (EPM) |
*Verminous encephalomyelitis | *Verminous encephalomyelitis | ||
− | *West Nile Virus (WNV) infection | + | *[[West Nile Virus|West Nile Virus (WNV)]] infection |
*Toxicosis | *Toxicosis | ||
− | + | ==Treatment<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>== | |
− | No effective, specific treatment is available. Supportive management includes: | + | No effective, specific treatment is available.<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> Supportive management includes: |
*NSAIDs (phenylbutazone, flunixin meglumine) to control pyrexia, inflammation and discomfort | *NSAIDs (phenylbutazone, flunixin meglumine) to control pyrexia, inflammation and discomfort | ||
*DMSO IV in a 20% solution to control inflamation, provide some analgesia and mild sedation | *DMSO IV in a 20% solution to control inflamation, provide some analgesia and mild sedation | ||
− | *Pentobarbital, diazepam IV, phenobarbital PO or phenytoin IV to control | + | *Pentobarbital, diazepam IV, phenobarbital PO or phenytoin IV to control seizures |
*Antibiotic therapy in cases with secondary bacterial infection | *Antibiotic therapy in cases with secondary bacterial infection | ||
*Balanced fluid solutions IV or PO as necessary to correct hydration status | *Balanced fluid solutions IV or PO as necessary to correct hydration status | ||
− | *Dietary supplementation (enteral or parenteral if anorexia persists more than 48 hours) | + | *Dietary supplementation (enteral, or parenteral if anorexia persists more than 48 hours) |
− | *Laxatives to | + | *Laxatives to reduce the risk of impaction |
− | *Protection of | + | *Protection of body regions susceptible to self-induced trauma and provision of deep bedding |
− | *Sling support | + | *Sling support for recumbent horses |
− | + | ==Prognosis== | |
− | Comatose animals rarely survive. Survivors exhibit functional improvement over weeks to months, but complete recovery from neurological deficits is rare. Residual ataxia, depression and abnormal behaviour is often seen with EEE, less | + | Comatose animals rarely survive. Survivors exhibit functional improvement over weeks to months, but complete recovery from neurological deficits is rare<ref>Patterson, J.S, Maes, R.K, Mullaney, T.P, Benson, C.L (1996) Immunohistochemical diagnosis of eastern equine encephalomyelitis, ''J Vet Diagn Invest'', 8(2):156-160. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Residual ataxia, depression and abnormal behaviour is often seen with EEE, but less so with WEE. The mortality rates for neurological equine viral encephalitis are reportedly: |
*EEE 75-100% | *EEE 75-100% | ||
*WEE 20-50% | *WEE 20-50% | ||
*VEE 40-80% | *VEE 40-80% | ||
− | It is generally assumed that infection does not provide protective immunity, however, protection for up to 2 years has been noted. | + | It is generally assumed that infection does not provide protective immunity, however, protection for up to 2 years has been noted.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> |
− | + | ||
− | + | ==Control== | |
− | + | === Vaccination=== | |
+ | Most vaccines are killed (produced in cell culture and inactivated with formalin)<ref name="manual">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> and elicit significant increases in Ab titre after 3 days.<ref name="attenuated">Ferguson, J.A, Reeves, W.C, Milby, M.M, Hardy, J.L (1978) Study of homologous and heterologous antibody responses in California horses vaccinated with attenuated Venezuelan equine encephalomyelitis vaccine (strain TC-83), ''Am J Vet Res'', 39:371-376. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Protective titres last for 6-8 months.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Some cross-protection is seen between the serotypes but not between Western and Eastern EEV.<ref>Jochim, M.M, Barber, T.L (1974) Immune response of horses after simultaneous or sequential vaccination against eastern, Western and Venezuelan equine encephlaomyelitis, ''J Am Vet Med Assoc'', 165:621-625. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Monovalent, divalent and trivalent vaccines are available but the response to monovalent VEE vaccination is decreased in horses previously vaccinated against WEE and EEE.<ref>Calisher, C.H, Sasso, D.R, Sather, G.E (1973) Possible evidence for interference with Venezuelan equine encephalitis virus vaccination of equines by pre-existing antibody to eastern or western equine encephalitis virus, or both, ''Appl Microbiol'', 26:485-488. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> The current recommendation is to vaccinate susceptible horses annually in late spring or several months before the high risk season.<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> Biannual or triannual vaccination should be employed in regions where the vector season is prolonged.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Susceptible horses should also be vaccinated in the face of an outbreak.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Mares should be vaccinated one month prior to foaling to boost colostral-derived Ab<ref name="again">Merck & Co (2008) The Merck Veterinary Manual (Eighth Edition), Merial found at http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/100900.htm&word=Equine%2cencephalitis, accessed July 2010</ref>, which persists for 6-7 months.<ref name="repeat"> Ferguson, J.A, Reeves, W.C, Hardy, J.L (1979) Studies on immunity to alphaviruses in foals, ''Am J Vet Res'', 40:5-10. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Although foals can be vaccinated at any time, early vaccination should be followed by boosters at 6 months and at one year. Vaccination does not interfere with the ELISA assay for VEE.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> '''NOTE''': Formalin-treated preparations of virulent epizootic VEE virus should never be used in equids as residual virulent virus can result in severe illness. Instead, the attenuated vaccine, strain TC-83<ref name="attenuated">Ferguson, J.A, Reeves, W.C, Milby, M.M, Hardy, J.L (1978) Study of homologous and heterologous antibody responses in California horses vaccinated with attenuated Venezuelan equine encephalomyelitis vaccine (strain TC-83), ''Am J Vet Res'', 39:371-376. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>, or an inactivated preparation created from that strain should be used. <ref>Walton, T.E, Grayson, M.A (1989)Chapter 46, Venezuelan equine encephalomyelitis. In: ''The Arboviruses: Epidemiology and Ecology'', Vol. 4, Monath T.P., ed. CRC Press, Boca Raton, Florida, USA, 203-231. In:''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref> '''''PRECAUTION'': human vaccination is recommended for vets in endemic areas'''. | ||
+ | |||
+ | ===Vector control=== | ||
+ | Responsible use of insecticides and repellents<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref>, elimination of standing water, and stable screening will all help to reduce viral transmission. Environmental application of insecticides may be useful in endemic areas or during an outbreak.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Horses infected with Venezuelan EEV should be isolated for 3 weeks after complete recovery.<ref name="same">Pasquini, C, Pasquini S, Woods, P (2005) '''Guide to Equine Clinics Volume 1: Equine Medicine''', third edition, p266, SUDZ publishing.</ref> | ||
+ | |||
+ | |||
+ | {{Learning | ||
+ | |literature search = [http://www.cabdirect.org/search.html?q=%28%28title%3A%28%22EEE%22%29%29%29+OR+%28%28title%3A%28%22Eastern+equine+encephalomyelitis%22%29%29%29+OR+%28%28title%3A%28%22Eastern+equine+encephalitis%22%29%29%29 Eastern Equine Encephalomyelitis publications] | ||
+ | |||
+ | [http://www.cabdirect.org/search.html?q=%28%28title%3A%28%22WEE%22%29%29%29+OR+%28%28title%3A%28%22Western+equine+encephalomyelitis%22%29%29%29+OR+%28%28title%3A%28%22Western+equine+encephalitis%22%29%29%29 Western Equine Encephalomyelitis publications] | ||
+ | |||
+ | [http://www.cabdirect.org/search.html?q=%28%28title%3A%28%22VEE%22%29%29%29+OR+%28%28title%3A%28%22Venezuelan+equine+encephalomyelitis%22%29%29%29+OR+%28%28title%3A%28%22Venezuelan+equine+encephalitis%22%29%29%29 Venezuelan Equine Encephalomyelitis publications] | ||
− | + | [http://www.cabdirect.org/search.html?q=%28%28title%3A%28treatment%29%29%29+AND+%28%28%28%28%28%28title%3A%28%22EEE%22%29%29%29+OR+%28%28title%3A%28%22Eastern+equine+encephalomyelitis%22%29%29%29+OR+%28%28title%3A%28%22Eastern+equine+encephalitis%22%29%29%29%29%29+OR+%28%28%28%28title%3A%28%22WEE%22%29%29%29+OR+%28%28title%3A%28%22Western+equine+encephalomyelitis%22%29%29%29+OR+%28%28title%3A%28%22Western+equine+encephalitis%22%29%29%29%29%29+OR+%28%28%28%28title%3A%28%22VEE%22%29%29%29+OR+%28%28title%3A%28%22Venezuelan+equine+encephalomyelitis%22%29%29%29+OR+%28%28title%3A%28%22Venezuelan+equine+encephalitis%22%29%29%29%29%29%29%29 Equine Togaviral Encephalomyelitis Treatment publications] | |
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− | + | ==References== | |
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Latest revision as of 15:24, 6 July 2012
Also known as: Alphaviral encephalitis — Alphaviral encephalitides — Eastern equine encephalitis — Eastern equine encephalomyelitis — EEE — Western equine encephalitis — Western equine encephalomyelitis — WEE — Venezuelan equine encephalitis — Venezuelan equine encephalomyelitis — VEE
Introduction
NOTIFIABLE and ZOONOTIC OIE List B, infectious, mosquito-borne diseases of equidae affecting the central nervous system (CNS). They include:
- Eastern Equine Encephalitis (EEE)
- Western Equine Encephalitis (WEE)
- Venezuelan Equine Encephalitis (VEE) - reportable in the USA[1]
Aetiology
See this page for details of the equine encephalitis causal pathogens. Some of the virus strains can infect swine[2], poultry and other farmed birds including quail and ratites.[3] Isolated cases have also been noted in cattle[4], sheep and non-domestic ungulates.[3] Some strains are potential agents of biowarfare or bioterrorism[5].
Epidemiology
Distribution
Togaviral encephalitis in equids is largely confined to the Western Hemisphere.[3] Venezuelan EEV can cause large outbreaks of disease over extensive geographical areas in both humans and horses. Spread of this virus into Central America has had disasterous consequences with epidemics as far north as Texas.[6] Climatic conditions and interventions that support vector populations, such as irrigation, greatly influence the geographical spread of the disease.[6] EEE has been recorded across the United States, but mostly in the Southeastern States.[3] As its names suggests, WEE has a predilection for the Western states which have been subject to significant outbreaks in the past. A regional alteration in virulence has been proposed for the steep decline in clinical case numbers observed in this area.[3] A lag phase of 2-5weeks is commonly observed between horse and human cases of WEE in a given locus.[7] Both are dead-end hosts for the virus. A subtype of Western EEV, Highlands J virus, was isolated from the brain of a horse with encephalitis in Florida.[8]
Transmission
Transfer is vector-mediated, primarily via mosquito salivary transfer. WEE and VEE may also be transmitted horse to horse through nasal secretions. This mode of transmission is less likely, despite the fact that high concentrations of VEE virus are found in ocular and nasal discharges from infected horses.[9] The viraemic phase ends when nervous signs develop[3] and is important for disease amplification. Amplification from horses is likely only with VEE virus, in association with a relatively high and potentially persistent viraemia.[10] Similarly, zoonotic spread is unlikely for Eastern and Western equine encephalitis, but has been noted with VEE.
Seasonal Incidence
The disease is not directly contagious between horses and humans but occurs sporadically in both species from mid-summer to late autumn - during the height of the vector season.[1] Case numbers peak in June to November in temperate climates.[3] The vector season is longer in warmer climates, where the disease period is prolonged. Global warming may promote more outbreaks in historically colder climates.[3]
Epidemics
Outbeak prediction to date has been inaccurate, implying that other, unidentified factors may be in operation.[3] However, some epidemic requirements are beyond question. Adequate amounts of infective virus, sufficent vectors, infected sylvatic hosts and susceptible terminal hosts, and finally, appropriate reservoirs, are all crucial.[11]
Pathogenesis[3]
Upon entry to the host, viruses multiply in the muscle, enter the lymphatic circulation and localize in lymph nodes. In macrophages and neutrophils viral replication leads to shedding and significant clearance of viral particles. No further clinical signs develop if this clearance is successful. Erythrocyte and leukocyte absorption are used to circumvent the immune defences of the host. After incomplete elimination, residual virus infects endothelial cells and accumulates in highly vascular organs such as the liver and spleen. In these organs, viral replication produces circulating virus and a second viraemic period, typically associated with early clinical signs. Neuroinvasion and replication occurs within a week.[10] An incubation period of 7-21days has been demonstrated after experimental infection with Eastern or Western EEV, but the incubation is often shorter for EEE compared with that of WEE.
Signalment
Unvaccinated adult horses and other equids (VEE in donkeys) are at risk in areas with suitable vectors. Vaccinated horses can still develop the disease, particularly if they are young or old.
Clinical Signs
Worse in unvaccinated horses.[3] Neurological signs may be assymmetrical.[10]
EEE and WEE
Following an incubation period of up to 21days[3], an initial pyrexia and mild depression are short-lived and often missed. The acute phase of the disease presents with mild to severe pyrexia, anorexia and stiffness, lasting up to 5 days. During this time, the horse is viraemic and capable of amplifying the disease. The fever may then fluctuate with neurological derangements appearing a few days post-infection.[1] These changes indicate disease progression, which occurs more frequently with EEE (the most virulent of the three serotypes). Any of the following may be observed[3]:
- conscious proprioceptive deficits
- propulsive walking
- depression
- somnolence
- aggression
- excitability
- restlessness
- hypersensitivity to sound and touch
Worsening neurological deficits may result in[3]:
- head pressing
- head tilt
- ataxia
- circling
- apparent blindness
- facial and appendicular muscle fasciculations
- pendulous lower lip[1]
- pharynx, larynx and tongue paralysis
- seizures[1]
- recumbency for 1-7 days followed by death[1]
VEE
Clinical signs may resemble those of WEE and EEE or may vary[3]:
- pyrexia peaks early and persists throughout the disease course
- mild pyrexia and leukopenia with endemic strains
- severe pyrexia and leukopenia with epidemic strains
- neurological signs around 4 days post-infection
- diarrhoea, severe depression, recumbency and death may precede neurological signs
- other signs: abortion, oral ulceration, pulmonary haemorrhage, epistaxis
Diagnosis
Presumptive based on history, epidemiology and clinical signs.[1] Definitive diagnosis requires virus identification, serological tests and/or post-mortem examination.
Laboratory Tests
Virus Identification
Serology
Ab titre increases sharply within 24 hours of the initial viraemia, before clinical signs are apparent. It then deteriorates over 6 months. Samples taken when clinical signs appear are likely to miss the Ab peak and will demonstrate a decreasing titre.[3] Thus, serological confirmation of Eastern or Western EEV infection requires a four-fold or greater increase[1] OR decrease in Ab titre in paired serum samples taken 10-14 days apart.[12] A presumptive diagnosis can be made on a single sample if an unvaccinated horse with suggestive clinical signs has Ab against only Eastern or Western EEV.[12] Colostral-derived Ab has a serum half-life of around 20days and may interfere with diagnosis in foals.[13]
- Complement fixation (CF): to avoid anti-complementary effects, serum should be separated from blood as soon as possible.[12] CF Ab against both Eastern and Western EEV is less useful for serological diagnosis because it appears relatively late and does not persist.[12]
- Haemagglutination inhibition (HAI): titres of 1/10 and 1/20 are indicative, titres of 1/40 and above are positive.[12]
- ELISA may be used to detect viral-specific IgM to the surface glycoprotein of Venezuelan EEV, from 3 days post-onset of clinical signs up to 21 days post-infection. This is useful in acute infections where convalescent serum samples are unobtainable.[3]
- The plaque reduction neutralization (PRN) test is very specific and can differentiate EEE and WEE infections. It is performed in duck embryo fibroblast, Vero, or BHK-21 cell cultures. Serum is tested against 100 plaque-forming units of virus. Endpoints are based on a 90% reduction in the number of plaques compared with the virus control.[12]
Most commonly, the PRN test or a combination of PRN and HAI tests is used to detect Ab against Eastern and Western EEV. Cross-reactivity occurs between Ab against these viruses in the CF and HAI tests.[12]
Clinical Pathology
CSF samples demonstrate increased cellularity (50-400 mononuclear cells/µl) and protein concentration (100-200mg/dl).[3]
Post-mortem findings
PRECAUTION: infective viral particles may be present in CNS and other tissues.[3] Gross pathological lesions of the brain and spinal cord are rarely seen in horses, although traumatic ecchymotic haemorrhages and vascular congestion of the CNS may be evident.[12] The extent of microscopic lesions is dictated by the severity of infection and duration of neurological involvement.[14] Such lesions with or without immunohistochemistry may be diagnostic.[12] The cerebral cortex, thalamus and hypothalamus are often severely affected. Mononuclear meningitis, neuronal degeneration, diffuse and focal gliosis and perivascular cuffing are also seen.[12] Histological lesions of EEE are usually present throughout the CNS, with widespread and severe neutrophilic inflammation of the grey matter. Lesions caused by Western EEV infection are more focal and lymphocytic in nature.[12] VEE cases often exhibit haemorrhage and liquefactive necrosis of the cerebral cortex, but lesions are not restricted to the CNS. In the pancreas, acinar cells atrophy and duct cells undergo hyperplasia.[15] There may also be damage to the liver and heart.[3]
Differential diagnoses[3]
- Other togaviral encephalitides
- Other viral encephalitides
- Trauma
- Equine herpesvirus 1 infection
- Hepatic encephalopathy
- Rabies
- Botulism
- Leukoencephalomalacia
- Bacterial meningoencephalitis
- Equine protozoal myeloencephalitis (EPM)
- Verminous encephalomyelitis
- West Nile Virus (WNV) infection
- Toxicosis
Treatment[3]
No effective, specific treatment is available.[1] Supportive management includes:
- NSAIDs (phenylbutazone, flunixin meglumine) to control pyrexia, inflammation and discomfort
- DMSO IV in a 20% solution to control inflamation, provide some analgesia and mild sedation
- Pentobarbital, diazepam IV, phenobarbital PO or phenytoin IV to control seizures
- Antibiotic therapy in cases with secondary bacterial infection
- Balanced fluid solutions IV or PO as necessary to correct hydration status
- Dietary supplementation (enteral, or parenteral if anorexia persists more than 48 hours)
- Laxatives to reduce the risk of impaction
- Protection of body regions susceptible to self-induced trauma and provision of deep bedding
- Sling support for recumbent horses
Prognosis
Comatose animals rarely survive. Survivors exhibit functional improvement over weeks to months, but complete recovery from neurological deficits is rare[16] Residual ataxia, depression and abnormal behaviour is often seen with EEE, but less so with WEE. The mortality rates for neurological equine viral encephalitis are reportedly:
- EEE 75-100%
- WEE 20-50%
- VEE 40-80%
It is generally assumed that infection does not provide protective immunity, however, protection for up to 2 years has been noted.[3]
Control
Vaccination
Most vaccines are killed (produced in cell culture and inactivated with formalin)[12] and elicit significant increases in Ab titre after 3 days.[17] Protective titres last for 6-8 months.[3] Some cross-protection is seen between the serotypes but not between Western and Eastern EEV.[18] Monovalent, divalent and trivalent vaccines are available but the response to monovalent VEE vaccination is decreased in horses previously vaccinated against WEE and EEE.[19] The current recommendation is to vaccinate susceptible horses annually in late spring or several months before the high risk season.[1] Biannual or triannual vaccination should be employed in regions where the vector season is prolonged.[3] Susceptible horses should also be vaccinated in the face of an outbreak.[3] Mares should be vaccinated one month prior to foaling to boost colostral-derived Ab[10], which persists for 6-7 months.[13] Although foals can be vaccinated at any time, early vaccination should be followed by boosters at 6 months and at one year. Vaccination does not interfere with the ELISA assay for VEE.[3] NOTE: Formalin-treated preparations of virulent epizootic VEE virus should never be used in equids as residual virulent virus can result in severe illness. Instead, the attenuated vaccine, strain TC-83[17], or an inactivated preparation created from that strain should be used. [20] PRECAUTION: human vaccination is recommended for vets in endemic areas.
Vector control
Responsible use of insecticides and repellents[1], elimination of standing water, and stable screening will all help to reduce viral transmission. Environmental application of insecticides may be useful in endemic areas or during an outbreak.[3] Horses infected with Venezuelan EEV should be isolated for 3 weeks after complete recovery.[1]
Equine Togaviral Encephalitis Learning Resources | |
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Eastern Equine Encephalomyelitis publications
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References
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Pasquini, C, Pasquini S, Woods, P (2005) Guide to Equine Clinics Volume 1: Equine Medicine, third edition, p266, SUDZ publishing.
- ↑ Karsted, L, Hansen, R.P (1959) Natural and experimental infections in swine with the virus of eastern equine encephalomyelitis, J Infect Dis 105:293-296. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 3.24 3.25 3.26 3.27 Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ Pursell, A.R, Mitchell, F.E, Seibold, H.R (1976) Naturally occurring and experimentally induced eastern encephalomyelitis in calves, J Am Vet Med Assoc, 169:1101-1103. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ Steele, K.E, Twenhafel, N.A (2010) Review Paper: Pathology of Animal Models of Alphavirus Encephalitis. Vet Pathol. Jun 15. [Epub ahead of print].
- ↑ 6.0 6.1 http://www.defra.gov.uk/foodfarm/farmanimal/diseases/atoz/viralenceph/index.htm, accessed July 2010
- ↑ McLintock, J (1980) The arbovirus problem in Canada, Can J Public Health, 67(Suppl 1):8-12. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ Karabatsos, N, Lewis, A.L, Calisher, C.H, Hunt, A.R, and Roehrig, J.T (1988). Identification of Highland J virus from a Florida horse. Am J Trop Med Hyg, 39, 603-606. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.
- ↑ Kissling, R.E, Chamberlain, R.W (1967) Venezuelan equine encephalitis, Adv Vet Sci Comp Med, 11:65-84. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ 10.0 10.1 10.2 10.3 Merck & Co (2008) The Merck Veterinary Manual (Eighth Edition), Merial found at http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/100900.htm&word=Equine%2cencephalitis, accessed July 2010
- ↑ Sellers, R.F (1980) Weather, host and vector: their interplay in the spread of insect-borne animal virus diseases, J Hyg (Lond), 85:65-102. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ 12.00 12.01 12.02 12.03 12.04 12.05 12.06 12.07 12.08 12.09 12.10 12.11 Manual of Diagnostic Tests and Vaccines for Terrestrial Animals found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.
- ↑ 13.0 13.1 Ferguson, J.A, Reeves, W.C, Hardy, J.L (1979) Studies on immunity to alphaviruses in foals, Am J Vet Res, 40:5-10. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ Walton T.E (1981) Venezuelan, eastern, and western encephalomyelitis. In: Virus Diseases of Food Animals. A World Geography of Epidemiology and Control. Disease Monographs, Vol. 2, Gibbs E.P.J, ed. Academic Press, New York, USA, 587-625.
- ↑ Monlux, W.S, Luedke, A.J (1973) Brain and spinal cord lesions in horses inoculated with Venezuelan equine encephalomyelitis virus (epidemic American and Trinidad strains), Am J Vet Res, 34:465-473. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ Patterson, J.S, Maes, R.K, Mullaney, T.P, Benson, C.L (1996) Immunohistochemical diagnosis of eastern equine encephalomyelitis, J Vet Diagn Invest, 8(2):156-160. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ 17.0 17.1 Ferguson, J.A, Reeves, W.C, Milby, M.M, Hardy, J.L (1978) Study of homologous and heterologous antibody responses in California horses vaccinated with attenuated Venezuelan equine encephalomyelitis vaccine (strain TC-83), Am J Vet Res, 39:371-376. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ Jochim, M.M, Barber, T.L (1974) Immune response of horses after simultaneous or sequential vaccination against eastern, Western and Venezuelan equine encephlaomyelitis, J Am Vet Med Assoc, 165:621-625. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ Calisher, C.H, Sasso, D.R, Sather, G.E (1973) Possible evidence for interference with Venezuelan equine encephalitis virus vaccination of equines by pre-existing antibody to eastern or western equine encephalitis virus, or both, Appl Microbiol, 26:485-488. In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) Equine Internal Medicine (Third Edition), Saunders, Chapter 12
- ↑ Walton, T.E, Grayson, M.A (1989)Chapter 46, Venezuelan equine encephalomyelitis. In: The Arboviruses: Epidemiology and Ecology, Vol. 4, Monath T.P., ed. CRC Press, Boca Raton, Florida, USA, 203-231. In:Manual of Diagnostic Tests and Vaccines for Terrestrial Animals found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.
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