Difference between revisions of "Giardiasis"
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===Laboratory Tests=== | ===Laboratory Tests=== | ||
| − | + | [[Image:Giardiasis duodenum high power.jpg|thumb|right|150px|Giardia trophozoites in the duodenum. Source: Wikimedia Commons; Author: Nephron (2009)]] | |
Diagnosis is confirmed by demonstrating ''[[Giardia]]'' antigen, trophozoites or cysts in the faeces. The simplest method is to examine fresh faecal smears for cysts (and occasional trophozoites), but sensitivity is improved by using concentration techniques. Centrifugal flotation in zinc sulphate solution or sugar flotation solution are both appropriate, but zinc sulphate solution is preferred as it causes minimal distoryion to cysts. In this technique, faecal material is centrifuged and saturated with zinc sulphate mixture before the fluid from the top of the tube is placed on a microscope slide. Diff-Quik or Lugol's iodine are used to stain cysts and trophozoites, a coverslip is applied, and the slide is examined under the microscope. Although infrequently detected, ''[[Giardia]]'' trophozoites are approximately 8 x 15 micron tear-drop shaped organisms which contain two nuclei. They can sometimes be seen to move in a "falling leaf" pattern. Cysts are around 12 microns long and, if not distorted, oval in shape. When looking for cysts, three samples collected at two to three day intervals should be examined as shedding is intermittent. | Diagnosis is confirmed by demonstrating ''[[Giardia]]'' antigen, trophozoites or cysts in the faeces. The simplest method is to examine fresh faecal smears for cysts (and occasional trophozoites), but sensitivity is improved by using concentration techniques. Centrifugal flotation in zinc sulphate solution or sugar flotation solution are both appropriate, but zinc sulphate solution is preferred as it causes minimal distoryion to cysts. In this technique, faecal material is centrifuged and saturated with zinc sulphate mixture before the fluid from the top of the tube is placed on a microscope slide. Diff-Quik or Lugol's iodine are used to stain cysts and trophozoites, a coverslip is applied, and the slide is examined under the microscope. Although infrequently detected, ''[[Giardia]]'' trophozoites are approximately 8 x 15 micron tear-drop shaped organisms which contain two nuclei. They can sometimes be seen to move in a "falling leaf" pattern. Cysts are around 12 microns long and, if not distorted, oval in shape. When looking for cysts, three samples collected at two to three day intervals should be examined as shedding is intermittent. | ||
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===Endoscopy=== | ===Endoscopy=== | ||
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The above tests may also be performed using duodenal aspirates obtained by endoscopy. | The above tests may also be performed using duodenal aspirates obtained by endoscopy. | ||
===Pathology=== | ===Pathology=== | ||
| − | Gross lesions are not normally found at post-mortem examination. | + | Gross lesions are not normally found at post-mortem examination. Giardia trophozoites may be seen in the lumen following intestinal biopsy. |
==Treatment== | ==Treatment== | ||
Revision as of 18:37, 11 August 2010
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This article is still under construction. |
| Also known as: | Giardiosis Giardosis Giardia Infection Lambliasis Lambliosis |
Description
Giardia is a binucleate, flagellate protozoa that causes enteric infection giardiasis in dogs and occasionally cats. Giardia species have been reported to be found in 1-39% of faecal samples from dogs and cats, with a higher rate of infection in younger animals.Transmission is via cysts that are shed in the faeces, and may be direct or indirect. Indirect water-borne transmission is the most common modality and is favoured by cool, moist conditions that prolong cyst survival. Once cysts are ingested, motile trophozoites are released and attach to the cells of the small intestinal mucosa via an adhesive disc. Predilection sites in the dog are the duodenum and jejunum, where as only the duodenum is favoured in cats. Multiplication by binary fission can then occur, and trophozoites encyst to a stage adapted for survival in the external environment. Cysts are seen in the faeces from day 3 post-infection in dogs (5-16 in cats), and can survive in the environment for several months. Animals that mount an immune response to Giardia can eliminate infection; however, the immunity acquired does not necessarily prevent re-infection after a short period.
Giardiasis is a condition of significant zoonotic concern. Although the cat protozoa G. felis seems to be species-specific, dogs can host both the canine-specific G. canis and G. lamblia, which has zoonotic genotypes. It is not known how important dogs are as a reservoir for human Giardia infection, or how frequently zoonotic transmission occurs. In man, Giardia may cause acute diarrhoea, particualrly in immunocompromised individuals. Subclinical infections also occur.
Signalment
Dogs are more commonly affected than cats. There are no age, breed or sex predispositions for Giardia infection, but puppies and kittens are more likely to contract clinical disease than adult animals.
Diagnosis
Clinical Signs
Clinical signs are most likely to be seen in younger animals as older hosts are usually infected asymptomatically. Signs vary from acute, chronic or intermittent diarrhoea to chronic loose, fould-smelling faeces, and frequency of defaecation is usually increased. The stools are normally light coloured and mucoid and often contain fat. Blood is not normally present in the faeces. Vomiting, flatulence and weight loss/failure to gain weight may be seen in some cases, and the hair coat may be poor. Giardiasis may also cause malabsorption, and there are several proposed mechanisms for this. For example, the small intestinal villi may be grossly atrophies or damages ultrastructurally, or giardiasis may lead to bacterial overgrowth. There is also evidence that Giardia consumes bile acids, preventing digestion of fats.
Laboratory Tests
Diagnosis is confirmed by demonstrating Giardia antigen, trophozoites or cysts in the faeces. The simplest method is to examine fresh faecal smears for cysts (and occasional trophozoites), but sensitivity is improved by using concentration techniques. Centrifugal flotation in zinc sulphate solution or sugar flotation solution are both appropriate, but zinc sulphate solution is preferred as it causes minimal distoryion to cysts. In this technique, faecal material is centrifuged and saturated with zinc sulphate mixture before the fluid from the top of the tube is placed on a microscope slide. Diff-Quik or Lugol's iodine are used to stain cysts and trophozoites, a coverslip is applied, and the slide is examined under the microscope. Although infrequently detected, Giardia trophozoites are approximately 8 x 15 micron tear-drop shaped organisms which contain two nuclei. They can sometimes be seen to move in a "falling leaf" pattern. Cysts are around 12 microns long and, if not distorted, oval in shape. When looking for cysts, three samples collected at two to three day intervals should be examined as shedding is intermittent.
Commercial ELISA tests are now available for the detection of Giardia antigen in faeces. These include the Snap Giardia test from IDEXX and the ProSpectT/Giardia ELISA from Alexon-Trend.
Endoscopy
The above tests may also be performed using duodenal aspirates obtained by endoscopy.
Pathology
Gross lesions are not normally found at post-mortem examination. Giardia trophozoites may be seen in the lumen following intestinal biopsy.
Treatment
It is particularly important to treat giardiasis in animals owing to its zoonotic potential. In the UK, fenbendazole is licensed for use against Giardia at a dose rate of 50 mg/kg daily for three days. Metronidazole may also be used at 20mg/kg for five days in dogs, and at 25mg/kg for seven days in cats. However, metronidazole is only 67% effective in dogs and has a bitter taste. A combination of febantel, praziquantel and febantel (Drontal Plus) can be used in dogs. Following treatment, it is advisable to repeat faecal examinations to confirm the efficacy of treatment and to detect re-infection. As well as drug therapy, thorough cleaning and disinfection of the enivronment and the animal's belongings is essential. Shedding of cysts can be difficult to detect and so in contact animals may be treated concurrently.
A vaccine comprising killed trophozoites has been developed and is available in the USA for use in dogs and cats. The vaccine decreases the excretion and viability of Giardia cysts, thus reducing the environmental burden.
Prognosis
Treatment is usually effective and the prognosis for treated giardiasis in dogs and cats is good. However, chronic infection may lead to debilitation.
Links
- Giardiasis factsheet from The Center for Food Security and Public Health, Iowa State University
- The Merck Veterinary Manual - Giardiasis
- The Merck Online Medical Library - Giardiasis in humans
- Government of Alberta, Agriculture and Rural Development - Giardia in Dogs
References
- Fisher, M (2002) Endoparasites in the dog and cat: 2. Protozoa. In Practice, 24(3), 146-153.
- Taylor, M A and Webster, K A (1998) Recent advances in the diagnosis of Cryptosporidium, Toxoplasma, Giardia and other protozoa of veterinary importance. Research in Veterinary Science, 65, 183-193.
- Papasouliotis, K and Gruffydd-Jones, T (1996) Practical Approach to Diarrhoes in the Cat. In Practice, 18(5), 206-214.
- Tilley, L P and Smith, F W K (2007) The 5-minute Veterinary Consult (Fourth Edition) Blackwell Publishing.
- Rutgers, H C (1989) Diarrhoea in the Cat. In Practice, 11(4), 139-148.
- Merck & Co (2008) The Merck Veterinary Manual (Eighth Edition) Merial