Difference between revisions of "Immunological Testing Flashcards"

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==<font color="purple">Radioimmunoassay</font>==
 
==<font color="purple">Radioimmunoassay</font>==
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{| border="3" cellpadding="8"
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<FlashCard questions="4">
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!width="400"|'''Question'''
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|q1=What are radioimmunoassays commonly used to detect?
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!width="400"|'''Answer'''
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|a2=
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!width="150"|'''Article'''
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*Hormone levels in blood and tissue fluids
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|-
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*Serum proteins
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|<big>'''What are radioimmunoassays commonly used to detect?'''
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*Drugs
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||<font color="white"> <big>
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*Vitamins
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*'''''Hormone levels in blood and tissue fluids'''''
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|l1=Radioimmunoassay
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*'''''Serum proteins'''''
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|q2=What is the most common isotope used for radioimmunoassay?
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*'''''Drugs'''''
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|a2=Iodine-125
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*'''''Vitamins'''''
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|l2=Radioimmunoassay
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||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
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|q3=Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?
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|-
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|a3=Ensures the number of epitopes present exceeds number of antibody-binding sites
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|<big>'''What is the most common isotope used for radioimmunoassay?'''
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|l3=Radioimmunoassay
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||<font color="white"> <big>
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|q4=What are the disadvantages of the radioimmunoassay?
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*'''''Iodine-125'''''
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|a4=
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||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
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*Substances used are radioactive
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|-
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*Gamma radiation requires special counting equipment
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|<big>'''Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?'''
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*Iodine naturally accumulates in the thyroid gland
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||<font color="white"> <big>
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|l4=Radioimmunoassay
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*'''''Ensures the number of epitopes present exceeds number of antibody-binding sites'''''
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</FlashCard>
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||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
 
−
|-
 
−
|<big>'''What are the disadvantages of the radioimmunoassay?'''
 
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||<font color="white"> <big>
 
−
*'''''Substances used are radioactive'''''
 
−
*'''''Gamma radiation requires special counting equipment'''''
 
−
*'''''Iodine naturally accumulates in the thyroid gland'''''
 
−
||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]]
 
  
−
|}
 
 
==<font color="purple">Agglutination</font>==
 
==<font color="purple">Agglutination</font>==
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{| border="3" cellpadding="8"
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<FlashCard questions="5">
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!width="400"|'''Question'''
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|q1=What are the antibodies that produce agglutination known as?
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!width="400"|'''Answer'''
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|a1=Agglutinins
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!width="150"|'''Article'''
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|l1=Agglutination
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|-
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|q2=What constitutes the end of the agglutination test?
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|<big>'''What are the antibodies that produce agglutination known as?'''
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|a2=The last tube showing distinct agglutination
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||<font color="white"> <big>
+
|l2=Agglutination
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*'''''Agglutinins'''''
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|q3=What does the titre represent?
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||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
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|a3=The number of antibodies per unit volume of serum
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|-
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|l3=Agglutination
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|<big>'''What constitutes the end of the agglutination test?'''
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|q4=What is the prozone effect?
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||<font color="white"> <big>
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|a4=An inhibition of agglutination caused by an excess of antibodies
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*'''''The last tube showing distinct agglutination'''''
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|l4=Agglutination|
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||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
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|q5=What agglutination test is often used to test for rhesus disease?
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|-
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|a5=Coombs test
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|<big>'''What does the titre represent?'''
+
|l5=Agglutination
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||<font color="white"> <big>
+
</FlashCard>
−
*'''''The number of antibodies per unit volume of serum'''''
 
−
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 
−
|-
 
−
|<big>'''What is the prozone effect?'''
 
−
||<font color="white"> <big>
 
−
*'''''An inhibition of agglutination caused by an excess of antibodies'''''
 
−
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 
−
|-
 
−
|<big>'''What agglutination test is often used to test for rhesus disease?'''
 
−
||<font color="white"> <big>
 
−
*'''''Coombs test'''''
 
−
||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]]
 
  
  

Revision as of 17:10, 17 March 2012

ELISA testing

Question Answer Article
What does the acronym ELISA stand for? Link to Article
What type of ELISA requires all the reagents to be added at once? Link to Article
What are the five basic steps of an ELISA test? Link to Article


Immunofluorescence

Question Answer Article
The adsorption of light by a molecule is known as what? Link to Article
What are the fluorescent dyes commonly known as? Link to Article
What are the advantages of indirect staining? Link to Article
What technique is used to provide a quantitative measure of immunofluorescence? Link to Article


Radioimmunoassay

Question Answer Article
What are radioimmunoassays commonly used to detect? Link to Article
What is the most common isotope used for radioimmunoassay? Link to Article
Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen? Link to Article
What are the disadvantages of the radioimmunoassay? Link to Article


Agglutination

Question Answer Article
What are the antibodies that produce agglutination known as? Link to Article
What constitutes the end of the agglutination test? Link to Article
What does the titre represent? Link to Article
What is the prozone effect? Link to Article
What agglutination test is often used to test for rhesus disease? Link to Article





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