Difference between revisions of "Immunological Testing Flashcards"

Revision as of 10:59, 15 September 2008

IMMUNOLOGICAL TESTING WIKIBLOOD FLASHCARDS

Question	Answer	Article
What does the acronym ELISA stand for?	Enzyme Linked ImmunoSorbent Assay	Link to Answer Article
What type of ELISA requires all the reagents to be added at once?	Homogenous	Link to Answer Article
What are the five basic steps of an ELISA test?	Adsorption of antigen/antibody onto solid phase Addition of chosen sample and reagents Incubation and washing Addition of enzyme-labelled antigen/antibody Addition of specific substrate	Link to Answer Article

Question	Answer	Article
The adsorption of light by a molecule is known as what?	Excitation	Link to Answer Article
What are the fluorescent dyes commonly known as?	Fluorochromes	Link to Answer Article
What are the advantages of indirect staining?	Brighter fluorescence One preparation can be used to test many serum samples Relative expressions of different antigens in the same cell can be tested Loss of antibody during conjugation is limited	Link to Answer Article

@@ Line 31: / Line 31: @@
 *'''''Addition of enzyme-labelled antigen/antibody'''''
 *'''''Addition of specific substrate'''''
+||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
+|}
+==<font color="purple">Immunofluorescence</font>==
+{| border="3" cellpadding="8"
+!width="400"|'''Question'''
+!width="400"|'''Answer'''
+!width="150"|'''Article'''
+|-
+|<big>'''The adsorption of light by a molecule is known as what?'''
+||<font color="white"> <big>
+*'''''Excitation'''''
+||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
+|-
+|<big>'''What are the fluorescent dyes commonly known as?'''
+||<font color="white"> <big>
+*'''''Fluorochromes'''''
+||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]
+|-
+|<big>'''What are the advantages of indirect staining?'''
+||<font color="white"> <big>
+*'''''Brighter fluorescence'''''
+*'''''One preparation can be used to test many serum samples'''''
+*'''''Relative expressions of different antigens in the same cell can be tested'''''
+*'''''Loss of antibody during conjugation is limited'''''
 ||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]]