Giardiasis
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This article is still under construction. |
| Also known as: | Giardiosis Giardia Infection |
Description
Giardia is a binucleate, flagellate protozoa that causes enteric infection giardiasis in dogs and occasionally cats. Transmission is via cysts that are shed in the faeces, and may be direct or indirect. Indirect water-borne transmission is the most common modality and is favoured by cool, moist conditions that prolong cyst survival. Once cysts are ingested, motile trophozoites are released and attach to the cells of the small intestinal mucosa via an adhesive disc. Predilection sites in the dog are the duodenum and jejunum, where as only the duodenum is favoured in cats. Multiplication by binary fission can then occur, and trophozoites encyst to a stage adapted for survival in the external environment. Cysts are seen in the faeces from day 3 post-infection in dogs (5-16 in cats), and can survive in the environment for several months. Animals that mount an immune response to Giardia can eliminate infection; however, the immunity acquired does not necessarily prevent re-infection after a short period.
Giardiasis is a condition of significant zoonotic concern. Although the cat protozoa G. felis seems to be species-specific, dogs can host both the canine-specific G. canis and G. lamblia, which has zoonotic genotypes. It is not known how important dogs are as a reservoir for human Giardia infection, or how frequently zoonotic transmission occurs. In man, Giardia may cause acute diarrhoea, particualrly in immunocompromised individuals. Subclinical infections also occur.
Signalment
Dogs are more commonly affected than cats. There are no age, breed or sex predispositions for Giardia infection, but puppies and kittens are more likely to contract clinical disease than adult animals.
Diagnosis
Clinical Signs
Clinical signs are most likely to be seen in younger animals as older hosts are usually infected asymptomatically. Signs vary from acute diarrhoea to chronic loose, fould-smelling faeces, and frequency of defaecation is usually increased. Vomiting and weight loss may be seen in some cases. Malabsorbtion syndrome with soft, frothy, greasy, voluminous, malodourous faeces can occur.
Giardiasis may also cause malabsorption, and there are several proposed mechanisms for this. For example, the small intestinal villi may be grossly atrophies or damages ultrastructurally, or giardiasis may lead to bacterial overgrowth. There is also evidence that Giardia consumes bile acids, preventing digestion of fats.
Laboratory Tests
Diagnosis is confirmed by demonstrating Giardia antigen, trophozoites or cysts in the faeces. The simplest method is to examine fresh faecal smears for cysts (and occasional trophozoites), but sensitivity is improved by using concentration techniques. Centrifugal flotation in zinc sulphate solution or sugar flotation solution are both appropriate, but zinc sulphate solution is preferred as it causes minimal distoryion to cysts. In this technique, faecal material is centrifuged and saturated with zinc sulphate mixture before the fluid from the top of the tube is placed on a microscope slide. Diff-Quik or Lugol's iodine are used to stain cysts and trophozoites, a coverslip is applied, and the slide is examined under the microscope. Although infrequently detected, Giardia trophozoites are approximately 8 x 15 micron tear-drop shaped organisms which contain two nuclei. They can sometimes be seen to move in a "falling leaf" pattern. Cysts are around 12 microns long and, if not distorted, oval in shape. When looking for cysts, three samples collected at two to three day intervals should be examined as shedding is intermittent.
Commercial ELISA tests are now available for the detection of Giardia antigen in faeces. These include the Snap Giardia test from IDEXX and the ProSpectT/Giardia ELISA from Alexon-Trend.
Endoscopy
The above tests may also be performed using duodenal aspirates obtained by endoscopy.
Treatment
Various treatments for Giardia infection have been reported in the literature. In the 1990s, interest increased in the use of the benzimidazoles and now fenbendazole (Panacur; Intervet) is licensed for the treatment of Giardia infection at a dose rate of 50 mg/kg daily for three days. Giardia infection can be difficult to control, particularly in the kennel situation where infection can circulate between dogs and environmental contamination can be substantial. A thorough cleaning programme to remove debris, followed by disinfection with, for example, a quaternary ammonium-containing compound, is essential. It may also be advisable to treat all affected dogs and in-contact animals at the same time, given that shedding of cysts can be hard to detect.
- Sanitation and hygiene
- Minimise risk of human infection
- Boil (or sterilise through other means) drinking water in regions of the world where drinking water may be contaminated
- Avoid uncooked foodstuffs
- Contaminated by washing
- Drug Treatments
- Metronidazole
- Flagyl, Torgyl
- Fenbendazole
- Panacur
- Metronidazole
- Vaccination
- Killed trophozoites
- Available in the USA for cats and dogs
- Decreases the excretion and viability of cysts
- Decreases environmental pressure
Prognosis
Links
- The Merck Veterinary Manual - Giardiasis
- The Merck Online Medical Library - Giardiasis in humans
- Government of Alberta, Agriculture and Rural Development - Giardia in Dogs
References
- Fisher, M (2002) Endoparasites in the dog and cat: 2. Protozoa. In Practice, 24(3), 146-153.
- Taylor, M A and Webster, K A (1998) Recent advances in the diagnosis of Cryptosporidium, Toxoplasma, Giardia and other protozoa of veterinary importance. Research in Veterinary Science, 65, 183-193.
- Papasouliotis, K and Gruffydd-Jones, T (1996) Practical Approach to Diarrhoes in the Cat. In Practice, 18(5), 206-214.
- Tilley, L P and Smith, F W K (2007) The 5-minute Veterinary Consult (Fourth Edition) Blackwell Publishing.
- Rutgers, H C (1989) Diarrhoea in the Cat. In Practice, 11(4), 139-148.