Difference between revisions of "Escherichia coli"

From WikiVet English
Jump to navigation Jump to search
 
(38 intermediate revisions by 4 users not shown)
Line 1: Line 1:
<big><center>[[Enterobacteriaceae|'''BACK TO ENTEROBACTERIACEAE''']]</center></big>
+
{{review}}
<big><center>[[Bacteria|'''BACK TO BACTERIA''']]</center></big>
 
<big><center>[[Infectious agents and parasites|'''BACK TO INFECTIOUS AGENTS AND PARASITES''']]</center></big>
 
 
 
  
 
===''Eschericia coli'' (''E. coli'') overview===
 
===''Eschericia coli'' (''E. coli'') overview===
 
+
[[File:Ecoli.jpg|200px|right]]
 
*Member of ''Enterobacteriacae'' family of Gram-negative bacilli
 
*Member of ''Enterobacteriacae'' family of Gram-negative bacilli
 
*'''Facultative anaerobe'''
 
*'''Facultative anaerobe'''
Line 35: Line 32:
 
*Capsular polysaccharides produced by some strains prevent phagocytosis and interfere with complement
 
*Capsular polysaccharides produced by some strains prevent phagocytosis and interfere with complement
 
*Endotoxin is a lipolysaccharide component of the cell wall of Gram-negative bacteria, composed of lipid A, a core polysaccharide and various side chains
 
*Endotoxin is a lipolysaccharide component of the cell wall of Gram-negative bacteria, composed of lipid A, a core polysaccharide and various side chains
*'''Endotoxin''' is realeased when bacteria die, and causes endothelial damage leading to '''disseminated intravascular coagulation''' and e'''ndotoxic shock'''; it is also a '''pyrogen'''
+
*'''Endotoxin''' is realeased when bacteria die, and causes endothelial damage leading to [[Disseminated Intravascular Coagulation|'''disseminated intravascular coagulation''']] and '''endotoxic shock'''; it is also a '''pyrogen'''
 
*Enterotoxins, verotoxins and cytotoxic necrotising factors produced by many pathogenic ''E. coli''; these produce cell damage at their site of action
 
*Enterotoxins, verotoxins and cytotoxic necrotising factors produced by many pathogenic ''E. coli''; these produce cell damage at their site of action
 
*'''Alpha-haemolysin''' may increase iron availability for invading organisms
 
*'''Alpha-haemolysin''' may increase iron availability for invading organisms
 
*'''Siderophores''' are made by certain pathogenic strains, and are responsible for iron aquisition; they include aerobactin and enterobactin
 
*'''Siderophores''' are made by certain pathogenic strains, and are responsible for iron aquisition; they include aerobactin and enterobactin
 
  
 
==Extra-intestinal infection==
 
==Extra-intestinal infection==
Line 54: Line 50:
 
***Most common organism infecting urinary tract
 
***Most common organism infecting urinary tract
 
***Ascending infections of urinary tract
 
***Ascending infections of urinary tract
***Causes pyometra in the dog and cat and [[Kidney - Tubulointerstitial disease#Pyelonephritis|pyelonephritis]]
+
***Causes pyometra in the dog and cat and [[Pyelonephritis|pyelonephritis]]
 
***Cystitis in the bitch
 
***Cystitis in the bitch
 
***Prostatitis in dogs via opportunistic infection
 
***Prostatitis in dogs via opportunistic infection
Line 63: Line 59:
 
***Death within 24-48 hours during peracute disease
 
***Death within 24-48 hours during peracute disease
 
***Animals depressed with sunken eyes
 
***Animals depressed with sunken eyes
**[[Intestines - Catarrhal Enteritis#Colibacillosis|'''Colibacillosis''']]:  
+
**[[Colibacillosis|'''Colibacillosis''']]:  
 
***Avian:
 
***Avian:
 
****Septicaemia in newly-hatched chickens
 
****Septicaemia in newly-hatched chickens
Line 72: Line 68:
 
****Occurs in older birds via inhalation of ''E. coli'' in dust; respiratory infection spreads to the blood to cause acute colisepticaemia
 
****Occurs in older birds via inhalation of ''E. coli'' in dust; respiratory infection spreads to the blood to cause acute colisepticaemia
 
****Airsacculitis, pericarditis and perihepatitis during acute phase
 
****Airsacculitis, pericarditis and perihepatitis during acute phase
****Often secondary to virus or [[Mycoplasmas|''mycoplasma'']] infection or environmental stress
+
****Often secondary to virus or [[:Category:Mycoplasmas|''mycoplasma'']] infection or environmental stress
 
**'''Colisepticaemia''':
 
**'''Colisepticaemia''':
 
***Systemic disease in young calves, piglets, foals, lambs
 
***Systemic disease in young calves, piglets, foals, lambs
Line 79: Line 75:
 
***Virulence related to adhesive properties, complement resistance and ability for iron aquisition
 
***Virulence related to adhesive properties, complement resistance and ability for iron aquisition
 
***Ammonia, dust, viral infections and temperature changes enhance likelihood of disease
 
***Ammonia, dust, viral infections and temperature changes enhance likelihood of disease
**[[Stomach and Abomasum - Inflammation#Oedema Disease In The Pig|'''Oedema disease of pigs''']]:
+
**[[Oedema Disease|'''Oedema disease of pigs''']]:
***Acute, frequently fatal enterotoxaemia of weaned pigs
+
 
***Associated with particular haemolytic serotypes of ''E. coli''
 
***Verotoxin (Shiga toxin II e) released in the small intestine and carried in the bloodstream
 
***Haemolysin production
 
***Subcutaneous and subserosal oedema 
 
***Peracute disease affecting particulary healthy piglets
 
***Mortality rate 30%-90%
 
***Antimicrobial treatment effective if administered in time
 
 
**'''Watery mouth of lambs''':
 
**'''Watery mouth of lambs''':
 
***Affects lambs under three days old
 
***Affects lambs under three days old
Line 95: Line 84:
 
***Severe depression, anorexia, salivation and abdominal distension
 
***Severe depression, anorexia, salivation and abdominal distension
 
***Morbidity and mortality high
 
***Morbidity and mortality high
** [[Intestines - Inflammatory Bowel Disease And Related Conditions#Histiocytic Ulcerative Colitis|Histiocytic ulcerative colitis]] in the dog and cat.
+
** [[Inflammatory Bowel Disease#Histiocytic Ulcerative Colitis|Histiocytic ulcerative colitis]] in the dog and cat.
** Causes [[Peritoneal cavity - inflammatory#In dogs|peritonitis in dogs]] and [[Peritoneal cavity - inflammatory#In pigs|peritonitis in pigs]]
+
** Causes [[Peritonitis - Cats and Dogs|peritonitis in dogs]] and [[Peritonitis#In pigs|peritonitis in pigs]]
**Found in [[Bones - inflammatory#Osteomyelitis|osteomyelitis]]
+
**Found in [[Osteomyelitis|osteomyelitis]]
**Associated with neonatal [[Joints - inflammatory#In Cattle|polyarthritis of calves]]
+
**Associated with neonatal [[Infectious Arthritis#In Cattle|polyarthritis of calves]]
**Found in [[Joints - inflammatory#In Horses|arthritis of horses]]
+
**In [[Deep Pyoderma|deep pyoderma]]
 
+
**Found in [[Infectious Arthritis#In Horses|arthritis of horses]]
  
 
==Intestinal infection==
 
==Intestinal infection==
Line 106: Line 95:
 
*''E. coli'' is part of the flora of the large intestine, but is not usually found in the small intestine
 
*''E. coli'' is part of the flora of the large intestine, but is not usually found in the small intestine
 
*Some strains possess fimbrae which attach the bacteria to the small intestinal epithelium of particular animal species
 
*Some strains possess fimbrae which attach the bacteria to the small intestinal epithelium of particular animal species
*''E. coli'' may cause diarrhoea via attaching and effacing lesions, where bacteria adhere intimately to the enterocyte, and cause localised effacement of the brush border microvilli; the epithelial erosion causes [[General Pathology - Haemorrhage|Dysentery|dysentery]]
+
*''E. coli'' may cause diarrhoea via attaching and effacing lesions, where bacteria adhere intimately to the enterocyte, and cause localised effacement of the brush border microvilli; the epithelial erosion causes [[Haemorrhage#Dysentery|dysentery]]
 +
 
 +
 
 
*'''Enterotoxigenic ''E. coli'' (ETEC)''':
 
*'''Enterotoxigenic ''E. coli'' (ETEC)''':
 
**General:
 
**General:
*** Contributes to [[Intestines - Catarrhal Enteritis#Undifferentiated Neonatal Calf Diarrhoea|undifferentiated neonatal calf diarrhoea]], a mixed viral enteritis in calves, also known as enteric colibacillosis
+
*** Contributes to [[Calf Diarrhoea, Undifferentiated Neonatal|undifferentiated neonatal calf diarrhoea]], a mixed viral enteritis in calves, also known as enteric colibacillosis
 
***Causes scours in pigs, calves and lambs
 
***Causes scours in pigs, calves and lambs
 
***'Traveller's diarrhoea' in humans
 
***'Traveller's diarrhoea' in humans
Line 131: Line 122:
 
*****Activates guanylate cyclase in enteric epithelial cells, stimulating fluid secretion
 
*****Activates guanylate cyclase in enteric epithelial cells, stimulating fluid secretion
 
**Clinical signs:
 
**Clinical signs:
***The gut becomes distended with fluid and a [[Intestines - diarrhoea#Secretory Diarrhoeas|secretory diarrhoea]] which lasts several days results
+
***The gut becomes distended with fluid and a [[Diarrhoea#Secretory Diarrhoeas|secretory diarrhoea]] which lasts several days results
 
***Watery diarrhoea, dehydration, acidosis, death
 
***Watery diarrhoea, dehydration, acidosis, death
**Immunuty:
+
**Immunity:
 
***LT is antigenic
 
***LT is antigenic
 
***Immunity is developed via production of antibody to LT protein and fimbrial antigen
 
***Immunity is developed via production of antibody to LT protein and fimbrial antigen
 
***Parenteral vaccination of pigs and cattle protects offspring from scours via antibody production in the colostrum (passive immunity)
 
***Parenteral vaccination of pigs and cattle protects offspring from scours via antibody production in the colostrum (passive immunity)
 
***ST is not immunogenic; it is small, with only 19 amino acids
 
***ST is not immunogenic; it is small, with only 19 amino acids
*Enteropathogenic ''E. coli'' (EPEC):
+
 
**Attching and effacing strains of ''E. coli''
+
 
 +
*'''Enteropathogenic ''E. coli'' (EPEC)''':
 +
**Attaching and effacing strains of ''E. coli''
 
**Attach to small intestinal epithelial cells and cause necrosis of enterocytes and stunting and fusion of villi
 
**Attach to small intestinal epithelial cells and cause necrosis of enterocytes and stunting and fusion of villi
 
**Possess ''E. coli'' adherence factor plasmid
 
**Possess ''E. coli'' adherence factor plasmid
Line 146: Line 139:
 
**Intracellular calcium levels increase and production of protein kinase C causes loss of chloride ions and water from the intestinal epithelial cells
 
**Intracellular calcium levels increase and production of protein kinase C causes loss of chloride ions and water from the intestinal epithelial cells
 
**Diarrhoea results
 
**Diarrhoea results
*Enteroinvasive ''E. coli'':
+
 
 +
 
 +
*'''Enteroinvasive ''E. coli''''':
 
**Cause colisepticaemia in calves during their first week of life; occasionally in lambs, piglets and puppies
 
**Cause colisepticaemia in calves during their first week of life; occasionally in lambs, piglets and puppies
 
**Infection via ingestion or umbilicus; inadequate colostrum increases susceptibility
 
**Infection via ingestion or umbilicus; inadequate colostrum increases susceptibility
Line 156: Line 151:
 
**Abscesses, pneumonia in long term
 
**Abscesses, pneumonia in long term
 
**Death occurs in absense of treatment
 
**Death occurs in absense of treatment
*Enterohaemorrhagic ''E. coli'':
+
 
 +
 
 +
*'''Enterohaemorrhagic ''E. coli''''':
 
**Possibly carried by cattle
 
**Possibly carried by cattle
 
**Produce shiga-like toxin, a vero toxin
 
**Produce shiga-like toxin, a vero toxin
 
**Plasmid-coded fimbriae important for virulence
 
**Plasmid-coded fimbriae important for virulence
**Intimin produced allowing intimate attchment to intestinal epithelial cells
+
**Intimin produced allowing intimate attachment to intestinal epithelial cells
 
**Strains do not product LT or ST and are not enteroinvasive
 
**Strains do not product LT or ST and are not enteroinvasive
 
**Attaching and effacing lesions, unrelated to toxin production
 
**Attaching and effacing lesions, unrelated to toxin production
**Disseminated intravascular coagulation and thrombus formation  
+
**[[Disseminated Intravascular Coagulation|Disseminated intravascular coagulation]] and thrombus formation  
 
**''E. coli'' O157:H7 causes haemorrhagic collitis-haemolytic uraemic syndrome in humans
 
**''E. coli'' O157:H7 causes haemorrhagic collitis-haemolytic uraemic syndrome in humans
*Cytotoxin necrotising factor-producing ''E. coli''
+
 
 +
 
 +
*'''Cytotoxin necrotising factor-producing ''E. coli'''''
 
**Infrequently cause diarrhoea in calves, pigs and humans
 
**Infrequently cause diarrhoea in calves, pigs and humans
 
**Important virulence factors include toxin and fimbriae
 
**Important virulence factors include toxin and fimbriae
 +
 +
==in cattle==
 +
 +
 +
*Neonatal polyarthritis:
 +
 +
**[[Escherichia coli|'''''Coliforms''''']]
 +
***Localises in joints and meninges in severe non-fatal neonatal colibacillosis
 +
***May remain as chronic arthritis in larger joints
 +
 +
[[Category:Enterobacteriaceae]]
 +
[[Category:To_Do_-_Bacteria]]

Latest revision as of 11:30, 20 November 2012


Eschericia coli (E. coli) overview

Ecoli.jpg
  • Member of Enterobacteriacae family of Gram-negative bacilli
  • Facultative anaerobe
  • One of predominant bacterial species in colonic flora
  • Colonisation of intestinal tract from environmental sources shortly after birth
  • Abundant in the environment
  • Most strains have low virulence
  • Found in many non-specific, endogenous infections, eg. wound infections, upper respiratory tract infections, infections of the urinary tract, mammary glands and uterus and septicaemia
  • An enteropathogen, causing neonatal diarrhoea in young animals and enteric colibacillosis
  • Enterotoxigenic E. coli is the most common cause of diarrhoea in calves, lambs and pigs
  • Pathogenic strains possess virulence factors allowing colonisation of mucosal surfaces


E. coli characteristics

  • Usually motile with flagella and fimbriae
  • Oxidase negative (do not possess cytochrome C oxidase)
  • Grow on MacConkey agar (in presence of bile salts), producing pink colonies
  • 'Haemolytic activity on blood agar characteristic of certain strains
  • Lactose fermenter
  • Reduce nitrates to nitrites and ferment glucose to produce acid and gas
  • Possess a lipopolysaccharide (O) antigen, a flagellate (H) antigen, polysaccharide capsule (K) antigens and fimbrial (F) antigens
  • Epidemiological typing of E. coli uses antigen combinations, eg. O125:K12:H42


Pathogenesis

  • Virulence factors include capsules, endotoxin, enterotoxins and colonisation factors
  • Capsular polysaccharides produced by some strains prevent phagocytosis and interfere with complement
  • Endotoxin is a lipolysaccharide component of the cell wall of Gram-negative bacteria, composed of lipid A, a core polysaccharide and various side chains
  • Endotoxin is realeased when bacteria die, and causes endothelial damage leading to disseminated intravascular coagulation and endotoxic shock; it is also a pyrogen
  • Enterotoxins, verotoxins and cytotoxic necrotising factors produced by many pathogenic E. coli; these produce cell damage at their site of action
  • Alpha-haemolysin may increase iron availability for invading organisms
  • Siderophores are made by certain pathogenic strains, and are responsible for iron aquisition; they include aerobactin and enterobactin

Extra-intestinal infection

  • Soft tissue infections in adult animals
  • Pathogenesis:
    • Produces an alpha-haemolysin which may be cytotoxic
    • Iron aquisition system
    • K antigens prevent phagocytosis or mimic host antigens and resist complement
    • Fimbriae permit adhesion to mucosal surfaces
    • May enter blood to cause septicaemia
  • Clinical infections:
    • Urogenital tract infections
      • Most common organism infecting urinary tract
      • Ascending infections of urinary tract
      • Causes pyometra in the dog and cat and pyelonephritis
      • Cystitis in the bitch
      • Prostatitis in dogs via opportunistic infection
      • Colonisation of mucosa aided by fimbriae
    • Mastitis
      • Opportunistic infection of mammary glands of sows and cows
      • Endotoxaemia in the acute form often fatal
      • Death within 24-48 hours during peracute disease
      • Animals depressed with sunken eyes
    • Colibacillosis:
      • Avian:
        • Septicaemia in newly-hatched chickens
        • Infection enters via faecal contamination of the egg surface or via the ovary of the hen
        • Infection enters via the respiratory tract
        • A bacteraemia develops
        • Acute colisepticaemia, subacute fibrinopurulent serositis or chronic granulomatous disease of the viscera
        • Occurs in older birds via inhalation of E. coli in dust; respiratory infection spreads to the blood to cause acute colisepticaemia
        • Airsacculitis, pericarditis and perihepatitis during acute phase
        • Often secondary to virus or mycoplasma infection or environmental stress
    • Colisepticaemia:
      • Systemic disease in young calves, piglets, foals, lambs
      • Penetration of intestinal mucosa and entrance into the blood
      • Invasive strains survive the host defences
      • Virulence related to adhesive properties, complement resistance and ability for iron aquisition
      • Ammonia, dust, viral infections and temperature changes enhance likelihood of disease
    • Oedema disease of pigs:

Intestinal infection

  • E. coli is part of the flora of the large intestine, but is not usually found in the small intestine
  • Some strains possess fimbrae which attach the bacteria to the small intestinal epithelium of particular animal species
  • E. coli may cause diarrhoea via attaching and effacing lesions, where bacteria adhere intimately to the enterocyte, and cause localised effacement of the brush border microvilli; the epithelial erosion causes dysentery


  • Enterotoxigenic E. coli (ETEC):
    • General:
      • Contributes to undifferentiated neonatal calf diarrhoea, a mixed viral enteritis in calves, also known as enteric colibacillosis
      • Causes scours in pigs, calves and lambs
      • 'Traveller's diarrhoea' in humans
    • Pathogenesis:
      • Oral infection, intestinal colonisation and toxin production
      • Fimbrial antigen or colonisation factor antigens (CFAs)determine species specificity
      • Fimbrial adhesins allow bacteria to attach to mucosal surfacesin the small intestine and lower urinary tract; this prevents expulsion by peristalsis and flushing of urine
      • K88 (F4) is associated with adhesion to the small intestinal mucosa of pigs
      • K99 (F5) associated with adhesion in pigs and cattle (these fimbrial adhesins were originally thought to be capsular (K) antigens)
      • The fimbriae are encoded by plasmids
      • These strains carry a plasmid which encodes an enterotoxin
      • Two types of enterotoxin: heat-labile (LT) and heat-stable (ST) toxins
      • The plasmids which produce these toxins are responsible for the pathogenicity of these strains
        • LT
          • An oligometric toxin composed of an enzymatically-active A subunit (30KDa; 2 fragments - A1 and A2) and 5 identical B subunits (12KDa) forming the binding portion (B oligomer)
          • It attaches to the brush border of the epithelial cells of the small intestine
          • Causes ADP-ribosylation of the stimulatory subunit of guanine nucleotide binding proteins of the adenylate cyclase complex in eukaryotic cell membranes
          • This causes irreversible activation of adenylate cyclase in target cells
          • This raises the cAMP level and causes hypersecretion of water and chloride ions into the lumen of the small intestine and inhibits reabsorption of sodium
        • ST
          • Activates guanylate cyclase in enteric epithelial cells, stimulating fluid secretion
    • Clinical signs:
      • The gut becomes distended with fluid and a secretory diarrhoea which lasts several days results
      • Watery diarrhoea, dehydration, acidosis, death
    • Immunity:
      • LT is antigenic
      • Immunity is developed via production of antibody to LT protein and fimbrial antigen
      • Parenteral vaccination of pigs and cattle protects offspring from scours via antibody production in the colostrum (passive immunity)
      • ST is not immunogenic; it is small, with only 19 amino acids


  • Enteropathogenic E. coli (EPEC):
    • Attaching and effacing strains of E. coli
    • Attach to small intestinal epithelial cells and cause necrosis of enterocytes and stunting and fusion of villi
    • Possess E. coli adherence factor plasmid
    • An adhesin, intimin is required for attachment to enterocytes
    • Secrete signalling proteins that activate a tyrosine kinase, causing rearrangement of cytoskeletal proteins and effacement of microvilli
    • Intracellular calcium levels increase and production of protein kinase C causes loss of chloride ions and water from the intestinal epithelial cells
    • Diarrhoea results


  • Enteroinvasive E. coli:
    • Cause colisepticaemia in calves during their first week of life; occasionally in lambs, piglets and puppies
    • Infection via ingestion or umbilicus; inadequate colostrum increases susceptibility
    • Invade epithelial cells of small intestine by inducing endocytosis
    • Traverse gut wall to lamina propria and enter lymphatics
    • Resistant to complement-mediated killing
    • Bacteraemia or septicaemia and endotoxaemia
    • Widespread petechial haemorrhages of organs and serosa
    • Abscesses, pneumonia in long term
    • Death occurs in absense of treatment


  • Enterohaemorrhagic E. coli:
    • Possibly carried by cattle
    • Produce shiga-like toxin, a vero toxin
    • Plasmid-coded fimbriae important for virulence
    • Intimin produced allowing intimate attachment to intestinal epithelial cells
    • Strains do not product LT or ST and are not enteroinvasive
    • Attaching and effacing lesions, unrelated to toxin production
    • Disseminated intravascular coagulation and thrombus formation
    • E. coli O157:H7 causes haemorrhagic collitis-haemolytic uraemic syndrome in humans


  • Cytotoxin necrotising factor-producing E. coli
    • Infrequently cause diarrhoea in calves, pigs and humans
    • Important virulence factors include toxin and fimbriae

in cattle

  • Neonatal polyarthritis:
    • Coliforms
      • Localises in joints and meninges in severe non-fatal neonatal colibacillosis
      • May remain as chronic arthritis in larger joints