Difference between revisions of "In Vitro Fertilization - Anatomy & Physiology"
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** Follicular fluid forcefully returned to the [[Follicles - Anatomy & Physiology|follicle]]. | ** Follicular fluid forcefully returned to the [[Follicles - Anatomy & Physiology|follicle]]. | ||
** Repeated 2-3 times to dislodge [[Oogenesis - Anatomy & Physiology|oocytes]]. | ** Repeated 2-3 times to dislodge [[Oogenesis - Anatomy & Physiology|oocytes]]. | ||
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* Transvaginal Aspiration in the Mare: | * Transvaginal Aspiration in the Mare: | ||
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** [[Ovary - Anatomy & Physiology|Ovary]] transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[Follicles - Anatomy & Physiology|follicle]] can be visualized. | ** [[Ovary - Anatomy & Physiology|Ovary]] transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[Follicles - Anatomy & Physiology|follicle]] can be visualized. | ||
** Hypodermic needle is advanced through the [[Vagina and Vestibule - Anatomy & Physiology|vaginal wall]] into the antral follicle. | ** Hypodermic needle is advanced through the [[Vagina and Vestibule - Anatomy & Physiology|vaginal wall]] into the antral follicle. | ||
− | ** Follicular fluid containing the [[Oogenesis - Anatomy & Physiology| | + | ** Follicular fluid containing the [[Oogenesis - Anatomy & Physiology|oocyte]] is aspirated under constant vacuum. |
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− | + | * Aspirated [[Oogenesis - Anatomy & Physiology|oocytes]] are placed in a culture vessel with [[Sperm in the Female Tract - Anatomy & Physiology#Capacitation|capacitated]] spermatozoa. | |
+ | ** Could also be cultured in a 'nurse animal' in vivo. | ||
* Embryos are incubated for a further period to the appropriate stage for transfer. | * Embryos are incubated for a further period to the appropriate stage for transfer. | ||
* Embryos transferred to recipient female. | * Embryos transferred to recipient female. | ||
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* Embryos transferred around the [[Blastocyst Embryonic Development - Anatomy & Physiology|blastocyst]] stage. | * Embryos transferred around the [[Blastocyst Embryonic Development - Anatomy & Physiology|blastocyst]] stage. | ||
* Success rates are improving. | * Success rates are improving. | ||
− | * Embryos can be frozen, eliminating the need for tight | + | * Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations. |
* Increases incidence of [[Large_Offspring|large offspring syndrome]]. | * Increases incidence of [[Large_Offspring|large offspring syndrome]]. | ||
Revision as of 13:27, 2 November 2012
Oocyte Collection
- Donor is superovulated to provide large numbers of mature follicles.
- Pre-ovulatory follicles are collected by a non-surgical procedure involving ultrasound guided aspiration of the follicular content.
- Cumulus-Oocyte-Complexes are matured and fertilized in vitro.
- Direct follicle aspiration:
- Transvaginal Aspiration in the Mare:
- Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the rectum.
- Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.
- Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized.
- Hypodermic needle is advanced through the vaginal wall into the antral follicle.
- Follicular fluid containing the oocyte is aspirated under constant vacuum.
- Aspirated oocytes are placed in a culture vessel with capacitated spermatozoa.
- Could also be cultured in a 'nurse animal' in vivo.
- Embryos are incubated for a further period to the appropriate stage for transfer.
- Embryos transferred to recipient female.
- Can be transferred at the correct stage of the recipient's oestrous cycle.
- Embryos transferred around the blastocyst stage.
- Success rates are improving.
- Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations.
- Increases incidence of large offspring syndrome.