Line 43: |
Line 43: |
| ||<font color="white"> <big> | | ||<font color="white"> <big> |
| *'''''Excitation''''' | | *'''''Excitation''''' |
− | ||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]] | + | ||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]] |
| |- | | |- |
| |<big>'''What are the fluorescent dyes commonly known as?''' | | |<big>'''What are the fluorescent dyes commonly known as?''' |
| ||<font color="white"> <big> | | ||<font color="white"> <big> |
| *'''''Fluorochromes''''' | | *'''''Fluorochromes''''' |
− | ||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]] | + | ||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]] |
| |- | | |- |
| |<big>'''What are the advantages of indirect staining?''' | | |<big>'''What are the advantages of indirect staining?''' |
Line 56: |
Line 56: |
| *'''''Relative expressions of different antigens in the same cell can be tested''''' | | *'''''Relative expressions of different antigens in the same cell can be tested''''' |
| *'''''Loss of antibody during conjugation is limited''''' | | *'''''Loss of antibody during conjugation is limited''''' |
− | ||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]] | + | ||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]] |
| |- | | |- |
| |<big>'''What technique is used to provide a quantitative measure of immunofluorescence?''' | | |<big>'''What technique is used to provide a quantitative measure of immunofluorescence?''' |
| ||<font color="white"> <big> | | ||<font color="white"> <big> |
| *'''''Flow cytometry''''' | | *'''''Flow cytometry''''' |
− | ||[[ELISA testing|<span title="Answer article">Link to Answer Article</span>]] | + | ||[[Immunofluorescence|<span title="Answer article">Link to Answer Article</span>]] |
| + | |} |
| + | |
| + | ==<font color="purple">Radioimmunoassay</font>== |
| + | {| border="3" cellpadding="8" |
| + | !width="400"|'''Question''' |
| + | !width="400"|'''Answer''' |
| + | !width="150"|'''Article''' |
| + | |- |
| + | |<big>'''What are radioimmunoassays commonly used to detect?''' |
| + | ||<font color="white"> <big> |
| + | *'''''Hormone levels in blood and tissue fluids''''' |
| + | *'''''Serum proteins''''' |
| + | *'''''Drugs''''' |
| + | *'''''Vitamins''''' |
| + | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] |
| + | |- |
| + | |<big>'''What is the most common isotope used for radioimmunoassay?''' |
| + | ||<font color="white"> <big> |
| + | *'''''Iodine-125''''' |
| + | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] |
| + | |- |
| + | |<big>'''Why is it important to measure the amount of antibody that binds to 50% of the labelled antigen?''' |
| + | ||<font color="white"> <big> |
| + | *'''''Ensures the number of epitopes present exceeds number of antibody-binding sites''''' |
| + | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] |
| + | |- |
| + | |<big>'''What are the disadvantages of the radioimmunoassay?''' |
| + | ||<font color="white"> <big> |
| + | *'''''Substances used are radioactive''''' |
| + | *'''''Gamma radiation requires special counting equipment''''' |
| + | *'''''Iodine naturally accumulates in the thyroid gland''''' |
| + | ||[[Radioimmunoassay|<span title="Answer article">Link to Answer Article</span>]] |
| + | |
| + | |} |
| + | ==<font color="purple">Agglutination</font>== |
| + | {| border="3" cellpadding="8" |
| + | !width="400"|'''Question''' |
| + | !width="400"|'''Answer''' |
| + | !width="150"|'''Article''' |
| + | |- |
| + | |<big>'''What are the antibodies that produce agglutination known as?''' |
| + | ||<font color="white"> <big> |
| + | *'''''Agglutinins''''' |
| + | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] |
| + | |- |
| + | |<big>'''What constitutes the end of the agglutination test?''' |
| + | ||<font color="white"> <big> |
| + | *'''''The last tube showing distinct agglutination''''' |
| + | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] |
| + | |- |
| + | |<big>'''What does the titre represent?''' |
| + | ||<font color="white"> <big> |
| + | *'''''The number of antibodies per unit volume of serum''''' |
| + | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] |
| + | |- |
| + | |<big>'''What is the prozone effect?''' |
| + | ||<font color="white"> <big> |
| + | *'''''An inhibition of agglutination caused by an excess of antibodies''''' |
| + | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] |
| + | |- |
| + | |<big>'''What agglutination test is often used to test for rhesus disease?''' |
| + | ||<font color="white"> <big> |
| + | *'''''Coombs test''''' |
| + | ||[[Agglutination|<span title="Answer article">Link to Answer Article</span>]] |