Piroplasmida

PARASITES PROTOZOA

Introduction

The piroplasms are a group of blood-bourne protozoa which are transmitted by ticks. The two species most of veterinary importance are Babesia, Cytauxzoon and Theileria.

Piroplasms are apicomplexan protozoa which inhabit erythrocytes, and sometimes other cells of vertebrates, but do not form pigment from haemoglobin. All piroplasms are small and round or pear-shaped (erythrocyte forms) and are parasitic on fish, amphibians, birds and mammals.

Babesia

Babesia Life Cycle Diagram - Dennis Jacobs & Mark Fox RVC

• Infects a wide range of host species in different areas of the world

• Babesiosis has severe effects on cattle production in parts of the world
  • Prevents European breeds from being successful in tropical regions where ticks are endemic.
  • Occurs sporadically in the UK and Ireland causing losses of around £8 million per year

Life Cycle

• Both trans-stadial and trans-ovarian occurs

• Each female tick produces 3000 eggs

• The tick is the definitive host

• May cause haemolysis and haemoglobin pigmentation.

Epidemiology

Theileria

Theileria parva Life Cycle Diagram - Dennis Jacobs & Mark Fox RVC

• Main species of veterinary importance is Theileria parva
  • Causes East Coast Fever
    • Severe, proliferative lymphatic disease of cattle
    • Central and Eastern Africa
    • Transmitted by Rhipicephalus appendiculatus
    • Trans-stadial transmission

• Other Theileria species causes production losses in cattle and sheep in the Middle East, Mediterranean and in Northern Africa

Life Cycle

• Incubation phase lasts 1 week

• Lymphoblast proliferation
  • Local lymph node first infected then spreads through body
  • Occurs in week two

• Lymphoid depletion
  • Lymphocytes killed
  • Decreases lymphopoiesis
  • Occurs in week 3

• Total incubation period takes about 18 days

Diagnosis

• Clinical signs
  • Pyrexia
  • Enlarged local lymph node
    • Usually parotid lymph node as Rhipicephalus appendiculatus feeds in the ear
  • Loss of condition

• Examine Giemsa stained smears of:
  • Local lymph node aspirated for schizonts
  • Blood smears for pioplasms in red blood cells

• Post-mortem
  • Pulmonary oedema
  • Gut mucosal haemorrhages
  • Lymph node and spleen cellular atrophy

Control

• Integrated control of both the tick and vector
  • Vaccination and ectoparasiticides

• Current vaccination is live unattentuated
  • Contains frozen stabilate of ground up tick gut containing infective sporozoites
  • Long lasting oxytetracycline administered at the same time to slow down schizogony giving the immune response time to develop

Cytauxzoon felis

• Cytauxzoon is classified in the order Piroplasmida and family Theileriidae
  • This family has both an erythrocytic and a tissue (leukocytic) phase

• The Babesiidae, a related family, is characterized by having a primarily erythrocytic phase in the mammalian host
  • Its morphological features are indistinguishable from the erythrocytic form of Cytauxzoon

• Cytauxzoon felis, B. equi, and B. rodhaini have been linked to both the babesias and theilerias by RNA gene sequence analysis
  • It has been suggested that these organisms be reclassified within a separate family

Life Cycle

• Large schizonts of C. felis develop in macrophages
  • In Theileria the exoerythrocytic stage occurs primarily within lymphocytes

• In C. felis, schizonts develop within mononuclear phagocytes, initially as indistinct vesicular structures and later as large, distinct nucleated schizonts that actively undergo division by true schizogony and binary fission

• Later in the course of the disease, schizonts develop buds (merozoites) that separate and eventually fill the entire host cell

• Each schizont may contain numerous merozoites
  • Ultrastructurally, schizonts lack a parasitophorous vacuole, and individual merozoites possess rhoptries

• The host cell ruptures, releasing merozoites into the tissue fluid and blood

• Merozoites are then believed to enter erythrocytes to form the intraerythrocytic stage

• Merozoites appear in macrophages one to three days before they are observed in erythrocytes

Pathogenicity

• Ticks are implicated as the natural vector for Cytauxzoon
  • Most cases of infection have been associated with the presence of these parasites on the hosts
  • Experimentally, Dermacentor variabilis can transmit the organism from bobcats to domestic cats. In a white tiger that developed a natural, fatal infection in Florida, two female Lone Star ticks (Amblyomma americanum) were present on the inguinal skin.

• Clinically, the disease in cats is characterized by fever, depression, dyspnea, anorexia, lymphadenopathy, anaemia, and icterus leading to death in three to six days

• Gross findings include pale or icteric mucous membranes, petechiae and ecchymoses in the lung, heart, lymph nodes and on mucous membranes, splenomegaly, lymphadenomegaly, and hydropericardium

• Microscopically, numerous large schizonts are present within the cytoplasm of endothelial-associated macrophages
  • Infected macrophages become markedly enlarged (up to 75μm) and may occlude the lumens of numerous vessels of many tissues, especially the lungs
  • Minimal inflammatory reaction is present in tissues

Diagnosis

• Merozoites within erythrocytes, best seen on peripheral blood or tissue impressions, are variable in morphology and can occur as round, oval, or signet ring-shaped bodies
  • Are 1-5 micrometers in diameter
  • Small, peripherally placed basophilic nucleus

• Organisms that must be distinguished from the intraerythrocytic phase of C. felis include Babesia and Hemobartonella
  • The blood stage may appear similar to the ring forms of Hemobartonella and to the piriforms of Babesia
  • Unlike Cytauxzoon, babesiosis and hemobartonellosis do not have a tissue stage of infection

• Differential diagnosis for the tissue phase of cytauxzoonosis includes other small (less than 5 μm), intrahistiocytic organisms such as Toxoplasma, Leishmania and Histoplasma