Haematopoiesis

Introduction

Also referred to as haemopoiesis or hemopoiesis and is the process of blood cell formation. All blood cells are derived from the initial pluripotent stem cell (PPSC) which gives rise to colony forming units (CFU). These CFU's further differentiate to give rise to the various blood cells found.

Development

Blood cells ©RVC 2008 Avian Blood cells ©RVC 2008

PPSC’s initially divide and differentiate into multipotential haematopoietic stem cells. Multipotential haematopoietic stem cells only have the ability to mature into a single cell lineage, either myeloid progenitors (granulocytes, erythroblasts, macrophages and megakaryocytes) or lymphoid progenitors. The development of blood cells from the multipotential haematopoietic stem cell into the final mature cell is categorised into:

• The formation of erythrocytes (Erythropoiesis)
• The formation of white blood cells (Leukopoiesis)
• The formation of platelets (Thrombopoiesis)

White blood cells are further classified by the lineage of the white blood cell being formed. Thus the development of white blood cells is further categorised into:

• Formation of neutrophils, eosinophils and basophils (Granulopoiesis)
• Formation of B cells and T cells (Lymphopoiesis)
• Formation of monocytes and macrophages (See here)

Stages

Fetal

Yolk Sac Phase

Blood islands from the mesoderm form in the yolk sac early in gestation. They provide primitive erythrocytes to support the developing embryo. These blood islands also give rise to the haematopoietic stem cells.

Haematopoiesis also occurs within the embryo at a region called the aorta-gonad-mesonephros region (AGM).

Hepatic Phase

Back to Primary Lymphoid Tissue

As embryological development continues haematopoiesis shifts from the yolk sac and AGM to the foetal liver (and spleen). Haematopoietic areas form in the liver which become the main haematopoietic organ in the body. Erythropoiesis is the dominant process but some leukopoiesis occurs so the foetal liver can be considered a primary lymphoid organ.

For pathology of the foetal liver see here.

Bone marrow phase

Haematopoiesis starts to occur in the bone marrow later in gestation, and the spleen becomes the main erythrocyte producing organ during the transitional phase from liver to bone marrow.

Adult

Haematopoiesis occurs in bone marrow and maturation of lymphocytes in lymphoid tissues. In the bone marrow the haematopoietic cells are supported by stromal cells which generate the correct environments to cause the growth and development of the different blood cell types.

Lineages

The lineage of a blood cell is based on the multipotential stem cell that the cell is derived from.

• Lymphoid cells are derived from the multipotential lymphoid stem cells
• Cells derived from the multipotential myeloid stem cells are either:
  • Erythroid lineage (forming erythrocytes) or
  • Myeloid lineage (forming neutrophils, monocytes, macrophages, eosinophils, basophils and platelets)

Colony Forming Units

CFU-L	(multipotential lymphoid stem cell forming T cells, B cells and NK cells)
CFU-GEMM	(multipotential myeloid stem cell, forming granulocytes, erythroblasts, macrophages and megakaryocytes)
CFU-E	(forming erythrocytes)
CFU-GM	(forming neutrophils and monocytes/macrophages)
CFU-Eo	(forming eosinophils)
CFU-Ba	(forming basophils)
CFU-Mast	(forming mast cells)
CFU-Meg	(forming platelets)

CFU Development

Pluripotential Stem Cell (PPSC)
Multipotential myeloid stem cell (CFU-GEMM)							Multipotential lymphoid stem cell (CFU-L)
Erythroid CFU (CFU-E)	Megakaryocyte CFU (CFU-Meg)	Granulocyte CFU (CFU-GM)		Basophil CFU (CFU-Ba)	Eosinophil CFU (CFU-Eo)	Mast Cell CFU (CFU-Mast)	-
		Neutrophil CFU (CFU-G)	Monocyte CFU (CFU-M)
Erythrocyte	Megakaryocyte	Neutrophil	Monocyte	Basophil	Eosinophil	Mast Cell	T cell, B cell & NK cell