Difference between revisions of "Vaccines"

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{{toplink
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[[Image:Syringe.jpg|right|thumb|200px|<p>'''Syringe'''</p>Source: Wikimedia Commons; Author: ZaldyImg (2008)]]
|linkpage =Immunology - WikiBlood
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==Introduction==
|linktext =IMMUNOLOGY
+
Why do we vaccinate animals?  
|thispagemap= Vaccines(Concept Map) - WikiBlood
 
|pagetype =Blood
 
}}
 
 
 
==Why Vaccinate?==
 
 
 
 
*To protect against infectious diseases
 
*To protect against infectious diseases
 +
*Where there is no effective treatment once infected e.g. FeLV, FIV
 +
*Where disease is life-threatening e.g. Canine Parvovirus
 +
*To prevent the spread of disease by virus excretion e.g. Rabies, FMDV
  
*Where there is no effective treatment once infected
+
The goal is to vaccinate 90% of the population to reduce the amount of '''endemic''' virus until no new infections occur. Once the disease risk is low, vaccination can be replaced by an eradication or quarantine programme.
**E.g. [[Immunodeficiencies - WikiBlood#Feline Leukaemia Virus (FeLV)|FeLV]], FIV
 
 
 
*Where disease is life-threatening
 
**E.g. Canine Parvovirus
 
 
 
*To prevent the spread of disease by virus excretion
 
**E.g. Rabies, FMDV
 
 
 
*The goal is to vaccinate 90% of the population to reduce the amount of '''endemic''' virus until no new infections occur
 
 
 
*Once the disease risk is low, vaccination can be replaced by an eradication or quarantine programme  
 
  
 
==How do vaccines work?==
 
==How do vaccines work?==
 +
Vaccination induces an immunological memory of the infectious organism. High levels of [[T cell differentiation#Cytotoxic T-Cells|cytotoxic T cells]] and neutralising [[Immunoglobulins - Overview|antibody]] are activated 24 - 48 hours post vaccination as a [[B cell differentiation#Secondary T Cell Dependent Response|secondary response]] (instead of 4-10 days later as a [[B cell differentiation#T-Cell Dependent Response|primary response]]). Neutralising [[Immunoglobulins|antibody]] then blocks the attachment of the infectious organism to host cell receptors.
  
*Vaccination sets up memory to the viral infection
+
'''Endogenous vaccines''' cause antigens to be made as new proteins by the cell, bacterium or virus and involves [[Major Histocompatability Complexes#MHC I|MHC class I]] processing live virus, recombinant virus or DNA vaccines.
  
*High levels of [[T cell differentiation - WikiBlood#Cytotoxic T-Cells|cytotoxic T cells]] and neutralising [[Immunoglobulins - WikiBlood|antibody]] are activated in 1-2 days as a [[B cell differentiation - WikiBlood#Secondary T Cell Dependent Response|secondary response]] (instead of 4-10 days as a [[B cell differentiation - WikiBlood#T-Cell Dependent Response|primary response]])
+
'''Exogenous vaccines''' are when the antigen is processed from the outside by endocytosis without any new proteins being made by the host cell. This involves [[Major Histocompatability Complexes#MHC II|MHC class II]] processing inactivated and subunit vaccines.
  
*The infection is therefore prevented from taking hold causing lesions to develop
+
==Route of Administration==
 +
*Usually by subcutaneous injection for '''systemic''' protection. Some vaccines such as the [[Vaccinations_for_Rabbits#Myxomatosis_Vaccination|myxomatosis]] vaccine NobivacTM Myxo (Intervet UK Ltd) require an intradermal injection as part of the administration procedure.
 +
*For a localised '''mucosal''' immune response, intranasal administration is required ([[Immunoglobulin A|IgA]]) e.g. kennel cough vaccine.
  
*Neutralising [[Immunoglobulins - WikiBlood|antibody]] blocks the attachment of virus to host cell receptors
+
==Vaccination Options==
 
+
[[Image:Passive Immunisation.jpg|thumb|right|200px|Passive Immunisation - Copyright nabrown RVC]]
*'''Endogenous vaccines''' are where the [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]] are made as new proteins by the cell, bacterium or virus
 
**Involves [[MHC - WikiBlood#MHC I|MHC class I]] processing
 
**E.g. live virus, recombinant virus and DNA vaccines
 
 
 
*'''Exogenous vaccines''' are when the [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]] is processed from the outside by endocytosis without any new proteins being made by the host cell
 
**involves [[MHC - WikiBlood#MHC II|MHC class II]] processing
 
**E.g. Inactivated and subunit vaccines
 
 
 
==How do we vaccinate?==
 
 
 
*Usually by subcutaneous injection for '''systemic''' protection ([[Immunoglobulin G - WikiBlood|IgG]])
 
 
 
*For '''mucosal''' immune response, intranasal administration is best ([[Immunoglobulin A - WikiBlood|IgA]])
 
 
 
==What do we vaccinate with?==
 
[[Image:Passive Immunisation.jpg|thumb|right|150px|Passive Immunisation - Copyright nabrown RVC]]
 
 
===Passive immunisation===
 
===Passive immunisation===
  
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'''Disadvantages:'''
 
'''Disadvantages:'''
*Short duration of action
+
*Short duration of action; temporary protection is obtained by the administration of preformed [[Immunoglobulins - Overview|antibody]] from another individual of the same or of a different species. The acquired antibodies are used in combination with [[Adaptive Immune System - Overview#Antigen Recognition|antigen]], and catabolised by the body, meaning protection is gradually lost over time
**Temporary protection by the administration of preformed [[Immunoglobulins - WikiBlood|antibody]] from another individual of the same or of a different species
+
*Injection of antiserum may cause an [[Adverse Drug Reactions|allergic response]]
**The acquired [[Immunoglobulins - WikiBlood|antibodies]] are used in combination with [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]], and catabolised by the body, meaning protection is gradually lost
+
*Antiserum contains many antibodies, not just the specific [[Immunoglobulins|antibodies]] needed
*Injection of antiserum may cause an [[Allergic diseases - WikiClinical|allergic response]]
 
*Antiserum contains many [[Immunoglobulins - WikiBlood|antibodies]], not just the specific [[Immunoglobulins - WikiBlood|antibodies]] needed
 
  
'''Types of [[Immunoglobulins - WikiBlood|antibodies]] administered:'''
+
'''Types of antibodies administered:'''
*Maternally-derived [[Immunoglobulins - WikiBlood|antibodies]] in [[Materno-fetal immunity - WikiBlood#Passive transfer via colostrum|colostrum]]
+
*Maternally-derived antibodies in [[Materno-Fetal Immunity - Introduction#Passive transfer via colostrum|colostrum]] when there is a [[Failure of Passive Transfer|failure of passive transfer]] of [[Immunoglobulin G]]
*Antiserum (artificial)
+
*Antiserum  
**The [[Immunoglobulins - WikiBlood|antibodies]] are used in combination with [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]] (and often an [[Vaccines - WikiBlood#Adjuvants|adjuvant]]) which is injected into the host animal
+
**The antibodies are used in combination with [[Adaptive Immune System - Overview#Antigen Recognition|antigen]] (and often an adjuvant) which is injected into a host animal
**The immune system of that animal synthesises [[Immunoglobulins - WikiBlood|antibodies]]
+
**The immune system of that animal synthesises antibodies
**Repeated injections at intervals increases the total [[Immunoglobulins - WikiBlood|antibody]] production
+
**Repeated injections at intervals increases total [[Immunoglobulins - Overview|antibody]] production
**The immunised animal is bled and the serum collected which contains the newly made [[Immunoglobulins - WikiBlood|antibodies]]. The serum is called '''antiserum'''.
+
**The immunised animal is bled and the serum collected which contains the newly made antibodies. The serum is called '''antiserum'''.
 
**The serum can then be injected into a different animal to confer passive immunisation
 
**The serum can then be injected into a different animal to confer passive immunisation
  
*Example of when passive immunisation is used:
 
**Suspect tetanus
 
  
'''Passive Immunotherapy with Antibody'''
+
Examples of passive immunisation:
 
{| style="width:60%; height:200px" border="1" align=left
 
{| style="width:60%; height:200px" border="1" align=left
 
!INFECTION
 
!INFECTION
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| Used
 
| Used
 
| Not used
 
| Not used
| Post-exposure to vaccine
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| Post-exposure to virus
 
|}
 
|}
 
<Br clear="left">
 
<Br clear="left">
 
<br>
 
<br>
[[Image:Active Immunisation.jpg|thumb|right|150px|Active Immunisation - Copyright nabrown RVC]]
+
[[Image:Active Immunisation.jpg|thumb|right|200px|Active Immunisation - Copyright nabrown RVC]]
  
 
===Active immunisation===
 
===Active immunisation===
*Administer [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]] so the patient develops its own [[Immunoglobulins - WikiBlood|antibodies]] to protect against disease
+
Active immunisation requires the administration of antigen so the patient develops their own antibodies to protect against disease. Suitable antigens include:
**Living organisms
+
*Living organisms
**Dead organisms
+
*Dead organisms
**Toxoids
+
*Toxoids
**Subunit antigens
+
*Subunit antigens
**DNA
+
*DNA
  
 
'''Advantages'''
 
'''Advantages'''
*Long duration of action  
+
*Long duration of action; once antibody is produced against the antigen, [[B cell differentiation#Memory cells|memory cells]] are formed which continue circulating in the body
**Once [[Immunoglobulins - WikiBlood|antibody]] is produced against the [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]], [[B cell differentiation - WikiBlood#Memory cells|memory cells]] are formed which continue circulating in the body
 
**For further information on memory cells click [[B cell differentiation - WikiBlood|here]]
 
  
 
'''Disadvantages'''
 
'''Disadvantages'''
*Delay in protection
+
*The host's immune system needs to evoke an immune response against the antigen which can take a few days
**The host's immune system needs to evoke an immune response against the [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]] which can take a few days
+
*Can require two or more doses to be effective; the first dose initiates the '''priming''' reaction where antibody production ceases after a few weeks, but the second and subsequent doses create memory cells which remain in the circulation for a much longer period of time.
**For further information on the [[Immunoglobulins - WikiBlood|antibody]] response click [[Adaptive Immune System - WikiBlood|here]]
 
 
 
*Often needs two or more doses  
 
**The first dose initiates the '''priming''' reaction where [[Immunoglobulins - WikiBlood|antibody]] production ceases after a few weeks, but the second and subsequent doses create [[B cell differentiation - WikiBlood#Memory cells|memory cells]] which remain in the circulation for a much longer period of time
 
**For further information on the T cell independent and dependent responses click [[B cell differentiation - WikiBlood#T-Cell Dependent and Independent Responses|here]]
 
 
 
==What antigen(s) do we use in the vaccine?==
 
  
 +
==Vaccine Antigens==
 +
Potential antigenic substances include:
 
===Whole Organism===
 
===Whole Organism===
 +
'''Live Attenuated (LA) vaccines''' include the organism but in an altered form - virulent organisms cannot be used as vaccines as they have the potential to cause disease. Virulence is reduced by growing the organism in altered conditions (e.g. in cells or eggs), so that it is less able to replicate when introduced into the host, and is therefore less likely to cause disease. Virulence can also be reduced by genetic engineering, or by using naturally occurring avirulent strains.
  
*Live attenuated organism
 
**Virulent organisms cannot be used as vaccines as they would cause disease
 
**Virulence is reduced by growing the organism in altered conditions (e.g. in cells or eggs), so that it is less able to replicate when introduced to the host, and therefore less likely to cause disease
 
**Produces a superior response to disease than using killed organisms as the dose of [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]] is larger and more sustained
 
**Virulence can also be reduced by genetic engineering
 
**Naturally occurring avirulent strains can also be used
 
**Response takes place at the site of natural infection, producing a greater local response than with killed organism vaccines
 
**E.g. The current vaccine for Tuberculosis (called BCG) contains an attenuated form of a mycobacteria
 
**E.g. Vaccines for Leishmaniasis
 
**E.g. Vaccines for parainfluenza virus 3 of calves is developed to be temperature-sensitive so that it grows at 34 C in the upper respiratory tract but not at 38 C in the lungs
 
 
*Killed inactivated organism or toxin (toxoid)
 
**Virulent and toxic organisms cannot be used as vaccines as they would cause disease
 
**Organisms can be killed using radiation or chemicals so that they still possess the [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigens]] to stimulate an immune response, but the organisms are unable to replicate inside the host
 
**Toxins are inactivated to produce a toxoid which will still have the [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigens]] needed to produce an immune response but will not be harmful to the host
 
**Needs two doses (for an explanation on the [[Lymphocytes - WikiBlood#T cells|T cell]] response click [[B cell differentiation - WikiBlood#T-Cell Dependent and Independent Responses|here]])
 
**1:4000 formaldehyde is the current preparation
 
**Inactivants containing azuridines and beta propiolactone are being developed which do not leave a persistent infectious viral fraction (like formaldehyde)
 
 
===Subunit Vaccine (part of the organism)===
 
 
*Purified protein
 
**Single envelope protein separated from a purified virus by detergent then centrifuged (traditional method)
 
**Genetic engineering can now make single protein vaccines
 
 
*Recombinant or synthetic protein
 
**The gene for the [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]] required is inserted into a virus vector or cloned into bacteria allowing endogenous expression
 
**Small [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigens]], such as peptides, can be synthetically produced
 
**E.g. Being developed constantly to fight the Influenza viruses
 
**E.g. Canary pox vaccines encoding rabies or FeLV spike proteins (canary pox is safe as it undergoes incomplete replication in mammalian skin cells)
 
 
*DNA coding for proteins ([[Adaptive Immune System - WikiBlood#Antigen Recognition|antigens]])
 
**Circular DNA plasmids expanded in disabled E.coli strains and then purified
 
**Plasmids express the foreign gene insert at the site of injection
 
**Can be vaccinated directly into the host
 
 
===Adjuvants===
 
 
*Used with vaccines containing inactivated organisms which alone only stimulate a weak immune response
 
  
*Some create a depot of [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]] at the injection site allowing a steady flow of [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]] into the afferent lymph
+
LA vaccines produce a superior response to disease than using killed organisms as the dose of antigen is larger and more sustained, and the response takes place at the site of natural infection, producing a greater local response than with killed organism vaccines. Examples include:
 +
*The current vaccine for tuberculosis (called BCG) contains an attenuated form of a mycobacteria
 +
*Vaccines for leishmaniasis
 +
*Vaccines for parainfluenza virus 3 of calves is developed to be temperature-sensitive so that it grows at 34 C in the upper respiratory tract but not at 38 C in the lungs
  
*Some stimulate the immune system to amplify the adaptive immune response to [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigens]]
+
'''Killed inactivated organism or toxin (toxoid)''' are useful where virulent and toxic organisms cannot be used as vaccines as they would cause disease. Organisms can be killed using radiation or chemicals so that they still possess the antigens to stimulate an immune response, but the organisms are unable to replicate inside the host. Alternatively, toxins are inactivated to produce a toxoid which will still have the antigens needed to produce an immune response but will not be harmful to the host. Two doses are required to [[B_cell_differentiation#Primary_T_Cell_Dependent_Response|'''prime''']] the immune system initially, and then induce an immunoligical [[B_cell_differentiation#Secondary_T_Cell_Dependent_Response|'''memory''']] of the disease causing organism.
**E.g. Pathogen-associated molecular patterns (PAMPs)
 
**E.g. PAMP-like adjuvants which assist naive [[Lymphocytes - WikiBlood#T cells|T cell]] priming
 
  
*Different subtypes of [[Lymphocytes - WikiBlood#Helper CD4+|T helper cells]] are stimulated by different adjuvants
+
1:4000 formaldehyde is used in the preparation of killed vaccines; inactivants containing azuridines and beta propiolactone are being developed which do not leave a persistent infectious viral fraction (like formaldehyde).
**E.g. Aluminium salts generate bias [[T cell differentiation - WikiBlood#TH2 Cells|T helper II]] responses for [[Immunoglobulins - WikiBlood|'''antibody''']]-mediated immunity
 
**E.g. Killed mycobacteria generate IL-12 producing good '''cell'''-mediated immunity
 
  
*Adjuvants decrease the number of injections needed and the amount of [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigen]] administered
+
===Subunits (part of the organism)===
 +
These can be purified proteins such as a single envelope protein separated from a purified virus by detergent and then centrifuged (traditional method) - genetic engineering can now make single protein vaccines quickly and accurately.
  
===Marker Vaccines===
+
Recombinant or synthetic proteins can also be used as a subunit - the gene for the antigen required is inserted into a virus vector or cloned into bacteria allowing endogenous expression of the antigen. Small antigens, such as peptides, can be produced synthetically where necessary e.g. with Influenza viruses that are constantly mutating, and Canary pox vaccines encoding rabies or FeLV spike proteins (canary pox is safe as it undergoes incomplete replication in mammalian skin cells).
  
*Distinguish infected from vaccinated animals
+
Subunit antigens can also be isolated using the DNA coding for antigenic proteins; circular DNA plasmids are expanded in disabled E.coli strains and then purified - the plasmids expressing the foreign gene can be vaccinated directly into the host.
  
*Have a deleted protein or gene
+
==Adjuvants==
 +
Adjuvants are used with vaccines containing inactivated organisms which alone would only stimulate a weak immune response. Some adjuvants create a depot of antigen at the injection site allowing a steady flow of antigen into the afferent lymph, while others stimulate the immune system to amplify the adaptive immune response to antigens e.g. pathogen-associated molecular patterns (PAMPs). PAMP-like adjuvants assist naive [[T cells|T cell]] priming.
  
*Vaccinated animals cannot make antibody to the missing protein whereas infected animals can
+
Different subtypes of [[T cell differentiation|T helper cells]] are stimulated by different adjuvants, for example:
 +
*Aluminium salts generate bias [[T cell differentiation#TH2 Cells|T helper II]] responses for antibody-mediated immunity
 +
*Killed mycobacteria generate IL-12 producing good '''cell'''-mediated immunity
  
*Helps immunosurveillance for animals infected by a virus in countries that vaccinate against the virus
+
Adjuvants decrease the number of injections needed and the amount of antigen that needs to be administered, but they have been associated with vaccine reactions.
  
==Which type of vaccine is used for each disease?==
+
==Marker Vaccines==
 +
Marker vaccines distinguish infected from vaccinated animals in disease control programmes.
 +
They contain a deleted protein or gene; vaccinated animals cannot make antibody to the missing protein whereas infected animals can and this helps immunosurveillance for animals infected by an organism in countries that vaccinate against that disease.
  
*The life-cycle of the organisms needs to be understood to ascertain the best type of immune response for fighting the particular infection
+
==Tailoring Vaccines for Specific Diseases==
 +
*The life-cycle of infectious organisms needs to be understood to ascertain the best type of immune response for fighting that particular infection
 +
*A vaccine can be created to provide the specific immunity best suited for fighting the associated infection
  
*A vaccine can be created to provide specific immunity which is best suited for fighting the specific infection
+
===Immunity to Viral Infection===
 +
[[Image:Virus Life Cycle.jpg|thumb|right|200px|Virus Life Cycle - Copyright Dr Brian Catchpole BVetMed PhD MRCVS]]
 +
The virus life cycle consists of an extracellular phase, a replicative intracellular phase and another extracellular phase spreading viral particles to other cells to begin the life cycle again
  
===Immunity to Virus Infection===
+
Immunity for the extracellular phase requires neutralising [[Immunoglobulins - Overview|'''antibody''']]:
[[Image:Virus Life Cycle.jpg|thumb|right|150px|Virus Life Cycle - Copyright Dr Brian Catchpole BVetMed PhD MRCVS]]
+
*[[B cells]] needed
*The virus life cycle consists of an extracellular phase, a replicative intracellular phase and another extracellular phase spreading viral particles to other cells to begin the life cycle again
+
*[[T cell differentiation#TH2 Cells|T helper type II cells]] needed (for the [[Major Histocompatability Complexes#MHC II|MHC class II pathway]])
 +
*Live, killed and subunit vaccines can be used
  
*Immunity for the extracellular phase requires neutralising [[Immunoglobulins - WikiBlood|'''antibody''']]
+
Immunity for the intracellular phase requires [[T_cells#Cytotoxic_CD8.2B|CD8+ cytotoxic T lymphocytes (CTL)]] and uses the [[Major Histocompatability Complexes#MHC I|MHC class I pathway]].
**[[Lymphocytes - WikiBlood#B Cells|B cells]] needed
+
*Only live vaccine can be used to get into cells (entering via the endogenous pathway)
**[[T cell differentiation - WikiBlood#TH2 Cells|T helper type II cells]] needed (for the [[MHC - WikiBlood#MHC II|MHC class II pathway]])
 
**Live vaccine can be used
 
**Killed vaccine can be used
 
**Subunit vaccine can be used
 
 
 
*Immunity for the intracellular phase requires [[Lymphocytes - WikiBlood#Cytotoxic CD8+|'''CD8+ cytotoxic T cells''']]
 
**[[MHC - WikiBlood#MHC I|MHC class I pathway]]
 
**Only live vaccine can be used to get into cells (entering via the endogenous pathway)
 
  
 
===Immunity to Bacterial Infection===
 
===Immunity to Bacterial Infection===
  
*Extracellular bacterial infection need [[Immunoglobulins - WikiBlood|'''antibody''']] production for [[Complement - WikiBlood#Opsonisation|opsonisation]] and to activate the [[Complement - WikiBlood|complement pathways]]
+
*Extracellular bacterial infection needs antibody production for [[Complement#Opsonisation|opsonisation]] and to activate the [[Complement|complement pathways]]
**[[Lymphocytes - WikiBlood#B Cells|B cells]] needed
+
*[[B cells]] are needed
**[[T cell differentiation - WikiBlood#TH2 Cells|T helper type II cells]] needed
+
*[[T cell differentiation#TH2 Cells|T helper type II cells]] are needed
  
*Vesicular infections can only be cured by organisms being destroyed inside [[Macrophages - WikiBlood|'''macrophages''']]
+
Vesicular infections can only be cured by organisms being destroyed inside [[Macrophages|'''macrophages''']]
**[[T cell differentiation - WikiBlood#TH1 Cells|T helper type I cells]] needed
+
*[[T cell differentiation#TH1 Cells|T helper type I cells]] are needed
  
 
==When do we vaccinate?==
 
==When do we vaccinate?==
[[Image:Colostrum Intake.jpg|right|thumb|150px|Colostrum Intake - Copyright Prof Dirk Werling DrMedVet PhD MRCVS]]
+
[[Image:Colostrum Intake.jpg|right|thumb|200px|Colostrum Intake - Copyright Prof Dirk Werling DrMedVet PhD MRCVS]]
[[Image:Vaccinating puppies with Parvo.jpg|right|thumb|150px|Response to vaccination against canine parvovirus depending on antibody titre of puppies - Copyright Prof Dirk Werling DrMedVet PhD MRCVS]]
+
[[Image:Vaccinating puppies with Parvo.jpg|right|thumb|200px|Response to vaccination against canine parvovirus depending on antibody titre of puppies - Copyright Prof Dirk Werling DrMedVet PhD MRCVS]]
*Usually when animals are young
+
*Breeding females can be vaccinated so that immunity is passively transferred to their offspring via the [[Materno-Fetal Immunity - Introduction#Passive transfer via colostrum|colostrum]] - this protects neonates for the first 8-12 weeks of life.
 
 
*Breeding females so immunity is passed to offspring via the [[Materno-fetal immunity - WikiBlood#Passive transfer via colostrum|colostrum]]
 
**Protects neonates for the first 8-12 weeks of life
 
  
*Vaccination of young animals should be when the natural passive immunity decreases below the threshold for providing protection. Active immunity should then be stimulated so that the animal has constant protection. The vaccination should not be given too early, as the natural immunity can interfere with immunisation by binding and neutralising the vaccine [[Adaptive Immune System - WikiBlood#Antigen Recognition|antigens]].
+
*Vaccination of young animals should be when the natural passive immunity decreases below the threshold for providing protection. Active immunity should then be stimulated so that the animal has sustained protection. If vaccination is given too early, the natural immunity can interfere with immunisation by binding and neutralising the vaccine antigens.
  
*2 vaccines are usually given to allow for differences between neonates as the point where natural immunity decreases and active immunity needs to be stimulated, will differ between littermates and between different animals
+
*Two vaccines are usually given to allow for differences between individual animals in the time taken for any natural immunity to decrease.
  
 
===Dog Vaccinations===
 
===Dog Vaccinations===
 +
Diseases routinely covered by vaccination include:
  
'''Diseases covered by Vaccination'''
+
*[[Canine Parvovirus]]
 
 
*Canine [[Parvoviridae|Parvovirus]]
 
  
*Canine Distemper
+
*[[Canine Distemper Virus|Canine Distemper]]
  
*Canine Infectious Hepatitis
+
*[[Infectious Canine Hepatitis]]
  
*Leptospirosis
+
*[[Leptospirosis - Cats and Dogs|Leptospirosis]]
  
*Canine Parainfluenza virus
+
*[[Canine Parainfluenza - 2|Canine Parainfluenza virus]]
  
*Kennel Cough  
+
*[[Canine Infectious Tracheobronchitis|Kennel Cough]]
  
*Rabies  
+
*[[Rabies]]
  
 
'''When to Vaccinate'''
 
'''When to Vaccinate'''
  
*Puppies are usually first vaccinated between 6 to 8 weeks of age
+
Puppies are usually first vaccinated between 6 to 8 weeks of age; a second vaccination is given 3-4 weeks later. Younger puppies (less than 16 weeks old) may require the third booster 3-4 weeks later, making the vaccination schedule to end between 14 to 16 weeks old. Adult dogs need booster vaccination regularly (depending on the specific vaccination and the recommendations of the vaccine manufacturer).
**A second vaccination is needed 2 weeks later
 
 
 
*Adult dogs need booster vaccination regularly (depending on the specific vaccination)
 
  
 
===Cat Vaccinations===
 
===Cat Vaccinations===
[[Image:Sebby cat.jpg|thumb|right|150px|Cat - Copyright nabrown RVC]]
+
[[Image:Sebby cat.jpg|thumb|right|175px|Cat - Copyright nabrown RVC]]
 
'''Diseases covered by Vaccination'''
 
'''Diseases covered by Vaccination'''
  
*Feline Infectious Enteritis
+
*Feline Infectious Enteritis ([[Feline Panleucopenia]])
  
*Feline Infectious Respiratory Disease 'Cat Flu'
+
*'Cat Flu', including Feline [[Feline Herpesvirus 1|Herpesvirus]] and [[Feline Calicivirus]]
**Feline [[Herpesviridae|Herpesvirus]]
 
**Feline [[Caliciviridae|Calicivirus]]
 
  
*[[Immunodeficiencies - WikiBlood#Feline Leukaemia Virus (FeLV)|Feline Leukaemia virus]]
+
*[[Feline Leukaemia Virus]] (FeLV)
**Killed whole virus (only used in USA)
 
**Purified subunit
 
**Recombinant subunit
 
**Recombinant canarypox
 
  
*[[Immunodeficiencies - WikiBlood#Feline Immunodeficiency Virus (FIV)|Feline Infectious Viraemia]]  
+
*[[Feline Immunodeficiency Virus]]  
**Killed whole virus containing A and D subtypes (only used in USA)
 
  
*Feline Chlamydophilosis
+
* [[Chlamydiosis, Feline|Feline chlamydiosis]] (Chlamydophila felis)
  
 
'''When to Vaccinate'''
 
'''When to Vaccinate'''
 
+
Kittens are usually vaccinated around 9 weeks old and a second vaccination is given 3 weeks later. Adult cats need booster vaccination regularly (depending on the specific vaccination and the vaccine manufacturers recommendations).
*Kittens are usually vaccinated around 9 weeks old
 
**A second vaccination is needed 3 weeks later
 
 
 
*Adult cats need booster vaccination regularly (depending on the specific vaccination)
 
  
 
===Rabbit Vaccinations===
 
===Rabbit Vaccinations===
[[Image:Buzz bunny.jpg|thumb|right|150px|Rabbit - Copywright L. Drew RVC]]
+
[[Image:Buzz bunny.jpg|thumb|right|200px|Rabbit - Copywright L. Drew RVC]]
 
'''Diseases covered by Vaccination'''
 
'''Diseases covered by Vaccination'''
  
*Viral Haemorrhagic Disease
+
*[[Vaccinations_for_Rabbits#VHD_Vaccination|Viral Haemorrhagic Disease]]
  
*[[Poxviruses#Leporipoxviruses|Myxomatosis]]
+
*[[Vaccinations_for_Rabbits#Myxomatosis_Vaccination|Myxomatosis]]
  
 
'''When to Vaccinate'''
 
'''When to Vaccinate'''
 +
Rabbits can be vaccinated against [[Myxomatosis|myxomatosis]] from 6 weeks of age and VHD from 2½ to 3 months of age. Booster vaccinations are given every 12 months. In areas at high risk of myxomatosis, it is recommended to give myxomatosis boosters at six-monthly intervals. Some myxomatosis vaccines need to given [[Vaccinations_for_Rabbits#Myxomatosis_Vaccination|partially intradermally]].
  
*Rabbits can be vaccinated against [[Poxviruses#Leporipoxviruses|Myxomatosis]] from 6 weeks of age
+
==Vaccine Failure==
 +
Failures do occur and should be reported on the VMD [http://www.vmd.gov.uk/ 'yellow form' MLA252A] if the events occur in the United Kingdom. Vaccine failures in other European Union (EU) Member States, Norway, Iceland and Liechtenstein should be reported to the relevant competent authority where the event occurred using the [http://www.ema.europa.eu/ema/index.jsp?curl=pages/regulation/document_listing/document_listing_000176.jsp&mid=WC0b01ac058002ddcb/ EU reporting forms for veterinarians] which are available in each EU language on the [http://www.ema.europa.eu/ European Medicines Agency] website. Circumstances leading to vaccine failures include:
 +
*Recipient is already infected with the virus or is immunosuppressed and unable to mount an immune response.
  
*HVD from 2½ to 3 months of age 
+
*Break down of the '''cold-chain''' during transport (incorrect storage of vaccines requiring refrigeration)
  
*Booster vaccinations are given every 12 months. In areas at high risk of myxomatosis, it is recommended to give myxomatosis boosters at six-monthly intervals.
+
*Improper administration (e.g.myxomatosis vaccine)
  
==Vaccine Failure==
+
*Accidental mixing of inactivated and live vaccines in the same syringe
 +
 
 +
*Recipient has maternal antibody to the vaccine
  
*Recipient is already infected with the virus or immunosuppressed
+
*Immunity waning due to missed booster vaccination
  
*Break down of the '''cold-chain''' during transport
+
*Vaccine is damaged during manufacture
  
*Improper administration
+
{{Learning
 +
|flashcards = [[Vaccination Flashcards|Vaccination Flashcards]]
 +
|full text =[http://www.cabi.org/cabdirect/FullTextPDF/2009/20093258316.pdf '''Factors influencing vaccine efficacy - a general review.''' Rashid, A.; Rasheed, K.; Akhtar, M.; Pakistan Agricultural Scientists Forum, Lahore, Pakistan, JAPS, Journal of Animal and Plant Sciences, 2009, 19, 1, pp 22-25, 18 ref.]
  
*Mixing of inactivated and live vaccines in the same syringe
+
[http://www.cabi.org/cabdirect/FullTextPDF/2009/20093115229.pdf ''' Establishing vaccine protocols - focus on client communication.''' Datz, C.; The North American Veterinary Conference, Gainesville, USA, Small animal and exotics. Proceedings of the North American Veterinary Conference, Orlando, Florida, USA, 17-21 January, 2009, 2009, pp 608-611]
  
*Recipient has maternal antibody to the vaccine
+
[http://www.cabi.org/cabdirect/FullTextPDF/2009/20093115232.pdf '''Feline lifestyle vaccination protocols.''' Lappin, M. R.; The North American Veterinary Conference, Gainesville, USA, Small animal and exotics. Proceedings of the North American Veterinary Conference, Orlando, Florida, USA, 17-21 January, 2009, 2009, pp 621-624, 18 ref.]
  
*Not enough animals vaccinated
+
[http://www.cabi.org/cabdirect/FullTextPDF/2007/20073166574.pdf '''Immunological basis of vaccination.''' Lunn, D. P.; The North American Veterinary Conference, Gainesville, USA, Large animal. Proceedings of the North American Veterinary Conference, Volume 21, Orlando, Florida, USA, 2007, 2007, pp 135-137]
  
*Boosters not done
+
[http://www.cabi.org/cabdirect/FullTextPDF/2007/20073166575.pdf '''Equine vaccines: what works, what doesn't?''' Lunn, D. P.; The North American Veterinary Conference, Gainesville, USA, Large animal. Proceedings of the North American Veterinary Conference, Volume 21, Orlando, Florida, USA, 2007, 2007, pp 138-140]
  
*Vaccine is counterfeit or homeopathic
+
|Vetstream = [https://www.vetstream.com/canis/Content/Freeform/fre00859.asp Vaccination Protocol]
 +
}}
  
 
==Links==
 
==Links==
 
+
:[[:Category:Viral Organisms|Viruses A to Z]]
*[[Clinical Case 3|Myxomatosis Clinical Case]]
+
:[[:Category:Bacterial Organisms|Bacteria A to Z]]
 
 
*[[Viruses|Viruses A to Z]]
 
  
 
==References==
 
==References==
  
 
'''Textbooks'''
 
'''Textbooks'''
 
 
*Ivan Roitt: '''Essential Immunology,''' Ninth edition
 
*Ivan Roitt: '''Essential Immunology,''' Ninth edition
  
 
'''Lecture Notes'''
 
'''Lecture Notes'''
 
 
*Dr Brian Catchpole BVetMed PhD MRCVS
 
*Dr Brian Catchpole BVetMed PhD MRCVS
 
 
*Dr Peter H Russell BVSc MSc PhD MRCVS FRCPath
 
*Dr Peter H Russell BVSc MSc PhD MRCVS FRCPath
  
==Creators==
+
<br><br>
 
+
{{Jim Bee 2007}}
[[Natalie Brown]]
+
[[Category:Immunology]]
 
 
<big><center>[[Immunology - WikiBlood|'''BACK TO IMMUNOLOGY''']]</center></big>
 
<big><center>[[Host invasion by microorganisms - WikiBlood|'''BACK TO HOST INVASION BY MICROORGANISMS''']]</center></big>
 

Latest revision as of 17:02, 4 June 2016

Syringe

Source: Wikimedia Commons; Author: ZaldyImg (2008)

Introduction

Why do we vaccinate animals?

  • To protect against infectious diseases
  • Where there is no effective treatment once infected e.g. FeLV, FIV
  • Where disease is life-threatening e.g. Canine Parvovirus
  • To prevent the spread of disease by virus excretion e.g. Rabies, FMDV

The goal is to vaccinate 90% of the population to reduce the amount of endemic virus until no new infections occur. Once the disease risk is low, vaccination can be replaced by an eradication or quarantine programme.

How do vaccines work?

Vaccination induces an immunological memory of the infectious organism. High levels of cytotoxic T cells and neutralising antibody are activated 24 - 48 hours post vaccination as a secondary response (instead of 4-10 days later as a primary response). Neutralising antibody then blocks the attachment of the infectious organism to host cell receptors.

Endogenous vaccines cause antigens to be made as new proteins by the cell, bacterium or virus and involves MHC class I processing live virus, recombinant virus or DNA vaccines.

Exogenous vaccines are when the antigen is processed from the outside by endocytosis without any new proteins being made by the host cell. This involves MHC class II processing inactivated and subunit vaccines.

Route of Administration

  • Usually by subcutaneous injection for systemic protection. Some vaccines such as the myxomatosis vaccine NobivacTM Myxo (Intervet UK Ltd) require an intradermal injection as part of the administration procedure.
  • For a localised mucosal immune response, intranasal administration is required (IgA) e.g. kennel cough vaccine.

Vaccination Options

Passive Immunisation - Copyright nabrown RVC

Passive immunisation

Advantages

  • Immediate protection

Disadvantages:

  • Short duration of action; temporary protection is obtained by the administration of preformed antibody from another individual of the same or of a different species. The acquired antibodies are used in combination with antigen, and catabolised by the body, meaning protection is gradually lost over time
  • Injection of antiserum may cause an allergic response
  • Antiserum contains many antibodies, not just the specific antibodies needed

Types of antibodies administered:

  • Maternally-derived antibodies in colostrum when there is a failure of passive transfer of Immunoglobulin G
  • Antiserum
    • The antibodies are used in combination with antigen (and often an adjuvant) which is injected into a host animal
    • The immune system of that animal synthesises antibodies
    • Repeated injections at intervals increases total antibody production
    • The immunised animal is bled and the serum collected which contains the newly made antibodies. The serum is called antiserum.
    • The serum can then be injected into a different animal to confer passive immunisation


Examples of passive immunisation:

INFECTION HUMAN SOURCE OF ANTIBODY EQUINE SOURCE OF ANTIBODY USE
Tetanus Diptheria Used Used Prophylaxis treatment
Botulism Not used Used Treatment
Venomous bite Not used Used Treatment
Rabies Used Not used Post-exposure to virus



Active Immunisation - Copyright nabrown RVC

Active immunisation

Active immunisation requires the administration of antigen so the patient develops their own antibodies to protect against disease. Suitable antigens include:

  • Living organisms
  • Dead organisms
  • Toxoids
  • Subunit antigens
  • DNA

Advantages

  • Long duration of action; once antibody is produced against the antigen, memory cells are formed which continue circulating in the body

Disadvantages

  • The host's immune system needs to evoke an immune response against the antigen which can take a few days
  • Can require two or more doses to be effective; the first dose initiates the priming reaction where antibody production ceases after a few weeks, but the second and subsequent doses create memory cells which remain in the circulation for a much longer period of time.

Vaccine Antigens

Potential antigenic substances include:

Whole Organism

Live Attenuated (LA) vaccines include the organism but in an altered form - virulent organisms cannot be used as vaccines as they have the potential to cause disease. Virulence is reduced by growing the organism in altered conditions (e.g. in cells or eggs), so that it is less able to replicate when introduced into the host, and is therefore less likely to cause disease. Virulence can also be reduced by genetic engineering, or by using naturally occurring avirulent strains.


LA vaccines produce a superior response to disease than using killed organisms as the dose of antigen is larger and more sustained, and the response takes place at the site of natural infection, producing a greater local response than with killed organism vaccines. Examples include:

  • The current vaccine for tuberculosis (called BCG) contains an attenuated form of a mycobacteria
  • Vaccines for leishmaniasis
  • Vaccines for parainfluenza virus 3 of calves is developed to be temperature-sensitive so that it grows at 34 C in the upper respiratory tract but not at 38 C in the lungs

Killed inactivated organism or toxin (toxoid) are useful where virulent and toxic organisms cannot be used as vaccines as they would cause disease. Organisms can be killed using radiation or chemicals so that they still possess the antigens to stimulate an immune response, but the organisms are unable to replicate inside the host. Alternatively, toxins are inactivated to produce a toxoid which will still have the antigens needed to produce an immune response but will not be harmful to the host. Two doses are required to prime the immune system initially, and then induce an immunoligical memory of the disease causing organism.

1:4000 formaldehyde is used in the preparation of killed vaccines; inactivants containing azuridines and beta propiolactone are being developed which do not leave a persistent infectious viral fraction (like formaldehyde).

Subunits (part of the organism)

These can be purified proteins such as a single envelope protein separated from a purified virus by detergent and then centrifuged (traditional method) - genetic engineering can now make single protein vaccines quickly and accurately.

Recombinant or synthetic proteins can also be used as a subunit - the gene for the antigen required is inserted into a virus vector or cloned into bacteria allowing endogenous expression of the antigen. Small antigens, such as peptides, can be produced synthetically where necessary e.g. with Influenza viruses that are constantly mutating, and Canary pox vaccines encoding rabies or FeLV spike proteins (canary pox is safe as it undergoes incomplete replication in mammalian skin cells).

Subunit antigens can also be isolated using the DNA coding for antigenic proteins; circular DNA plasmids are expanded in disabled E.coli strains and then purified - the plasmids expressing the foreign gene can be vaccinated directly into the host.

Adjuvants

Adjuvants are used with vaccines containing inactivated organisms which alone would only stimulate a weak immune response. Some adjuvants create a depot of antigen at the injection site allowing a steady flow of antigen into the afferent lymph, while others stimulate the immune system to amplify the adaptive immune response to antigens e.g. pathogen-associated molecular patterns (PAMPs). PAMP-like adjuvants assist naive T cell priming.

Different subtypes of T helper cells are stimulated by different adjuvants, for example:

  • Aluminium salts generate bias T helper II responses for antibody-mediated immunity
  • Killed mycobacteria generate IL-12 producing good cell-mediated immunity

Adjuvants decrease the number of injections needed and the amount of antigen that needs to be administered, but they have been associated with vaccine reactions.

Marker Vaccines

Marker vaccines distinguish infected from vaccinated animals in disease control programmes. They contain a deleted protein or gene; vaccinated animals cannot make antibody to the missing protein whereas infected animals can and this helps immunosurveillance for animals infected by an organism in countries that vaccinate against that disease.

Tailoring Vaccines for Specific Diseases

  • The life-cycle of infectious organisms needs to be understood to ascertain the best type of immune response for fighting that particular infection
  • A vaccine can be created to provide the specific immunity best suited for fighting the associated infection

Immunity to Viral Infection

Virus Life Cycle - Copyright Dr Brian Catchpole BVetMed PhD MRCVS

The virus life cycle consists of an extracellular phase, a replicative intracellular phase and another extracellular phase spreading viral particles to other cells to begin the life cycle again

Immunity for the extracellular phase requires neutralising antibody:

Immunity for the intracellular phase requires CD8+ cytotoxic T lymphocytes (CTL) and uses the MHC class I pathway.

  • Only live vaccine can be used to get into cells (entering via the endogenous pathway)

Immunity to Bacterial Infection

Vesicular infections can only be cured by organisms being destroyed inside macrophages

When do we vaccinate?

Colostrum Intake - Copyright Prof Dirk Werling DrMedVet PhD MRCVS
Response to vaccination against canine parvovirus depending on antibody titre of puppies - Copyright Prof Dirk Werling DrMedVet PhD MRCVS
  • Breeding females can be vaccinated so that immunity is passively transferred to their offspring via the colostrum - this protects neonates for the first 8-12 weeks of life.
  • Vaccination of young animals should be when the natural passive immunity decreases below the threshold for providing protection. Active immunity should then be stimulated so that the animal has sustained protection. If vaccination is given too early, the natural immunity can interfere with immunisation by binding and neutralising the vaccine antigens.
  • Two vaccines are usually given to allow for differences between individual animals in the time taken for any natural immunity to decrease.

Dog Vaccinations

Diseases routinely covered by vaccination include:

When to Vaccinate

Puppies are usually first vaccinated between 6 to 8 weeks of age; a second vaccination is given 3-4 weeks later. Younger puppies (less than 16 weeks old) may require the third booster 3-4 weeks later, making the vaccination schedule to end between 14 to 16 weeks old. Adult dogs need booster vaccination regularly (depending on the specific vaccination and the recommendations of the vaccine manufacturer).

Cat Vaccinations

Cat - Copyright nabrown RVC

Diseases covered by Vaccination

When to Vaccinate Kittens are usually vaccinated around 9 weeks old and a second vaccination is given 3 weeks later. Adult cats need booster vaccination regularly (depending on the specific vaccination and the vaccine manufacturers recommendations).

Rabbit Vaccinations

Rabbit - Copywright L. Drew RVC

Diseases covered by Vaccination

When to Vaccinate Rabbits can be vaccinated against myxomatosis from 6 weeks of age and VHD from 2½ to 3 months of age. Booster vaccinations are given every 12 months. In areas at high risk of myxomatosis, it is recommended to give myxomatosis boosters at six-monthly intervals. Some myxomatosis vaccines need to given partially intradermally.

Vaccine Failure

Failures do occur and should be reported on the VMD 'yellow form' MLA252A if the events occur in the United Kingdom. Vaccine failures in other European Union (EU) Member States, Norway, Iceland and Liechtenstein should be reported to the relevant competent authority where the event occurred using the EU reporting forms for veterinarians which are available in each EU language on the European Medicines Agency website. Circumstances leading to vaccine failures include:

  • Recipient is already infected with the virus or is immunosuppressed and unable to mount an immune response.
  • Break down of the cold-chain during transport (incorrect storage of vaccines requiring refrigeration)
  • Improper administration (e.g.myxomatosis vaccine)
  • Accidental mixing of inactivated and live vaccines in the same syringe
  • Recipient has maternal antibody to the vaccine
  • Immunity waning due to missed booster vaccination
  • Vaccine is damaged during manufacture


Vaccines Learning Resources
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Full Text Articles
Full text articles available from CAB Abstract
(CABI log in required)
Factors influencing vaccine efficacy - a general review. Rashid, A.; Rasheed, K.; Akhtar, M.; Pakistan Agricultural Scientists Forum, Lahore, Pakistan, JAPS, Journal of Animal and Plant Sciences, 2009, 19, 1, pp 22-25, 18 ref.

Establishing vaccine protocols - focus on client communication. Datz, C.; The North American Veterinary Conference, Gainesville, USA, Small animal and exotics. Proceedings of the North American Veterinary Conference, Orlando, Florida, USA, 17-21 January, 2009, 2009, pp 608-611

Feline lifestyle vaccination protocols. Lappin, M. R.; The North American Veterinary Conference, Gainesville, USA, Small animal and exotics. Proceedings of the North American Veterinary Conference, Orlando, Florida, USA, 17-21 January, 2009, 2009, pp 621-624, 18 ref.

Immunological basis of vaccination. Lunn, D. P.; The North American Veterinary Conference, Gainesville, USA, Large animal. Proceedings of the North American Veterinary Conference, Volume 21, Orlando, Florida, USA, 2007, 2007, pp 135-137

Equine vaccines: what works, what doesn't? Lunn, D. P.; The North American Veterinary Conference, Gainesville, USA, Large animal. Proceedings of the North American Veterinary Conference, Volume 21, Orlando, Florida, USA, 2007, 2007, pp 138-140


Links

Viruses A to Z
Bacteria A to Z

References

Textbooks

  • Ivan Roitt: Essential Immunology, Ninth edition

Lecture Notes

  • Dr Brian Catchpole BVetMed PhD MRCVS
  • Dr Peter H Russell BVSc MSc PhD MRCVS FRCPath



LIVE logo Originally funded by the RVC Jim Bee Award 2007